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Addgene Zebrafish Collection Icon Zebrafish Plasmids and Resources


The zebrafish (Danio rerio), named for the horizontal stripes on the sides of the adult body, is a tropical freshwater fish in the minnow family. It has long been established as a powerful vertebrate model organism for the study of genetics and developmental biology. With its rapid development (embryogenesis is complete in ~3 days), short generation time (~3 months), ease of care and breeding, and transparency of the embryo and larvae, it is easy to culture large populations that are amenable to genetics, high-throughput screening and non-invasive live imaging. A standard toolbox of genetic methods, such as Tol2 transposons, Cre-mediated site-specific recombination, morpholinos, zinc finger nucleases, TALENS, and CRISPR/Cas9-based gene editing make the zebrafish a stalwart research model. With more than 70% of human disease genes having an ortholog in zebrafish, this species has more recently emerged as a model system for human disease and translational research, including the study of cancer, inflammation and infection, regeneration, human neurological disorders, microbiota studies, and drug discovery and development. Here we highlight some of the plasmids and tools available at Addgene for use in zebrafish.


Browse Plasmids

Plasmid Tools for Use in Zebrafish

Looking for plasmids containing your zebrafish gene of interest? Search Addgene's collection for plasmids that contain a zebrafish gene or sequence.

The table below highlights plasmids that contain tools for use in zebrafish research. This includes plasmids for genome editing, empty backbones, fluorescent proteins, reporters, biosensors, and Cre expression plasmids. Large collections or kits are described below.

