TALEN Kits Available from Addgene
TALENs are important new tools for genome engineering. Fusions of transcription activator-like (TAL) effectors of plant pathogenic Xanthomonas spp. to the FokI nuclease, TALENs bind and cleave DNA in pairs. Binding specificity is determined by customizable arrays of polymorphic amino acid repeats in the TAL effectors. Click here to view Addgene's TALEN Guide.
These collections and accompanying documentation allow one to efficiently assemble TALEN constructs with custom repeat arrays.
TALEN Kits
| TALEN Kit | Description | Depositing PI |
|---|---|---|
| FusX TALEN Assembly System | Contains 336 pFusX clones allowing for Golden Gate assembly of 15-16 RVD TALEN inserts in one cloning step rather than two. | Stephen Ekker |
| Golden Gate TALEN 2.0 |
Golden Gate cloning method. Validated in multiple organisms. |
Dan Voytas
Adam Bogdanove |
| Joung Lab TAL Effector Engineering Reagents | Assembly via serial ligation. Validated in zebrafish somatic cells. |
Keith Joung |
| Zhang Lab TALE Toolbox |
PCR/Golden Gate cloning method. Optimized for human expression. |
Feng Zhang |
| LIC TAL Effector Assembly Kit | Assembly by ligation-independent cloning (LIC). Validated in human cells. | Veit Hornung |
| Musunuru/Cowan Lab TALEN Kit |
Kit comprised of 834 plasmids of pre-assembled trimer and tetramer RVD domains designed for rapid TALEN assembly. | Kiran Musunuru and Chad Cowan |
| Ekker Lab TALEN Kit | All possible tetramers (264) of RVDs, compatible with Golden Gate TALEN and TAL Effector Kit 2.0. | Stephen Ekker |
| Platinum Gate TALEN Kit | Platinum TALENs assembled by Golden Gate. Validated in several species. | Takashi Yamamoto |
Additional Plasmids for Use with Addgene TALEN Kits
See all available Golden Gate Add-on plasmids.
Do you have suggestions for other plasmids that should be added to this list?
Fill out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!