|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||102418||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3500
- Total vector size (bp) 5500
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameAc transposase
Insert Size (bp)2151
- Promoter SP6
- Cloning method Unknown
- 5′ sequencing primer atttaggtgacactataga (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byUnmodified plasmid obtained from authors listed below via MTA dated 27th August 2012.
Terms and Licenses
- Not Available to Industry
A. Emelyanov and S. Parinov. Mifepristone-inducible LexPR system to drive and control gene expression in transgenic zebrafish. Developmental Biology, 320(1):113– 121, 2008. ISSN 00121606. doi: 10.1016/j.ydbio.2008.04.042.
A. Emelyanov, Y. Gao, N. I. Naqvi, and S. Parinov. Trans-kingdom transposition of the maize Dissociation element. Genetics, 174(3):1095–1104, 2006. ISSN 00166731. doi: 10.1534/genetics.106.061184.
Usage notes: Linearise with BamHI for in vitro transcription.
Note: The AC transposase insert covers amino acids 103 through 807.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAC-SP6 was a gift from Tatjana Sauka-Spengler (Addgene plasmid # 102418 ; http://n2t.net/addgene:102418 ; RRID:Addgene_102418)
For your References section:Re-purposing Ac/Ds transgenic system for CRISPR/dCas9 modulation of enhancers and non-coding RNAs in zebrafish. Chong-Morrison V, Simões FC, Senanayake U, Carroll DS, Riley PR, Sauka-Spengler T. bioRxiv 10.1101/450684
Map uploaded by the depositor.