ID Plasmid Purpose Gene/Insert PI
17095pCS2P+ Kirschner
26125I-SceI pB 5UAS::ZfSyGCaMP2Synaptophysin GCaMP2 (Danio rerio) Lagnado
27320pENTR5'_ubizebrafish ubiquitin promoter (Danio rerio) Zon
27321pENTR/D_creERt2creERt2 Zon
27322pENTR5'_ubi:loxP-EGFP-loxPubiquitin:loxP-EGFP-loxP (Danio rerio) Zon
27323pDestTol2pA2_ubi:EGFPubiquitin:EGFP reporter (Danio rerio) Zon
29574tol2-mpx-Dendra2mpx-Dendra 2 Huttenlocher
29585tol2-mpx-mCherrympx-mCherry Huttenlocher
31241pSCAC-69Mitochondrial localization of EGFP, for real-time imaging of mitochondria in transgenic zebrafishmitochondrial localization enhanced green fluorescent protein (Danio rerio) Choi
32359pBH-UAS-secA5-YFPLive imaging of apoptotic cells in zebrafishsecreted human annexin 5 (Homo sapiens) Peterson
32673pISce1-memGFP Tsang
32674pISce1-D2EGFP Tsang
37845E1b-GFP-Tol2 Ahituv
37846E1b-GFP-Tol2-Gateway Ahituv
41878tol2-4xnr UAS-PA Rac1-mCherryphotoactivatable Rac1 (Homo sapiens) Huttenlocher
42250DR274guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcription Joung
42252MLM3639Streptococcus pyogenes Cas9-3X Flag Joung
45246Tol2-mpx:Lifeact-Rubylifeact-ruby Huttenlocher
45247mpx:UtrCH-GFPutrophin (Homo sapiens) Huttenlocher
45509Tol2 Krt4 Src (YF)-uniRapR-ceruleanSrc (YF)-uniRapR-cerulean Huttenlocher
46138pFT1.0mCherry Chen
46139pFT1.1mCherry Chen
46140pFT1.2mCherry Chen
46144pT2-eab2-[GTR]EGFP/mCherry Chen
46759pT7-gRNAgRNA cloning vector for in vitro transcription of target gRNAgRNA core (Synthetic) Chen
47322pCS2-Cas9For in vivo expression of Cas9 or generation of Cas9 mRNACas9 (Other) Schier
47929pCS2-nCas9nexpression of an optimized Cas9 for genome-editing in zebrafishnls-zcas9-nls (Synthetic) Chen
51815pCS2+hSpCas9Encode hSpCas9 for in vitro transcription with Sp6humanized S.pyogenes Cas9 Kinoshita
53616Zebrafish ArcLight Q175Genetically encoded voltage sensor ArcLight with improved kinetics Zebrafish ArcLight-Q175 (Danio rerio) Pieribone
58890p5'Entry-lck-5.5kbprovides zebrafish lck promoter (5.5 kb) for GateWay construction of Tol2 transgeneszebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 5.5 kb (Danio rerio) Yoder
58891p5'Entry-lck-7.4kbprovides zebrafish lck promoter (7.4 kb) for GateWay construction of Tol2 transgeneszebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 7.4 kb (Danio rerio) Yoder
59530Tol2-elavl3-H2B-GCaMP6sExpressed nuclear-localized GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backboneelavl3 promoter (Danio rerio), H2B (Homo sapiens), GCaMP6s (Synthetic) Ahrens
59531Tol2-elavl3-GCaMP6sExpressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backboneelavl3 promoter (Danio rerio), GCaMP6s (Synthetic) Ahrens
60877pMAZeReporter construct for lineage tracing and mosaic analysis in zebrafish.hsp70l promoter, Cre, dTomato, UAS promoter, Gal4VP16 Lewis
61069eGFPbait-E2A-KalTA4-pA donor vectorfor CRISPR/Cas9 mediated insertion of E2A-KalTA4; to be used in combination with a eGFP specific sgRNA (e.g. Plasmid 61051)eGFPbait (Synthetic), KalTA4 (Synthetic) Del Bene
61387pCS2-NfsB.zf1mRNA synthesis of bacterial nitroreductase NfsB codon modified for zebrafishnfsB.zf1 (Synthetic) Burgess
61388pCS2-Tol1.zf1mRNA synthesis of tol1 transposase codon modified for zebrafishtol1.zf1 (Synthetic) Burgess
61389pCS2-Cer.zf1mRNA synthesis of cerulean codon modified for zebrafishCer.zf1 (Synthetic) Burgess
61390pCS2-TagRFPT.zf1mRNA synthesis of TagRFPT codon modified for zebrafishTagRFPT.zf1 (Synthetic) Burgess
61391pCS2-Cre.zf1mRNA synthesis of Cre recombinase codon modified for zebrafishCre.zf1 (Synthetic) Burgess
61392pCS2-Gal4ff.zf1mRNA synthesis of Gal4ff codon modified for zebrafishGal4ff.zf1 (Synthetic) Burgess
61394pBS-Isce1-eno2:egfpExpresses GFP in neurons of transgenic zebrafisheno2 regulatory element (Danio rerio) Burton
62213pCS2-epNTRepNTR (zebrafish optimized nitroreductase, T41L, N71S, F124W) in pCS2epNTR (Synthetic) Burgess
62215pT1UciMPUAS transgenesis vector Burgess
62216pT1QciMPQUAS transgenesis vector Burgess
62542pCS2-nCas9n-nanos 3’UTRexpression of a germ-line targeted, codon optimized Cas9 for genome-editing in zebrafishnanos 3' UTR (Danio rerio) Giraldez
63154pME-Cas9Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLSCas9 (Danio rerio) Zon
63155pME-Cas9-T2A-GFPMiddle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.Cas9-T2A-GFP (Danio rerio) Zon
63156pDestTol2CG2-U6:gRNADestination vector for Gateway based on vector #395 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter, and cmlc2:GFP as a transgenesis marker. Zon
63157pDestTol2pA2-U6:gRNADestination vector for Gateway based on vector #394 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter. Zon
64020p5E_unc5035' Gateway entry vector with muscle-specific promoter of zebrafish unc-45bmuscle-specific promoter of zebrafish unc-45b (Danio rerio) Currie
64021punc503GFP503-bp promoter of zebrafish unc-45b drives muscle-specific GFP expressionunc-45b: GFP (Danio rerio) Currie
64022pDestTol2pACryGFPGateway destination vector with zebrafish aA-crystallin promoter driving GFP in lensGFP (Danio rerio) Currie
64023pDESTtol2pACrymCherryGateway destination vector with zebrafish aA-crystallin promoter driving mCherry GFP in lensmCherry (Danio rerio) Currie
64237pME-Cas9expresses codon optimized Cas9 in zebrafishcodon optimized Cas9 (Other) Chen
65562th-P2A-EGFP donorIntron targeting-mediated EGFP knockin at the zebrafish th locus.th-P2A-EGFP (Danio rerio) Du
65563th-P2A-Gal4 donorIntron targeting-mediated Gal4 knockin at the zebrafish th locus. th-P2A-Gal4 (Danio rerio) Du
65564gfap-EGFP donorIntron targeting-mediated EGFP knockin at the zebrafish gfap locusgfap-EGFP (Danio rerio) Du
65565pT7-th-sgRNAin vitro trancription of sgRNA targeting the zebrafish th locusth sgRNA (Danio rerio) Du
65566pT7-gfap-sgRNAin vitro trancription of sgRNA targeting the zebrafish gfap locusgfap sgRNA (Danio rerio) Du
66593ins:CFP-NTRSpecific and conditional cell ablation of beta cells in the zebrafish pancreasInsulin promotor driving CFP-nitroreductase (Danio rerio) Stainier
67568DF-UAS-spa-GCaMP6fZebrafish expression of a photoactivatable fluorescent calcium reporterspa-GCaMP6f (Synthetic) Isacoff
67569DF-UAS-sspa-GCaMP6fZebrafish expression of a short photoactivatable fluorescent calcium reportersspa-GCaMP6f (Synthetic) Isacoff
67668Bact2-APEX2-GBP in pDEST-Tol2-pA2-acrys-cherryZebrafish expression vector for ubiquitous expression of APEX2-GBPAPEX2-GBP (Synthetic) Parton
68310pcDNA3.1 phiC31In vitro transcription vector to generate phiC31 mRNA (i.e. for zebrafish injection).phiC31 (Other) Basler
68313pDestattB (pCM268)Multisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafish Mosimann
68339pDestattB_ubi:EGFP (pCM272)Multisite Gateway destination vector for phiC31-mediated transgenesis in zebrafish, harbors ubiquitin promoter (ubi)-driven EGFP.EGFP (Other) Mosimann
68341pDestattB/CY (pCM327)Multisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafish, contains an attB site and alpha-cystallin:YFP (CY) transgenesis marker in backbone. Mosimann
69542pmtb-t7-alpha-bungarotoxinFor in vitro transcription of alpha-bungarotoxin mRNA that can be injected into embryos.alpha-bungarotoxin (Synthetic) Megason
70052p5E-mfap45' Entry vector containing the zebrafish mfap4 promoter. Used with Gateway Recombination to generate transgenic constructs exhibiting macrophage-restricted expression of transgenes.mfap4 promoter (Danio rerio) Tobin
71282HSP70l-APEX2-GBP in pDEST-Tol2-pA2-acrys-mCherryZebrafish expression vector for heat-shock inducible expression of APEX2-GBPAPEX2-GBP (Synthetic) Parton
72433pCS2+_Myr-mIFPExpression of Myr-mIFP in ZebrafishMry-mIFP Shu
72640p5E-elavl3Gateway p5E 5'entry clone with elavl3 (HuC) enhancer/promoter for pan-neuronal expression in zebrafishelavl3 enhancer (Danio rerio) Bonkowsky
73198pBS-TarHom-Tol2-FRT-GalK-cryaa-YFPBAC targeting cassette for pTARBAC2 with GalK selection marker and lens-specific transgenesis marker CitrineYFP (Synthetic) Knaut
73200pBS-TarHom-Tol2-FRT-GalK-cryaa-CFPBAC targeting cassette for pTARBAC2 with GalK selection marker and lens-specific transgenesis marker CeruleanCFP (Synthetic) Knaut
73202pBS-TarHom-Tol2-TetR-cryaa-YFPBAC targeting cassette for pTARBAC2 with tetR selection marker and lens-specific transgenesis marker CitrineYFP (Synthetic) Knaut
73203pBS-IndHom-Tol2-FRT-GalK-cryaa-dsRedBAC targeting cassette for pIndigoBAC-536 with GalK selection marker and lens-specific transgenesis marker dsReddsRed (Synthetic) Knaut
73204pBS-IndHom-Tol2-TetR-cryaa-dsRedBAC targeting cassette for pIndigoBAC-536 with tetR selection marker and lens-specific transgenesis marker dsReddsRed (Synthetic) Knaut
74009pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2Tissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoter5x UAS (Saccharomyces cerevisiae), Cas9 (Other), T2A, GFP, U6 promoter (Danio rerio) Del Bene
74010pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2Tissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoter5xUAS, Cas9, Cre, U6 promoter Del Bene
74116pCS2+ MBIC5-mCer3For generating mRNA for zebrafish injection of cytoplasmic fluorogen-activating protein (FAP)MBIC5-mCer3 (Synthetic) Tsang
74117pISceI-myl7:MBIC5-mCer3Zebrafish transgenic constructMBIC5-mCer3 (Synthetic) Tsang
74302pDestTol2CG2-Neurog1-Magneto2.0-p2A-mCherry-pAExpression of Magneto2.0-p2A-mCherry in Rohon-Beard sensory neurons of the zebrafishMagneto2.0 (Homo sapiens) Guler
7431414UAS zfPSD95:GFPA fusion of zebrafish PSD95 to GFP under control of 14 UAS repeatszebrafish PSD95 (Danio rerio) Smith
7431514UAS PSD95:GFP 5UAS DSRedExpressEncodes a PSD95:GFP fusion protein and DsRedExpress from separate UAS cassettes; for long-term two-photon time-lapse imaging of dendrite growth and synaptogenesis in zebrafishzebrafish PSD95 (Danio rerio), DsRedExpress (Other) Smith
