Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

SARS-CoV-2 Spike Receptor Binding Domain Deep Mutational Scanning Library


(Pooled Library #1000000172)

  • Purpose

    This pair of duplicate libraries contains SARS-CoV-2 spike receptor binding domain (RBD) mutants in a yeast surface display vector.

    Each library is barcoded with randomized N16 barcodes downstream of the coding sequence.

    These libraries can be used for comprehensive mapping of the biochemical effects of RBD mutations.

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 1000000172 SARS-CoV-2_RBD_DMS_Library 1 $500 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Mutagenized CDS
    SARS-CoV-2 Receptor Binding Domain
  • Mutagenized region size
    603 bp
  • Total insert size
    1040 bp
  • Effective diversity
    ~150,000

Library Shipping

This library will be delivered as two pooled DNA libraries in microcentrifuge tubes on blue ice (tubes labeled lib1 and lib2). The tube contents will not necessarily be frozen. For best results, minimize freeze-thaws.

For each library tube, you will receive:

  • Volume
    ∼20µL
  • Concentration
    25ng/µL

Depositor Comments

SARS-CoV-2 RBD DMS Library
  • Bloom Lab SARS-CoV-2 RBD DMS Library

  • DOI:10.1016/j.cell.2020.08.012
    Figure S1. Available for the public to copy, distribute, or display under
    the Creative Commons Attribution 4.0 International license. (CC BY 4.0)

The depositing laboratory provides the following GitHub repositories

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SARS-CoV-2 Spike Receptor Binding Domain Deep Mutational Scanning Library was a gift from Jesse Bloom (Addgene #1000000172)
  • For your References section:

    Deep Mutational Scanning of SARS-CoV-2 Receptor Binding Domain Reveals Constraints on Folding and ACE2 Binding. Starr TN, Greaney AJ, Hilton SK, Ellis D, Crawford KHD, Dingens AS, Navarro MJ, Bowen JE, Tortorici MA, Walls AC, King NP, Veesler D, Bloom JD Cell. 2020 Sep 3;182(5):1295-1310.e20. doi: 10.1016/j.cell.2020.08.012. Epub 2020 Aug 11. PubMed 32841599