Chemogenetics Plasmids
Background
Chemogenetics is a technique that uses genetically engineered receptors to selectively control and investigate cell signaling pathways. Chemogenetic receptors are activated by small molecules, enabling remote control of signaling within cells expressing these engineered receptors. Chemogenetic receptors are popular in neuroscience, where they can be used to interrogate brain function and examine the relationship between neuronal signaling and behavior. These receptors also have broad applications in several fields and have been used to investigate a variety of cell types and mechanisms.
Schematic depicting the typical workflow of chemogenetics plasmid use in research
Chemogenetics Plasmids Available from Addgene
Addgene has a large collection of chemogenetics plasmids that can be used to interrogate cell function in various contexts. We also now offer ready-to-use AAV preparations of select chemogenetic plasmids, which can be used directly for in vivo or in vitro studies. Browse our list of chemogenetics plasmids below. Plasmids with the viral service icon are also offered as viral preparations.
Click on different properties to create a custom filtered list of the chemogenetics plasmids in Addgene's collection.
ID | Name | Promoter | Receptor | Plasmid Type | Activity | PI |
---|---|---|---|---|---|---|
50469 | pAAV-CaMKIIa-EGFP ![]() |
CaMKIIa | none | AAV, Non-cre | EGFP expression control | Roth |
50476 | pAAV-CaMKIIa-hM3D(Gq)-mCherry ![]() |
CaMKIIa | hM3D(Gq) | AAV, Non-cre | Neuronal burst firing | Roth |
50477 | pAAV-CaMKIIa-hM4D(Gi)-mCherry ![]() |
CaMKIIa | hM4D(Gi) | AAV, Non-cre | Neuronal silencing | Roth |
65418 | pAAV-CaMKIIa-HA-KORD-IRES-mCitrine | CaMKIIa | KORD | AAV, Non-cre | Neuronal silencing | Roth |
50459 | pAAV-hSyn-DIO-mCherry ![]() |
Syn1 | none | AAV, Cre | mCherry expression control | Roth |
44361 | pAAV-hSyn-DIO-hM3D(Gq)-mCherry ![]() |
Syn1 | hM3D(Gq) | AAV, Cre | Neuronal burst firing | Roth |
44362 | pAAV-hSyn-DIO-hM4D(Gi)-mCherry ![]() |
Syn1 | hM4D(Gi) | AAV, Cre | Neuronal silencing | Roth |
50458 | pAAV-hSyn-DIO-rM3D(Gs)-mCherry | Syn1 | hM3D(Gs) | AAV, Cre | cAMP production | Roth |
50465 | pAAV-hSyn-EGFP ![]() |
Syn1 | none | AAV, Non-cre | EGFP expression control | Roth |
50457 | pAAV-hSyn-DIO-EGFP ![]() |
Syn1 | none | AAV, Cre | EGFP expression control | Roth |
50474 | pAAV-hSyn-hM3D(Gq)-mCherry ![]() |
Syn1 | hM3D(Gq) | AAV, Non-cre | Neuronal burst firing | Roth |
50475 | pAAV-hSyn-hM4D(Gi)-mCherry ![]() |
Syn1 | hM4D(Gi) | AAV, Non-cre | Neuronal silencing | Roth |
65417 | pAAV-hSyn-dF-HA-KORD-IRES-mCitrine ![]() |
Syn1 | KORD | AAV, Cre | Neuronal silencing | Roth |
50473 | pAAV-GFAP-EGFP | GFAP | none | AAV, Non-cre | EGFP expression control | Roth |
58909 | pAAV-GFAP104-mCherry | GFAP | none | AAV, Non-cre | mCherry expression control | Boyden |
50478 | pAAV-GFAP-hM3D(Gq)-mCherry ![]() |
GFAP | hM3D(Gq) | AAV, Non-cre | Neuronal burst firing | Roth |
50479 | pAAV-GFAP-hM4D(Gi)-mCherry ![]() |
GFAP | hM4D(Gi) | AAV, Non-cre | Neuronal silencing | Roth |
75033 | pAAV CD68-hM4D(Gi)-mCherry | CD68 | hM4D(Gi) | AAV, Non-cre | Neuronal silencing | Roth |
50454 | pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine | Syn1 | hM3D(Gq) | AAV, Cre | Neuronal burst firing, cell filling | Roth |
50455 | pAAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine | Syn1 | hM4D(Gi) | AAV, Cre | Neuronal silencing, cell filling | Roth |
50456 | pAAV-hSyn-DIO-HA-hM3D(Gs)-IRES-mCitrine | Syn1 | hM3D(Gs) | AAV, Cre | cAMP production, cell filling | Roth |
50460 | pAAV-EF1a-DIO-hM3D(Gq)-mCherry | EF1a | hM3D(Gq) | AAV, Cre | Neuronal burst firing | Roth |
50461 | pAAV-EF1a-DIO-hM4D(Gi)-mCherry | EF1a | hM4D(Gi) | AAV, Cre | Neuronal silencing | Roth |