743165UAS zfSynaptophysin:GFPA fusion of zebrafish synaptophysin to GFP under the control of 5UAS repeatssynaptophysin (Danio rerio) Smith
743175UAS zfSynaptophysin:GFP-5UAS DsRedExpressA fusion of zebrafish synaptophysin to GFP and DsRedExpress under the control of separate UAS cassettes; for dual-labeling of retinal ganglion cell (RGC) axon arbors in zebrafishsynaptophysin (Danio rerio), DsRedExpress (Other) Smith
74592pKHR4an ampR plasmid built from pBluescript: the multiple cloning site (mcs) flanked by inverted head-to-head oriented I-SceI sites. Genbank accession number is KU144822. Grunwald
74593pKHR5an alpha-crystallin::Venus (green lens) reporter cassette flanked by FRT sites and with a single loxP site at the 3’ end was inserted in the middle of pKHR4 mcs. Genbank accession number is KU144823.FRT-alpha-crystallin::Venus-FRT-loxP reporter cassette (Danio rerio) Grunwald
74624pKHR7a cmlc2::mCherry (red heart) reporter gene was inserted at the 3’end of pKHR4 mcs, that resides within donor fragments produced by I-SceI digestion. Genbank accession number is KU144824.cmlc2::mCherry (Danio rerio) Grunwald
74625pKHR8a plasmid derived from pKHR5: in addition to Venus reporter gene, it also contains cmlc2::mCherry (red heart) reporter gene at 3’ end of the mcs as pKHR7. Genbank accession number is KU144825.FRT-alpha-crystallin::Venus-FRT-loxP reporter cassette (Danio rerio), cmlc2::mCherry (Danio rerio) Grunwald
74632p5E--3mnx1Can be used to drive expression specifically in zebrafish motor neuronsmotor neuron and pancreas homeobox 1 promoter (Danio rerio) Cole
74736pDestTol2pA2-9xGREGRE-dependent EGFP reporter construct flanked by the minimal Tol2 transposon elementsEGFP (Other) Dalla Valle
78627pTol2-DRv7GESTALT V7 barcode for zebrafishGESTALT lineage tracing target V7 (Danio rerio) Shendure
78628pTol2-DRv6GESTALT V6 barcode for zebrafishGESTALT lineage tracing target V6 (Danio rerio) Shendure
80904pMT-HuC:Dendra2Injection into Zebrafish larvae together with Tol2-Transposase to achieve pan-neuronal expression of the photoconvertible fluorescent protein Dendra2Dendra2 (Synthetic) Pantazis
81234p5E-mitfa2.1zebrafish mitfa promoter entry vector for multisite Gateway cloningmitfa promoter (Danio rerio) Lister
82400p5E-elavl3Gateway-compatible zebrafish elavl3 promoterelavl3 promoter (Danio rerio) Eisen
82401p5E-gfapGateway-compatible zebrafish gfap promotergfap promoter (Danio rerio) Eisen
82402p5E-mnx1Gateway-compatible zebrafish mnx1 promotermnx1 promoter (Danio rerio) Eisen
82583p5E-EF1a/b-actin5' entry vector containing the EF1a/B-globin fused to zebrafish b-actin 2 enhancer/promoter for strong semi-ubiquitous expressionEF1a/b-globin fused to zebrafish b-actin 2 enhancer/promoter Stankunas
82584p5E-dusp65' entry vector containing FGF-responsive regulatory element of the zebrafish dusp6 promoter for FGF-responding cellsFGF-responsive element of the dusp6 promoter (Danio rerio) Stankunas
82585p5E-krt55' entry vector containing the zebrafish krt5 promoter for epidermis-specific expressionzebrafish krt5 promoter (Danio rerio) Stankunas
84032pHD066Vector for creating transgenic zebrafish. Expresses mCherry-Cre fusion protein under the elastase promoter and Venus under the crystalin promoter in zebrafishCre (Other), Venus (Other) Stainier
84033pHD157Vector for creating transgenic zebrafish. Expresses mCherry-Cre fusion protein under the fabp10 promoter and Venus under the crystalin promoter in zebrafishCre (Other), Venus (Other) Stainier
846038xGliBS-IVS2-mCherry-NLS-Odc1-polyA-Tol2Tol2-based hedgehog signal reporter plasmid for zebrafish transgenesismCherry (Synthetic) Chen
846048xGliBS-IVS2-mCherry-NLS-polyA-Tol2Tol2-based hedgehog signal reporter plasmid for zebrafish transgenesismCherry (Synthetic) Chen
85418Dat - EGFP - Tol2for making transgenic zebrafish expressing GFP under the control of cis-regulatory elements of the dopamine transporter (dat) genedopamine transporter (Danio rerio) Ekker
86248pTol-Promoter:NLS-tdMCP-eGFPFor generating transgenic lines that ubiquitously expressing the fluorescent MCP reporterbeta Actin promoter:NLS-tdMCPeGFP Marlow
86371pUAS-MmTFP13xmycmembrane targeted Tfp1 expression in zebrafishmTfp1 (Other) Wong
86372pUAS-MEYFPmembrane targeted EYFP expression in zebrafishEYFP (Other) Wong
86373pCx55.5-TrpRfor zebrafish transgenesis, expression of TrpR from Cx55.5 promoter, also expresses mCherryTrpR (Other) Wong
86374pME-nfsB-CFPexpression of nfsB-CFPnitroreductase (Other) Wong
86375pSws1-nfsB-CFPfor zebrafish transgenesis, expression of nfsB-CFP, also expresses EGFPnitroreductase (Other) Wong
89886pCrysβ:ECFP-LexOP:mCherry-NRasQ61KHuman NRasQ61K fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.mCherry-NRasQ61K (Homo sapiens) Sauka-Spengler
