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TALEN Kits Available from Addgene

Addgene is a global, non-profit plasmid repository dedicated to making it easier for scientists to share.

TALENs are important new tools for genome engineering. Fusions of transcription activator-like (TAL) effectors of plant pathogenic Xanthomonas spp. to the FokI nuclease, TALENs bind and cleave DNA in pairs. Binding specificity is determined by customizable arrays of polymorphic amino acid repeats in the TAL effectors. Click here to view Addgene's TALEN Guide.

These collections and accompanying documentation allow one to efficiently assemble TALEN constructs with custom repeat arrays.

TALEN Kits:

TALEN Kit
Description
Depositing PI
Golden Gate
TALEN 2.0
Golden Gate cloning method.
Validated in multiple organisms.

Dan Voytas

Adam Bogdanove

Joung Lab TAL Effector Engineering Reagents
Assembly via serial ligation.
Validated in zebrafish somatic cells.
Keith Joung
Zhang Lab
TALE Toolbox
PCR/Golden Gate cloning method.
Optimized for human expression.
Feng Zhang
LIC TAL Effector Assembly Kit
Assembly by ligation-independent cloning (LIC). Validated in human cells.
Veit Hornung
Musunuru/Cowan
Lab TALEN Kit
Kit comprised of 834 plasmids of pre-assembled trimer and tetramer RVD domains designed for rapid TALEN assembly.
Kiran Musunuru
and Chad Cowan
Ekker Lab TALEN Kit
All possible tetramers (264) of RVDs, compatible with Golden Gate TALEN and TAL Effector Kit 2.0.
Stephen Ekker
Platinum Gate TALEN Kit
Platinum TALENs assembled by Golden Gate. Validated in several species.
Takashi Yamamoto

Additional Plasmids for Use with Addgene TALEN Kits:

For Use With:
Plasmid(s)
Depositing PI
Golden Gate
TALEN 2.0
RCIscript-GoldyTALEN and pC-GoldyTALEN
Daniel Carlson
Golden Gate
TALEN 2.0
TAL5-BB and pTAL6-BB
Tom Ellis
Golden Gate
TALEN 2.0
pCS2TAL3-DD and pCS2TALE3-RR
David Grunwald
Golden Gate
TALEN 2.0
pCAG-T7-TALEN(Sangamo)-Destination Constructs
Pawel Pelczar
Golden Gate
TALEN 2.0
TALEN Construction and Evaluation Accessory Pack
Takashi Yamamoto
Golden Gate
TALEN 2.0
TALE-transcription activation destination vectors
Charles Gersbach
Golden Gate
TALEN 2.0
Destination vectors for TALE-mediated Genome Visualization (TGV)
Maria-Elena Torres-Padilla
Golden Gate
TALEN 2.0
pTAL7a and pTAL7b, for the application of TALEN technology in hPSCs
Boris Greber
Joung Lab REAL Assembly TALEN Kit
TALE-LSD1 plasmids for epigenome editing
(EMM65, EMM67, & EMM71T)
Bradley Bernstein
and Eric Mendenhall
Golden Gate
TALEN 2.0
pBlue-TAL for the generation of germline mutations in Bombyx mori and Drosophila melanogaster
Michal Zurovec
Zhang Lab TALE Toolbox
TALEColor plasmids: destination vectors for use in labeling specific repetitive DNA sequences in human chromosomes.
Thoru_Pederson

See all available Golden Gate Add-on plasmids.


Do you have suggestions for other plasmids or categories that should be added to this list?
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Request Plasmids

To order, Login or Register a new account. Add the kit or individual plasmids to your cart, then complete the checkout process online. Click here for additional ordering information.

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