50462 | pAAV-EF1a-DIO-mCherry | EF1a | none | AAV, Cre | mCherry expression control | Roth |
50463 | pAAV-hSyn-HA-hM3D(Gq)-IRES-mCitrine | Syn1 | hM3D(Gq) | AAV, Non-cre | Neuronal burst firing, cell filling | Roth |
50464 | pAAV-hSyn-HA-hM4D(Gi)-IRES-mCitrine | Syn1 | hM4D(Gi) | AAV, Non-cre | Neuronal silencing, cell filling | Roth |
50466 | pAAV-CaMKIIa-HA-hM3D(Gq)-IRES-mCitrine | CaMKIIa | hM3D(Gq) | AAV, Non-cre | Neuronal burst firing, cell filling | Roth |
50467 | pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine | CaMKIIa | hM4D(Gi) | AAV, Non-cre | Neuronal silencing, cell filling | Roth |
50468 | pAAV-CaMKIIa-HA-rM3D(Gs)-IRES-mCitrine | CaMKIIa | rM3D(Gs) | AAV, Non-cre | cAMP production, cell filling | Roth |
50470 | pAAV-GFAP-HA-hM3D(Gq)-IRES-mCitrine | GFAP | hM3D(Gq) | AAV, Non-cre | Neuronal burst firing, cell filling | Roth |
50471 | pAAV-GFAP-HA-hM4D(Gi)-IRES-mCitrine | GFAP | hM4D(Gi) | AAV, Non-cre | Neuronal silencing, cell filling | Roth |
50472 | pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine | GFAP | rM3D(Gs) | AAV, Non-cre | cAMP production, cell filling | Roth |
45547 | pcDNA5/FRT-HA-hM3D(Gq) | CMV | hM3D(Gq) | Mammalian expression | Inositol monophosphate (IP1) accumulation, ERK1/2 phosophorylation | Roth |
45548 | pcDNA5/FRT-HA-hM4D(Gi) | CMV | hM4D(Gi) | Mammalian expression | Neuronal silencing | Roth |
45549 | pcDNA5/FRT-HA-rM3D(Gs) | CMV | rM3D(Gs) | Mammalian expression | Roth | |
70717 | AAV-mOXT-hM3Dq-mCherry-WPRE | oxytocin | hM3D(Gq) | AAV, Non-cre | Geschwind | |
66795 | pAAV-PTRE-tight-hM3Dq-mCherry | tet-inducible | hM3D(Gq) | AAV, Non-cre | Wisden | |
81008 | pAAV-CBA-DIO-hM4Di-mCherry | CBA | hM4D(Gi) | AAV, Cre | Sabatini | |
24429 | pcDNA3-Rog | CMV | RASSL | Mammalian expression | Conklin | |
24503 | pcDNA3-Ro2 | CMV | RASSL | Mammalian expression | Conklin | |
24504 | pcDNA3-Ro1 | CMV | RASSL | Mammalian expression | Conklin | |
16312 | pUNIV-SIG-5HTR4D100A | h5HT4b | Mammalian expression | Conklin | ||
16313 | pUHG10.3-TetO-Rs1 | TetO | h5HT4b | Mammalian expression | Conklin |
Currently, no available plasmids.
Browse all Chemogenetics Plasmids
G Protein Chimera Plasmids
G protein chimeras can be used to alter the signalling phenotype of G-protein coupled receptors (GPCRs). These chimeras have been used to study receptor signalling, ligand binding, effector coupling, and other molecular mechanisms of GPCRs. For a user manual on these plasmids, see the supplemental document or user manual created by the depositor.
ID | Name | Gene | Mutation | Depositor Comments | PI |
---|---|---|---|---|---|
24502 | q4wt | Gq alpha | none | This is Gq alpha with an HA epitope engineered into an internal site that does not seem to affect receptor coupling in multiple studies | Conklin |
24498 | qs5 | Gq alpha | Replacement of the five carboxyl-terminal amino acids of Gq alpha with those of Gs alpha | This construct allows some Gs-coupled receptors to stimulate phospholipase C | Conklin |
24501 | qi5 | Gq alpha | Gq alpha with the C-terminal amino acids changed from Gq alpha to Gi alpha residues | This construct allows many Gi-coupled receptors to stimulate phospholipase C | Conklin |
24500 | qo5 | Gq alpha | Gq alpha with the C-terminal amino acids changed from Gq alpha to Go alpha residues | Works the same as qi5 but (for unknown reasons) has a slightly lower basal PLC activity | Conklin |
25867 | qz5 | Gq alpha | Replacement of the five carboxyl-terminal amino acids of Gq alpha with those of Gz alpha | Works the same as qi5 | Conklin |
24499 | sq5 | Gs alpha | This is Gs alpha with the C-terminal amino acids changed from Gs alpha to Gq alpha residues | This construct allows some Gq-coupled receptors to stimulate Adenylate cyclase | Conklin |

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