89887pCrysβ:ECFP-LexOP:mCherry-HrasG12VHuman HRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.mCherry-HRasG12V (Homo sapiens) Sauka-Spengler
89888pCrysβ:ECFP-LexOP:mCherry-KrasG12VHuman KRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.mCherry-KRasG12V (Homo sapiens) Sauka-Spengler
92388pTol2pA2-ubb:secHsa.ANNEXINV-mVenus, cryaa:RFPCell death reporterANNEXINV (Homo sapiens) Cole
92400PhOTO-N (pMTB:memb-Cerulean-2A-H2B-Dendra2)Constitutive expression of nuclear green-to-red photoconvertible Dendra2 and the membrane blue fluorescent protein Cerulean.Dendra2 (Synthetic), Cerulean (Synthetic) Pantazis
92401PhOTO-M (pMTB:memb-Dendra2-2A-H2B-Cerulean)Constitutive expression of membrane green-to-red photoconvertible Dendra2 and the nuclear blue fluorescent protein Cerulean.Dendra2 (Synthetic), Cerulean (Synthetic) Pantazis
92402PhOTO-C (pMTB:cyto-Dendra2-2A-H2B-Cerulean)Constitutive expression of cytosolic green-to-red photoconvertible Dendra2 and the nuclear blue fluorescent protein Cerulean.Dendra2 (Synthetic), Cerulean (Synthetic) Pantazis
101739pCMV-BE-zCas9expression of an optimized Cas9 for base-editing in zebrafishrAPOBEC1-XTEN-zCas9-UGI-NLS (Rattus norvegicus) Lin
101740pCMV-BE-VQRCas9expression of an optimized Cas9 for base-editing in zebrafishrAPOBEC1-XTEN-zCas9(VQR)-UGI-NLS (Rattus norvegicus) Lin
102417pVC-Ds-E1b:eGFP-DsFluorescent vector for screening enhancer elements in zebrafishE1b:eGFP (Danio rerio) Sauka-Spengler
105127pKE4-I-SceI-fabp10a:Xpt-β-cat, cryaa:VenusUsed along with I-SceI enzyme to generate transgenic zebrafish expressing hepatocyte-specific Xenopus activated beta-catenin and lens-specific Venus fluorescent proteinZebrafish fabp10a promoter:Xenopus pt-beta-catenin coding sequence, polyA (Xenopus laevis), Zebrafish cryaa promoter:Venus fluorescent protein coding sequence (Danio rerio) Stainier
105664Tol2-BetaActin-SecA5-YFPUbiquitously expresses a secreted and fluorescently tagged Annexin5 for in-vivo apoptosis reporterSecreted Annexin5-EYFP (Homo sapiens) Deng
106425Tol2-ngn1-Trpa1b-2A-mCherryFor expressing Trpa1b-2A-mCherry in zebrafish Rohon-Beard neurons.cmlc2 promoter (Danio rerio), Trpa1b (Danio rerio) Peterson
107185pT3TS-zAsCpf1plasmid to carry out IVT of AsCpf1 (zebrafish codon optimized)AsCpf1 (Danio rerio) Giraldez
107590Tol2-lyzC-mito-Dendra2Expresses mitochondria localized Dendra2 in zebrafish neutrophilsmito-Dendra2 (Danio rerio) Deng
108355UAS:bloSwitchUAS driven green to red switch reporter for B3 recombinase expressionUAS:[Ubiquitin-C-intron]-[blown-out site]-nuclear-GFP-[beta-globin-polyA]-[blown out site]-[membrane TagRFPT] (Synthetic) Burgess
108356UAS:iGluSnfr.zf1UAS driven variant of iGluSnFR for zebrafish expressionUAS:[GlyRa1 secretion sequence]-[iGluSnFR] (Synthetic) Burgess
108415pTol2-UAS-zmiRFPCodon-optimized monomeric near-infrared fluorescent protein miRFP in zebrafish expression plasmidzmiRFP (Synthetic) Boyden
108427pTol2-UAS-zArchon1-KGC-EGFP-ER2Codon-optimized Archon1 fluorescent voltage reporter in zebrafish expression plasmidzArchon1-KGC-EGFP-ER2 (Synthetic) Boyden
108428pTol2-UAS-zArchon2-KGC-EGFP-ER2Codon-optimized Archon2 fluorescent voltage reporter in zebrafish expression plasmidzArchon2-KGC-EGFP-ER2 (Synthetic) Boyden
108870pTol2-hspDRv7_scGstltscGESTALT V7 lineage barcode for zebrafishscGESTALT lineage tracing target V7 (Synthetic) Schier
108871pTol2-hsp70l:Cas9-t2A-GFP, 5xU6:sgRNAExpression of heat shock inducible Cas9-GFP and U6-driven 5 individual gRNAs for scGESTALT in zebrafish5xU6:sgRNA (Danio rerio), Cas9-t2A-GFP (Danio rerio) Schier
109490actc1b-mKate2-GPI-pAMuscle specific zebrafish expression of mKate2 fused to the 5' and 3' sequences from zebrafish folate receptor 1actc1b-mKate2-GPI-pA (Synthetic) Parton
109542pME-mKate2-GPI-pAMultisite gateway vector for expression of mKate2 fused to the 5' and 3' sequences from zebrafish folate receptor 1mKate2 fused to the 5' and 3' sequences from zebrafish folate receptor 1 (Synthetic) Parton
109768pT3TS_TransposaseCodon optimised transposase sequence in an optimal vector for in vitro transcription. Contains the T3 promoter and synthetic polyA tract.Tol2 Transposase (Synthetic) Parton
109769p3E-rev_U6_MCSMultisite gateway vector for 3' expression of a guide RNA from the U6 promoter (cassette in the reverse orientation). Contains BseRI sites for insertion of gRNA sequenceU6-gRNA cassette (Synthetic) Parton
110282ins:MCS; cryaa:RFPA zebrafish insulin promoter containing vector with multiple cloning sites. Contains red eye marker, and is flanked with I-SceI sites. Ninov
110283ins:BB1.0L; cryaa:RFPA zebrafish insulin promoter driving the Brainbow (BB1.0L) cassette. Contains red eye marker, and is flanked with I-SceI sites.Brainbow1.0L (Synthetic) Ninov
110285ins:GCaMP6s; cryaa:RFPA zebrafish insulin promoter driving the calcium indicator, GCaMP6s. Contains red eye marker, and is flanked with I-SceI sites.GCaMP6s (Other) Ninov
