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talen_logo.png TALEN Kits Available from Addgene

TALENs are important new tools for genome engineering. Fusions of transcription activator-like (TAL) effectors of plant pathogenic Xanthomonas spp. to the FokI nuclease, TALENs bind and cleave DNA in pairs. Binding specificity is determined by customizable arrays of polymorphic amino acid repeats in the TAL effectors. Click here to view Addgene's TALEN Guide.

These collections and accompanying documentation allow one to efficiently assemble TALEN constructs with custom repeat arrays.


TALEN Kit Description Depositing PI
FusX TALEN Assembly System Contains 336 pFusX clones allowing for Golden Gate assembly of 15-16 RVD TALEN inserts in one cloning step rather than two. Stephen Ekker
Golden Gate
Golden Gate cloning method.
Validated in multiple organisms.
Dan Voytas
Adam Bogdanove
Joung Lab TAL Effector Engineering Reagents Assembly via serial ligation.
Validated in zebrafish somatic cells.
Keith Joung
Zhang Lab
TALE Toolbox
PCR/Golden Gate cloning method.
Optimized for human expression.
Feng Zhang
LIC TAL Effector Assembly Kit Assembly by ligation-independent cloning (LIC). Validated in human cells. Veit Hornung
Kit comprised of 834 plasmids of pre-assembled trimer and tetramer RVD domains designed for rapid TALEN assembly. Kiran Musunuru
and Chad Cowan
Ekker Lab TALEN Kit All possible tetramers (264) of RVDs, compatible with Golden Gate TALEN and TAL Effector Kit 2.0. Stephen Ekker
Platinum Gate TALEN Kit Platinum TALENs assembled by Golden Gate. Validated in several species. Takashi Yamamoto

Additional Plasmids for Use with Addgene TALEN Kits

For Use With: Plasmid(s) Depositing PI
Golden Gate
RCIscript-GoldyTALEN and pC-GoldyTALEN Daniel Carlson
Golden Gate
TAL5-BB and pTAL6-BB Tom Ellis
Golden Gate
pCS2TAL3-DD and pCS2TALE3-RR David Grunwald
Golden Gate
pCAG-T7-TALEN(Sangamo)-Destination Constructs Pawel Pelczar
Golden Gate
TALEN Construction Accessory Pack Takashi Yamamoto
Golden Gate
TALE-transcription activation destination vectors Charles Gersbach
Golden Gate
Destination vectors for TALE-mediated Genome Visualization (TGV) Maria-Elena Torres-Padilla
Golden Gate
pTAL7a and pTAL7b, for the application of TALEN technology in hPSCs Boris Greber
Joung Lab REAL Assembly TALEN Kit TALE-LSD1 plasmids for epigenome editing
(EMM65, EMM67, & EMM71T)
Bradley Bernstein
and Eric Mendenhall
Golden Gate
pBlue-TAL for the generation of germline mutations in Bombyx mori and Drosophila melanogaster Michal Zurovec
Zhang Lab TALE Toolbox TALEColor plasmids: destination vectors for use in labeling specific repetitive DNA sequences in human chromosomes. Thoru Pederson

See all available Golden Gate Add-on plasmids.

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Do you have suggestions for other plasmids that should be added to this list?

Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource!