pXJ42-p110 CUX1
(Plasmid
#100814)
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PurposePlasmid vector expressing human p110 CUX1 (amino acids 878-1505 of P39880), with an N-terminal Myc tag and a C-terminal HA tag.
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Depositing Lab
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Depositing OrganizationMcGill University
Located in Canada -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 100814 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 100814-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 100814-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepXJ42
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Modifications to backbonepXJ42 was obtained from Alan Wildeman. pXJ42 was derived from pXJ40 (Cell 65:551-568, (1991) by changing the polylinker. It contains the human CMV promoter and intron 2 from the rabbit beta-globin gene We used pXJ42 originally because we needed a mammalian vector that did not contain the origin of replication of SV40. In practice, people in the lab found this was the best vector and we continued to use it for other applications.
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namep110 CUX1 (amino acids 878-1505)
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SpeciesH. sapiens (human)
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MutationContains amino acids 878-1505
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GenBank IDP39880
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Entrez GeneCUX1 (a.k.a. CASP, CDP, CDP/Cut, CDP1, COY1, CUTL1, CUX, Clox, Cux/CDP, GDDI, GOLIM6, Nbla10317, p100, p110, p200, p75)
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Tags
/ Fusion Proteins
- Myc (N terminal on insert)
- HA (C terminal on insert)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 100814-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 100814-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pXJ42-p110 CUX1 was a gift from Alain Nepveu (Addgene plasmid # 100814 ; http://n2t.net/addgene:100814 ; RRID:Addgene_100814) -
For your References section:
p110 CUX1 cooperates with E2F transcription factors in the transcriptional activation of cell cycle-regulated genes. Truscott M, Harada R, Vadnais C, Robert F, Nepveu A. Mol Cell Biol. 2008 May;28(10):3127-38. doi: 10.1128/MCB.02089-07. Epub 2008 Mar 17. 10.1128/MCB.02089-07 PubMed 18347061
Map uploaded by the depositor.