110286ins:MCS2; cryaa:RFPA zebrafish insulin promoter containing vector with a second multiple cloning sites. Contains red eye marker, and is flanked with I-SceI sites. Ninov
112205pBS-I-Sce1-eno2:Tau-IRES-egfpUsed to make transgenic zebrafish expressing human 4R/0N Tau under the neuronal eno2 promoterMAPT (Homo sapiens) Burton
112225pMTBExpression of membrane-tagged super folder GFP from zebrafish beta-actin promoter.Super-folder GFP (Other) Megason
115515pTol2pA2-mnx1:MLS-KillerRedPlasmid driving expression of membrane bound KillerRed fluorophore in zebrafish motor neuronsMLS-KillerRed (Other) Morsch
1155164xnrUAS:MLS-KillerRed in pTol2pA2-acrys-EGFP-Plasmid driving expression of membrane bound KillerRed fluorophore under UAS response element, with a green lens reporterMLS-KillerRed (Other) Morsch
115517pME-MLS-KillerRedMiddle entry plasmid containing membrane bound KillerRed fluorophoreMLS-KillerRed (Other) Morsch
118844MiniCoopR 2xU6:gRNA, mitfa:Cas9Targets 2 genes and expresses zebrafish mitfa specifically in zebrafish melanocytes Zon
119037pTol2-elavl3-VoltronPan neuronal expression of Voltron in zebrafishVoltron (Synthetic) Schreiter
119038pTol2-elavl3-Voltron-STPan neuronal expression of soma-localized Voltron in zebrafishVoltron-ST (Synthetic) Schreiter
119039pTol2-10xUAS-VoltronUAS-driven expresion of Voltron in zebrafishVoltron (Synthetic) Schreiter
119040pTol2-10xUAS-Voltron-STUAS-driven expresion of soma-localized Voltron in zebrafishVoltron-ST (Synthetic) Schreiter
119716pCS2(+)-zGradPlasmid to generate zGrad mRNA to inactivate proteins in zebrafishzGrad (Danio rerio) Knaut
121987pDestTol2-ubi-ASAP1ubiqutously express ASAP1 voltage biosensorASAP1 (Synthetic) Zhang
122129pTol2-elavl3_NES-rsCaMPARI-mRuby3Pan-neuronal expression of rsCaMPARI in zebrafish, nucleus excludedrsCaMPARI (Synthetic) Schreiter
124231pTol1-UAS:ChrimsonR-tdTomatoZebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChrimsonR fused to the red fluorescent protein tdTomatoChrimsonR-tdTomato (Other) Bianco
124232pTol1-UAS:Chronos-tdTomatoZebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin Chronos fused to the red fluorescent protein tdTomatoChronos-tdTomato (Other) Bianco
124233pTol1-UAS:CoChR-tdTomatoZebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CoChR fused to the red fluorescent protein tdTomatoCoChR-tdTomato (Other) Bianco
124234pTol1-UAS:CheRiff-tdTomatoZebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CheRiff fused to the red fluorescent protein tdTomatoCheRiff-tdTomato (Other) Bianco
124235pTol1-UAS:GtACR1-tdTomatoZebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin GtACR1 fused to the red fluorescent protein tdTomatoGtACR1-tdTomato (Other) Bianco
124236pTol1-UAS:GtACR2-tdTomatoZebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin GtACR2 fused to the red fluorescent protein tdTomatoGtACR2-tdTomato (Other) Bianco
124237pTol1-UAS:ChR2(H134R)-tdTomatoZebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChR2(H134R) fused to the red fluorescent protein tdTomatoChR2(H134R)-tdTomato (Other) Bianco
124431PCS2-FlipGFP(Casp3 cleavage seq) T2A mCherryExpresses FlipGFP (caspase-3 cleavage sequence) protease reporter and T2A mCherry in zebrafishFlipGFP(Casp3 cleavage seq) T2A mCherry (Synthetic) Shu
124432PCS2-FlipGFP(Casp3 cleavage seq)Expresses FlipGFP (caspase-3 cleavage sequence) protease reporter in zebrafish (verified)FlipGFP(Casp3 cleavage seq) (Synthetic) Shu
124433pCS2-GFP1-9Expresses GFP1-9 in zebrafishGFP1-9 (Synthetic) Shu
125690pT2-5xERE:GFPTransgene to report estrogen receptor activity in zebrafish5xERE:GFP (Synthetic) Halpern
125697pT2-bactin:QF2,crybb:CFPExpresses QF2 ubiquitously in zebrafish, with selectable marker CFP in eye lensbactin:QF2 (Danio rerio) Gorelick
127591pBT2-4xUAS:P2A-EGFPThis vector is used for cloning a gene driven by 4xnrUAS, to be co-expressed with EGFP through P2A self-cleaving peptides. Fang
127592pBT2-4xUAS:P2A-mCerulean3This vector is used for cloning a gene driven by 4xnrUAS, to be co-expressed with mCerulean3 through P2A self-cleaving peptides. Fang
127594pBT2-4xUAS:NICD-P2A-mRFPZebrafish NICD was driven by 4xnrUAS, to be co-expressed with mRFP through P2A self-cleaving peptides.NICD (Danio rerio) Fang
128838Tg(lyz:EGFP-Mmu.Rpl10a)RNA isolation from neutrophils via TRAPEGFP-L10a Huttenlocher
128839Tg(krt4:EGFP-Mmu.Rpl10a)RNA isolation from keratinocytes via TRAPEGFP-L10a Huttenlocher
128840Tg(mpeg1:EGFP-Mmu.Rpl10a)RNA isolation from machrophages via TRAPEGFP-L10a Huttenlocher
129265pTol2-Huc-Positron-STpan neuronal expression of soma-localized Positron in zebrafishPositron-ST (Synthetic) Schreiter
129266pT2-Tbait-UAS-Positron-STUAS-driven expression of Positron in zebrafishPositron-ST (Synthetic) Schreiter
129590pCMV-15F11-HA-mEGFPThe encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP) (Homo sapiens) Stasevich
