Addgene - CAG::PSAMQ79G,Q139G:5HT3HC-IRES-GFP Plasmid Data

Plasmid 32474: CAG::PSAMQ79G,Q139G:5HT3HC-IRES-GFP

Gene/insert name:
PSAMQ79G,Q139G:5HT3 HC
Insert size:
1415
Species:
H. sapiens (human), M. musculus (mouse)
Fusion protein or tag:
IRES-EGFP
Terminal:
C terminal on backbone
Vector backbone:
pCAGGS- IRES-GFP
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
7200
Modifications to Backbone:
WPRE (Woodchuck hepatitis virus posttranscriptional regulatory element) before polyA sequence
Promoter:
CAG
Cloning site 5':
EcoRV
Site destroyed during cloning:
Yes
Cloning site 3':
EcoRV
Site destroyed during cloning:
Yes
5' sequencing primer:
CTGCTAACCATGTTCATGC List of Sequencing Primers
3' sequencing primer:
CAGAAACGCAAGAGTCTTC
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Scott Sternson
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Chemical and genetic engineering of selective ion channel-ligand interactions. Magnus et al (Science. 2011 Sep 2;333(6047):1292-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32474" in your Materials and Methods section.