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pAC-SP6
(Plasmid #102418)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 102418 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    p64T
  • Backbone size w/o insert (bp) 3500
  • Total vector size (bp) 5500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ac transposase
  • Species
    Maize
  • Insert Size (bp)
    2151
  • Promoter SP6

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Unmodified plasmid obtained from authors listed below via MTA dated 27th August 2012.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

A. Emelyanov and S. Parinov. Mifepristone-inducible LexPR system to drive and control gene expression in transgenic zebrafish. Developmental Biology, 320(1):113– 121, 2008. ISSN 00121606. doi: 10.1016/j.ydbio.2008.04.042.

A. Emelyanov, Y. Gao, N. I. Naqvi, and S. Parinov. Trans-kingdom transposition of the maize Dissociation element. Genetics, 174(3):1095–1104, 2006. ISSN 00166731. doi: 10.1534/genetics.106.061184.

Usage notes: Linearise with BamHI for in vitro transcription.

Note: The AC transposase insert covers amino acids 103 through 807.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAC-SP6 was a gift from Tatjana Sauka-Spengler (Addgene plasmid # 102418 ; http://n2t.net/addgene:102418 ; RRID:Addgene_102418)
  • For your References section:

    Re-purposing Ac/Ds transgenic system for CRISPR/dCas9 modulation of enhancers and non-coding RNAs in zebrafish. Chong-Morrison V, Simões FC, Senanayake U, Carroll DS, Riley PR, Sauka-Spengler T. bioRxiv 10.1101/450684