129591pCMV-15F11-HA-mChThe encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.Anti-HA frankenbody-mCherry (15F11-HA scFv-mCh) (Homo sapiens) Stasevich
129592pCMV-15F11-HA-HaloThe encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the HaloTag. It can be used to track mature and nascent HA tagged proteins in living organism.Anti-HA frankenbody-HaloTag (15F11-HA scFv-HaloTag) (Homo sapiens) Stasevich
129595pCMV-2E2-HA-mEGFPThe encoded protein is the anti-HA frankenbody variant fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.Anti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP) (Homo sapiens) Stasevich
129596pCMV-2E2-HA-mChThe encoded protein is the anti-HA frankenbody variant fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.Anti-HA frankenbody variant-mCherry (2E2-HA scFv-mCherry) (Homo sapiens) Stasevich
131780pCS2+PIF6CreNMammalian, Zebrafish, or Xenopus expression of PIF6CreN, one half of the CreLite systemPIF6CreN (Arabidopsis thaliana) Eisenhoffer
131781pCS2+PhyBΔCreCMammalian, Zebrafish, or Xenopus expression of PhyBΔCreC, one half of the CreLite system.PhyBCreC (Arabidopsis thaliana) Eisenhoffer
131783pTol2-CreLiteTol2 destination vector carrying Ubi:CreLite with mTagBFP2 as a visual marker.PhyBCreC, PIF6CreN, and mTagBFP2 (Arabidopsis thaliana) Eisenhoffer
131787pTol2-PcyA-IRES-HO1Tol2 bicistronic vector expressing PcyA and HO1 for Phycocyanobilin (PCB) synthesis, driven by heatshock promoter hsp70.PcyA and HO1 (Homo sapiens) Eisenhoffer
135214pDEST mfap4:tdTomatoRed tagged macrophagesmfap4 promoter and tdTomato (Danio rerio) Tobin
135215pDEST mpeg1:tomato-CAAXRed tagged macrophagesmpeg1 and tdTomato (Danio rerio) Tobin
135217pDEST mfap4:icre-p2a-tomatoCre coexpressed with tdTomato in macrophagesmfap4 promoter with iCre and Tomato (Danio rerio) Tobin
135218pDEST mfap4:turquoise2Cyan tagged macrophagesmfap4 promoter and mTurquoise2 (Danio rerio) Tobin
137185pTol2-HuC(elavl3)-CaMPARI2pan-neuronal expression of CaMPARI2 in zebrafish, used to generate the CaMPARI2 zebrafish line at ZIRC (ZL13801)HuC(elavl3)-CaMPARI2-FLAG-HA-myc-polyA (Rattus norvegicus) Schreiter
154911pCS2+-Y276H PHYB-CAAX1-621 amino acids of Arabidopsis PHYB (as in construct Addgene ID 154910) with the CAAX membrane moiety tag. Tyrosine residue 276 of PHYB was mutated to Histidine. Phytochrome B (Arabidopsis thaliana) Clarke
154913pCS2+-PIF6-EGFP1-100 amino acids of Arabidopsis PIF6 (Gene ID: 825382), tagged with EGFP fluorescent protein. Mammalian codon optimised.phytochrome interacting factor 3-like 2 (Arabidopsis thaliana) Clarke
154914pCS2+-Pard3-EGFP-PIF6Full length zebrafish Pard3 (Gene ID: 403050), tagged with EGFP fluorescent protein and Arabidopsis PIF6 (as in construct Addgene ID 154913).par-3 family cell polarity regulator alpha, b (Danio rerio) Clarke
155119pTol2CG2-ubi:QFGal4-SV40pATol2 vector for ubiquitous expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis markerubi:QFGal4 (Danio rerio) Pearson
155120pTol2CG2-hsp70I:QFGal4-SV40pATol2 vector for heat-shock inducible expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis markerhsp70l:QFGal4 (Danio rerio) Pearson
155121pTol2CG2-acta1:QFGal4-SV40pATol2 vector for skeletal-muscle expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis markeracta1:QFGal4 (Danio rerio) Pearson
155122pTol2CG2-QUAS5x:GFPNLS-SV40pATol2 vector containing QUAS5x reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis markerQUAS5x:GFPNLS (Synthetic) Pearson
155123pTol2CG2-QUASM1:GFPNLS-SV40pATol2 vector containing QUASM1 reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis markerQUASM1:GFPNLS (Synthetic) Pearson
155124pTol2CG2-QUASM1:mScarletNLS-SV40pATol2 vector containing QUASM1 reporter element upstream of mScarlet-NLS. Includes cmlc2:mRFP-SV40pA transgenesis markerQUASM1:mScarletNLS (Synthetic) Pearson
155125pTol2-ubi:loxP-mScarletNLS-SV40pA-loxp-QFGal4-SV40pATol2 vector containing Cre-inducible QFGal4-switchQFGal4-switch (Saccharomyces cerevisiae) Pearson
155126pCS2-QFGal4-SV40pAIn vitro transcription of QFGal4 mRNAQFGal4 (Saccharomyces cerevisiae) Pearson
1586515xUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2making transgenics expressing a UAS driven YFP and NTR 2.05xUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y;he:BFP (Other) Mumm
1586525xUAS:GAP-EGFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2making transgenics expressing a UAS driven eGFP and NTR 2.05xUAS:GAP-eGFP-P2A-NfsB_Vv F70A/F108Y;he:BFP (Other) Mumm
1586535xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2making transgenics expressing a UAS driven mCherry and NTR 2.05xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y;he:BFP (Other) Mumm
1586545xQUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y,he:ECFPmaking transgenics expressing a QUAS driven YFP and NTR 2.05xQUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y;he:eCFP (Other) Mumm

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Highlights of Zebrafish Tools in our Collection

CRISPR

Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology or the zebrafish CRISPR resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of useful zebrafish CRISPR resources can be found below:

Lineage Tracing

Cell lineage tracing in multicellular systems typically relies on genetic markers, somatic mutations, or labeling with fluorescent proteins to visualize and track clones of cells over time. Examples of useful tools for studying cells that share clonal origin in zebrafish can be found below:

Other Tools of Interest

A few examples of popular tools to use in zebrafish, ranging from optogenetics to tools for studying diseases, are listed below:

  • NTR 2.0 - Jeff Mumm Lab. NTR 2.0/MTZ-based cell ablation system that allows sustained cell loss and ablation of previously resistant cell types.
  • CreLite - George Eisenhoffer Lab. An optogenetically controlled Cre/loxP system that enables precise temporal and spatial control of gene expression in zebrafish using red light.
  • LipoGlo - Steven Farber Lab. A reporter system that allows direct assessment of lipoprotein abundance, size, and localization in high-throughput.
  • MAZERATI platform - Leonard Zon Lab. A platform to rapidly model potential cancer drivers in vivo.
  • Biotagging toolkit - Tatjana Sauka-Spengler Lab. A genetic binary system for cell- and compartment-specific biotin labeling in zebrafish.
  • pGCS Vector Kit - Hai-Ning Du Lab. A set of pCS2+ backbone-based Gateway destination vectors (pGCS), bearing either amino- or carboxyl-terminal tags, including Myc, HA, Flag, GST and eGFP epitopes.
  • Zebrafish Tol2 Gateway-compatible Kit for Neurodegenerative Disorders - Nicholas Cole Lab. A kit consisting of plasmids with four promoters, six fluorophores, and empty vectors designed to streamline the production of transgenic zebrafish for studying neurodegenerative disorders.
  • Fluorescent Protein Fusion Construct Set - Amro Hamdoun Lab. A useful “starter kit” for screening fluorescent protein fusion expression in organisms or cells in which an exogenous mRNA can be injected and expressed.
  • EXPRESs Plasmids for Zebrafish Receptors and Zebrafish Monoclonal Antibody plasmids - Gavin Wright Lab. Collections of plasmids that encode zebrafish receptors or monoclonal antibodies to zebrafish cell surface receptors and secreted proteins.

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Other Resources

Addgene Blog Posts

External Resources

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Do you have suggestions for other plasmids that should be added to this list?

Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource!