Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at help@addgene.org. Learn more

talen_logo.png TALEN Kits Available from Addgene


TALENs are important new tools for genome engineering. Fusions of transcription activator-like (TAL) effectors of plant pathogenic Xanthomonas spp. to the FokI nuclease, TALENs bind and cleave DNA in pairs. Binding specificity is determined by customizable arrays of polymorphic amino acid repeats in the TAL effectors. Click here to view Addgene's TALEN Guide.

These collections and accompanying documentation allow one to efficiently assemble TALEN constructs with custom repeat arrays.

TALEN Kits

TALEN Kit Description Depositing PI
Golden Gate
TALEN 2.0
Golden Gate cloning method.
Validated in multiple organisms.
Dan Voytas
Adam Bogdanove
Joung Lab TAL Effector Engineering Reagents Assembly via serial ligation.
Validated in zebrafish somatic cells.
Keith Joung
Zhang Lab
TALE Toolbox
PCR/Golden Gate cloning method.
Optimized for human expression.
Feng Zhang
LIC TAL Effector Assembly Kit Assembly by ligation-independent cloning (LIC). Validated in human cells. Veit Hornung
Musunuru/Cowan
Lab TALEN Kit
Kit comprised of 834 plasmids of pre-assembled trimer and tetramer RVD domains designed for rapid TALEN assembly. Kiran Musunuru
and Chad Cowan
Ekker Lab TALEN Kit All possible tetramers (264) of RVDs, compatible with Golden Gate TALEN and TAL Effector Kit 2.0. Stephen Ekker
Platinum Gate TALEN Kit Platinum TALENs assembled by Golden Gate. Validated in several species. Takashi Yamamoto

Additional Plasmids for Use with Addgene TALEN Kits

For Use With: Plasmid(s) Depositing PI
Golden Gate
TALEN 2.0
RCIscript-GoldyTALEN and pC-GoldyTALEN Daniel Carlson
Golden Gate
TALEN 2.0
TAL5-BB and pTAL6-BB Tom Ellis
Golden Gate
TALEN 2.0
pCS2TAL3-DD and pCS2TALE3-RR David Grunwald
Golden Gate
TALEN 2.0
pCAG-T7-TALEN(Sangamo)-Destination Constructs Pawel Pelczar
Golden Gate
TALEN 2.0
TALEN Construction Accessory Pack Takashi Yamamoto
Golden Gate
TALEN 2.0
TALE-transcription activation destination vectors Charles Gersbach
Golden Gate
TALEN 2.0
Destination vectors for TALE-mediated Genome Visualization (TGV) Maria-Elena Torres-Padilla
Golden Gate
TALEN 2.0
pTAL7a and pTAL7b, for the application of TALEN technology in hPSCs Boris Greber
Joung Lab REAL Assembly TALEN Kit TALE-LSD1 plasmids for epigenome editing
(EMM65, EMM67, & EMM71T)
Bradley Bernstein
and Eric Mendenhall
Golden Gate
TALEN 2.0
pBlue-TAL for the generation of germline mutations in Bombyx mori and Drosophila melanogaster Michal Zurovec
Zhang Lab TALE Toolbox TALEColor plasmids: destination vectors for use in labeling specific repetitive DNA sequences in human chromosomes. Thoru_Pederson

See all available Golden Gate Add-on plasmids.

Do you have suggestions for other plasmids or categories that should be added to this list?

Email help@addgene.org to help us improve this resource!