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Re-purposing Ac/Ds transgenic system for CRISPR/dCas9 modulation of enhancers and non-coding RNAs in zebrafish
Chong-Morrison V, Simões FC, Senanayake U, Carroll DS, Riley PR, Sauka-Spengler T
bioRxiv
Article

Plasmids from Article

ID Plasmid Purpose  
102416pVC-Ds-MCS-DsEmpty vector with a multiple cloning site flanked by Ds integration elements
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102417pVC-Ds-E1b:eGFP-DsFluorescent vector for screening enhancer elements in zebrafish
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102418pAC-SP6Ac transposase with SP6 promoter for in vitro transcription
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119059pVC-Ds-Pax3a_enh5-E1b:eGFP-DsFluorescent vector with zebrafish pax3a enhancer 5 (strong activity)
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119061pVC-Tol2-Pax3a_enh5-E1b:eGFP-Tol2Fluorescent vector with zebrafish pax3a enhancer 5; Tol2 version
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119065pGEM-T-Easy-HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRTDonor construct to amplify HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombination
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119067pGEM-T-Easy-NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRTDonor construct to amplify NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombination
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119069pVC-Ds-DrU6a:sgRNA-DsTo clone sgRNA spacer sequence for microinjections in zebrafish
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119070pVC-Ds-DrU6a:scrambled1-sgRNA-DsConstruct with scrambled-1 sgRNA sequence for microinjections in zebrafish
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119071pVC-Ds-DrU6a:scrambled2-sgRNA-DsConstruct with scrambled-2 sgRNA sequence for microinjections in zebrafish
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119072pVC-Ds-DrU6a:scrambled3-sgRNA-DsConstruct with scrambled-3 sgRNA sequence for microinjections in zebrafish
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119073pVC-Ds-DrU6a:scrambled4-sgRNA-DsConstruct with scrambled-4 sgRNA sequence for microinjections in zebrafish
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119074pVC-Ds-ubb:MCP-p65-HSF1-2a-Cerulean-DsZebrafish ubiquitin promoter driving MCP-p65-HSF1 with Cerulean reporter
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119075pVC-Ds-ubb:Com-KRAB-2a-Cerulean-DsZebrafish ubiquitin promoter driving Com-KRAB with Cerulean reporter
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119076pVC-Ds-DrU6a:sgRNA-MS2-DsTo clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x MS2 stem loops
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119077pVC-Ds-DrU6a:sgRNA-com-DsTo clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x com stem loops
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