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COVID-19 and Coronavirus Plasmids & Resources


Please Note: If you are interested in ordering or depositing plasmids related to COVID-19 research, please place your order or submit your deposit online and also email us at [email protected] to let us know and we will prioritize your order or deposit. Thank you!


The global research community is moving quicky to expand the knowledge and understanding of COVID-19 and related coronaviruses. To assist with this effort Addgene will maintain this plasmid collection page, which outlines various plasmids available and those coming soon. Additionally, we have linked to collections of open-access articles, protocols, and other resource collections related to COVID-19 that may be of use to scientists.

Background

The Coronavirus Disease-2019 (COVID-19) pandemic is caused by a novel virus strain from the Coronaviridae family called SARS-CoV-2. Little is known about the biology of this particular virus but studies on other viruses of the family such as SARS-CoV and MERS-CoV showed that cell entry of coronaviruses depends on binding of the viral spike (S) proteins to cellular receptors. Another key step is the priming of the S protein by host cell proteases. SARS-CoV, for instance, enters into the cell via hACE2, an angiotensin receptor, and its S protein is primed by the serine protease TMPRSS2.

Plasmids

SARS-CoV-2 Plasmids - Now available and more coming soon.

  • Plasmids containing SARS-CoV-2 sequences are currently undergoing quality control analysis in our lab and will be made available as soon as possible. Some of these plasmids are described in the following preprints:
    • The sequence of human ACE2 is suboptimal for binding the S spike protein of SARS coronavirus 2. Procko E. bioRxiv 2020.03.16.994236
    • A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug Targets and Potential Drug-Repurposing. Gordon, DE et al. bioRxiv 2020.03.22.002386

  • Addgene has partnered with Gingko Bioworks to distribute SARS-CoV-2 plasmids. To read more about this partnership and the plasmids that will be available, please see the Gingko Bioworks Blog post: Synthesizing DNA to fight coronavirus. Estimated availibility date for these plasmids is June 2020.

The following plasmids containing SARS-CoV-2 sequences are now available:

ID Plasmid Description PI
141183 pcDNA3-SARS-CoV-2-S-RBD-Fc Mammalian expression plasmid for RBD of SARS-CoV-2 protein S fused with IgG1-Fc Procko
141184 pcDNA3-SARS-CoV-2-S-RBD-sfGFP Mammalian expression plasmid for RBD of SARS-CoV-2 protein S fused with sfGFP Procko
141255 pDONR207 SARS-CoV-2 NSP1 Gateway-compatible Entry vector, with insert of NSP1 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1 Roth
141257 pDONR207 SARS-CoV-2 NSP3 Gateway-compatible Entry vector, with insert of NSP3 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1 Roth
141258 pDONR223 SARS-CoV-2 NSP4 Gateway-compatible Entry vector, with insert of NSP4 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1 Roth
141259 pDONR223 SARS-CoV-2 NSP5 Gateway-compatible Entry vector, with insert of NSP5 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1 Roth
141260 pDONR223 SARS-CoV-2 NSP6 Gateway-compatible Entry vector, with insert of NSP6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1 Roth
141261 pDONR223 SARS-CoV-2 NSP7 Gateway-compatible Entry vector, with insert of NSP7 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1 Roth
141262 pDONR223 SARS-CoV-2 NSP8 Gateway-compatible Entry vector, with insert of NSP8 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1 Roth
141263 pDONR223 SARS-CoV-2 NSP9 Gateway-compatible Entry vector, with insert of NSP9 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1 Roth
141266 pDONR223 SARS-CoV-2 NSP13 Gateway-compatible Entry vector, with insert of NSP13 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1 Roth
141267 pDONR223 SARS-CoV-2 NSP14 Gateway-compatible Entry vector, with insert of NSP14 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1 Roth
141268 pDONR223 SARS-CoV-2 NSP15 Gateway-compatible Entry vector, with insert of NSP15 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1 Roth
141269 pDONR223 SARS-CoV-2 NSP16 Gateway-compatible Entry vector, with insert of NSP16 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1 Roth
141271 pDONR207 SARS-CoV-2 ORF3A Gateway-compatible Entry vector, with insert of ORF3A gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1 Roth
141272 pDONR223 SARS-CoV-2 ORF3B Gateway-compatible Entry vector, with insert of ORF3B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1 Roth
141273 pDONR207 SARS-CoV-2 E Gateway-compatible Entry vector, with insert of E gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1 Roth
141274 pDONR207 SARS-CoV-2 M Gateway-compatible Entry vector, with insert of M gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1 Roth
141275 pDONR223 SARS-CoV-2 ORF6 Gateway-compatible Entry vector, with insert of ORF6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1 Roth
141276 pDONR207 SARS-CoV-2 ORF7A Gateway-compatible Entry vector, with insert of ORF7A gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1 Roth
141277 pDONR223 SARS-CoV-2 ORF7B Gateway-compatible Entry vector, with insert of ORF7B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1 Roth
141278 pDONR223 SARS-CoV-2 ORF8 Gateway-compatible Entry vector, with insert of ORF8 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1 Roth
141280 pDONR223 SARS-CoV-2 ORF9B Gateway-compatible Entry vector, with insert of ORF9B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1 Roth

