Addgene: TALEN Kits

TALEN Kits Available from Addgene

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TALENs are important new tools for genome engineering. Fusions of transcription activator-like (TAL) effectors of plant pathogenic Xanthomonas spp. to the FokI nuclease, TALENs bind and cleave DNA in pairs. Binding specificity is determined by customizable arrays of polymorphic amino acid repeats in the TAL effectors. Click here to view Addgene's TALEN Guide.

These collections and accompanying documentation allow one to efficiently assemble TALEN constructs with custom repeat arrays.

TALEN Kits:

TALEN Kit	Description	Depositing PI
Golden Gate TALEN 2.0	Golden Gate cloning method. Validated in multiple organisms.	Dan Voytas Adam Bogdanove
Joung Lab TAL Effector Engineering Reagents	Assembly via serial ligation. Validated in zebrafish somatic cells.	Keith Joung
Zhang Lab TALE Toolbox	PCR/Golden Gate cloning method. Optimized for human expression.	Feng Zhang
LIC TAL Effector Assembly Kit	Assembly by ligation-independent cloning (LIC). Validated in human cells.	Veit Hornung
Musunuru/Cowan Lab TALEN Kit	Kit comprised of 834 plasmids of pre-assembled trimer and tetramer RVD domains designed for rapid TALEN assembly.	Kiran Musunuru and Chad Cowan

Additional Plasmids for Use with Addgene TALEN Kits:

For Use With:	Plasmid(s)	Depositing PI
Golden Gate TALEN 2.0	RCIscript-GoldyTALEN and pC-GoldyTALEN	Daniel Carlson
Golden Gate TALEN 2.0	TAL5-BB and pTAL6-BB	Tom Ellis
Golden Gate TALEN 2.0	pCS2TAL3-DD and pCS2TALE3-RR	David Grunwald
Golden Gate TALEN 2.0	pCAG-T7-TALEN(Sangamo)-Destination Constructs	Pawel Pelczar
Golden Gate TALEN 2.0	TALEN Construction and Evaluation Accessory Pack	Takashi Yamamoto
Golden Gate TALEN 2.0	TALE-transcription activation destination vectors	Charles Gersbach
Golden Gate TALEN 2.0	Destination vectors for TALE-mediated Genome Visualization (TGV)	Maria-Elena Torres-Padilla
Golden Gate TALEN 2.0	pTAL7a and pTAL7b, for the application of TALEN technology in hPSCs	Boris Greber
Joung Lab REAL Assembly TALEN Kit	TALE-LSD1 plasmids for epigenome editing (EMM65, EMM67, & EMM71T)	Bradley Bernstein and Eric Mendenhall
Golden Gate TALEN 2.0	pBlue-TAL for the generation of germline mutations in Bombyx mori and Drosophila melanogaster	Michal Zurovec
Zhang Lab TALE Toolbox	TALEColor plasmids: destination vectors for use in labeling specific repetitive DNA sequences in human chromosomes.	Thoru_Pederson

See all available Golden Gate Add-on plasmids.

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