SARS-CoV Plasmids (also called "SARS-classic")

Due to export control regulations, SARS-CoV materials are available only in the US at this time.

Plasmids Encoding Human Genes or Inserts

Several human genes have been identified as having a key role in coronovirus infection, such as ACE2 and TMPRSS2 (entry of SARS-CoV and SARS-CoV-2) and DPP4 (entry of MERS-CoV). Find plasmids related to these genes below.

ID Plasmid Description PI
1786 hACE2 Mammalian expression of human ACE2 Choe
11249 hCathepsin B Mammalian expression of human cathepsin B Choe
11250 hCathepsin L Mammalian expression of human cathepsin L Choe
11251 hCathepsin S Mammalian expression of human cathepsin S Choe
31713 pRK5F-TACE Mammalian expression of human TACE (ADAM17) Derynck
141185 pCEP4-myc-ACE2 Mammalian expression plasmid for human ACE2 with an N-terminal (extracellular) c-myc tag Procko
53887 TMPRSS2 Expression of TMPRSS2 Reeves
31110 ACE2(-1119)-luc Luciferase reporter construct for human ACE2 promoter Ryffel
31111 ACE2(-252)-luc Luciferase reporter construct for human ACE2 promoter Ryffel
31112 ACE2(-202)-luc Luciferase reporter construct for human ACE2 promoter Ryffel
91601 pPN066 Express sgRNA targeting human DPP4 (receptor of MERS-CoV) Barrett
91602 pPN067 Express sgRNA targeting human DPP4 (receptor of MERS-CoV) Barrett
82122 pDONR223_FURIN_WT Gateway Donor vector containing human FURIN Boehm, Hahn, Root
91613 pPN088 Express sgRNA targeting human furin Barrett
91614 pPN089 Express sgRNA targeting human furin Barrett
53896 PCP4_pCSdest Expression of PCP4, involved in beating of airway cilia Reeves

General Plasmid Tools

Find plasmid tools that may be helpful for COVID-19 research in the table below:

ID Plasmid Description PI
90097 pC013 - Twinstrep-SUMO-huLwCas13a huLwCas13a for recombinant protein bacterial expression Zhang
91909 pC019 - LwCas13a from Leptotrichia wadei Expresses LwCas13a for bacterial expression and contains backbone for spacer cloning Zhang
113431 pMBP-LbCas12a Expresses 10xHis-MBP fused to LbCas12a Doudna
109029 pET-6xHis-SUMO-MarathonRT Expresses a highly processive reverse transcriptase called MarathonRT Pyle
78234 pMLRT Expresses Murine Leukemia Reverse Transcriptase (MLRT) Voigt
102786 pET_RTX_(exo-) Expresses the Reverse Transcriptase gene (3'-5' exonuclease deficient mutant) Ellington
102787 pET_RTX Expresses the Reverse Transcriptase gene Ellington
124429 PCDNA3-FlipGFP(TEV cleavage seq) T2A mCherry Caspase reporter construct Shu

Do you have a suggestion for plasmids that would be useful for your COVID-19 research? Fill out this Suggest a Plasmid form. If the plasmid has been described previously we will reach out to the lab to ask them to deposit the plasmid with us. Otherwise we will pass along the request to Ginkgo Bioworks for consideration for synthesis.

External Resources

Research Articles

Many publishers have allowed free access to articles related to SARS-CoV-2 research during the current the outbreak:

Collections of COVID-19 related articles spanning multiple publishers can be found here:

Protocols

Protocols relevant to SARS-CoV-2 Research

More Coronovirus Resources

Do you have suggestions for other plasmids that should be added to this list?

Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource!