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viral-icon-01.png Viral Service: AAV


We are offering ready-to-use viral preparations of AAV plasmids in our repository. With viral service, Addgene provides high-quality and high-titer viral preparations. We perform quality control experiments, including AAV titering by SYBR green qPCR, confirmation of the transfer plasmid with PCR, purity assessment by silver staining, in vitro and (when possible) in vivo testing, and full sequencing of the final viral preparation. Results for our quality control experiments can be found on the material pages for viral catalog items. For descriptions of Addgene's viral production protocols and quality controls, visit our viral production page. For protocols on how to use virus from Addgene or how to make your own virus, see our list of viral protocols with tips and sample data.

Addgene's AAV are made from plasmids that are currently in our repository, so feel free to obtain the plasmid from us, or take advantage of our viral service by browsing our available AAV below!

Virus associated DNA

Each viral service request will also include virus associated DNA, which is a sample of the purified DNA from the original plasmid that was used to make the virus. In order for Addgene to provide viral services to you, we must ensure that you have the right to use the original plasmid.

Distribution of the original plasmid is important because it is the actual material being referenced in the Material Transfer Agreement (MTA) and its inclusion is what gives you permission to use the plasmid.

Browse Available AAV by Category

Chemogenetics (including DREADD) AAV

Use our chemogenetics viruses to chemically induce neuronal activity in specific cell types.

ID Name Promoter Receptor Integration Activity Serotype(s) PI
44361 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Syn1 hM3D(Gq) Cre-dependent Induces neuronal burst firing 2, 5, 8 Roth
44362 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Syn1 hM4D(Gi) Cre-dependent Induces neuronal silencing 2, 5, 8, rg* Roth
50459 pAAV-hSyn-DIO-mCherry Syn1 none Cre-dependent Control 2, 5, 8 Roth
50474 pAAV-hSyn-hM3D(Gq)-mCherry Syn1 hM3D(Gq) Non-cre dependent Induces neuronal burst firing 2, 5, 8 Roth
50475 pAAV-hSyn-hM4D(Gi)-mCherry Syn1 hM4D(Gi) Non-cre dependent Induces neuronal silencing 2, 5, 8 Roth
50465 pAAV-hSyn-EGFP Syn1 none Non-cre dependent Control 2, 5, 8 Roth
50476 pAAV-CaMKIIa-hM3D(Gq)-mCherry CaMKIIa hM3D(Gq) Non-cre dependent Induces neuronal burst firing 5, 8 Roth
50477 pAAV-CaMKIIa-hM4D(Gi)-mCherry CaMKIIa hM4D(Gi) Non-cre dependent Induces neuronal silencing 5, 8 Roth
50469 pAAV-CaMKIIa-EGFP CaMKIIa none Non-cre dependent Control 5, 8 Roth
50460 pAAV-EF1a-DIO-hM3D(Gq)-mCherry EF1a hM3D(Gq) Cre-dependent Induces neuronal burst firing 5 Roth
50462 pAAV-EF1a-DIO-mCherry EF1a none Cre-dependent Control 5 Roth
44361 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Coming Soon rg (retrograde)* Roth
50474 pAAV-hSyn-hM3D(Gq)-mCherry Coming Soon rg (retrograde)* Roth
50475 pAAV-hSyn-hM4D(Gi)-mCherry Coming Soon rg (retrograde)* Roth

*rg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova and Schaffer labs

Retrograde AAV (for targeting projection neurons)

The AAV retrograde serotype is produced with the AAV retro helper plasmid. This serotype permits robust retrograde access and transgene delivery to projection neurons. See our inventory below, or visit our AAV Service Page for Retrograde AAV.

ID Name Promoter Activity Serotype PI
Controls/Reporters
37825 AAV-CAG-GFP CAG GFP expression control rg (retrograde)* Boyden
59462 pAAV-CAG-tdTomato CAG tdTomato expression control rg (retrograde)* Boyden
51503 AAV pCAG-FLEX-tdTomato-WPRE CAG Cre-dependent tdTomato rg (retrograde)* Zeng
37120 pAAV-Ef1a-DO_DIO-TdTomato_EGFP-WPRE-pA EF1a Cre-dependent color-flipping reporter. Expresses tdTomato in the absence of Cre, but inverts to express EGFP in Cre-positive cells. rg (retrograde)* Sabatini
Site-Specific Recombinases
51507 AAV pmSyn1-EBFP-Cre Syn1 BFP-tagged Cre recombinase rg (retrograde)* Zeng
55634 New! pAAV-EF1a-mCherry-IRES-Flpo EF1a Flpo and physically-separate mCherry rg (retrograde)* Deisseroth
24593 AAV-pgk-Cre Coming Soon rg (retrograde)* Aebischer
Calcium Sensors
51085 AAV-hSyn1-GCaMP6f-P2A-nls-dTomato Syn1 Calcium sensor and physically separate nuclear dTomato rg (retrograde)* Ting
51083 AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato EF1a Cre-dependent calcium sensor and physically separate nuclear dTomato rg (retrograde)* Ting
51084 AAV-hSyn1-GCaMP6s-P2A-nls-dTomato Syn1 Calcium sensor and physically separate nuclear dTomato rg (retrograde)* Ting
Optogenetics
28017 AAV-CAG-hChR2-H134R-tdTomato CAG Channelrhodopsin/Excitatory rg (retrograde)* Svoboda
58880 pAAV-Syn-ChR2(H134R)-GFP Syn1 Channelrhodopsin/Excitatory rg (retrograde)* Boyden
20297 pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA Coming Soon rg (retrograde)* Deisseroth
65014 pAAV-hsyn-Jaws-KGC-GFP-ER2 Coming Soon rg (retrograde)* Boyden
84445 pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] Coming Soon rg (retrograde)* Boyden
Chemogenetics, DREADDs
44362 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Syn1 Chemogenetics/DREADD rg (retrograde)* Roth
44361 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Coming Soon rg (retrograde)* Roth
50474 pAAV-hSyn-hM3D(Gq)-mCherry Coming Soon rg (retrograde)* Roth
50475 pAAV-hSyn-hM4D(Gi)-mCherry Coming Soon rg (retrograde)* Roth

*rg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova and Schaffer labs

Optogenetics AAV

Optogenetic tools can be used to control cell activity in living tissue with light. Optogenetics is typically used in neuroscience to control electrical potentials in neurons. See our Optogenetics Plasmids page for more information about this field.

ID Name Opsin/Variant Activity Serotype PI
Activators
28017 AAV-CAG-hChR2-H134R-tdTomato Channelrhodopsin/ H134R mutant Light–drivable depolarization, larger photocurrents but slower channel kinetics than wild-type ChR2. Fused to tdTomato. rg (retrograde)* Svoboda
58880 pAAV-Syn-ChR2(H134R)-GFP Channelrhodopsin/ H134R mutant Light–drivable depolarization, larger photocurrents but slower channel kinetics than wild-type ChR2. Fused to GFP. rg (retrograde)* Boyden
59170 New! pAAV-Syn-Chronos-GFP Channelrhodopsin/Chronos Light–drivable depolarization 5 Boyden
62723 New! pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] Channelrhodopsin/ChrimsonR Cre-dependent, light–drivable depolarization 5 Boyden
20297 pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA Coming Soon rg (retrograde)* Deisseroth
Inhibitors
65014 pAAV-hsyn-Jaws-KGC-GFP-ER2 Coming Soon rg (retrograde)* Boyden
84445 pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] Coming Soon rg (retrograde)* Boyden

*rg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova and Schaffer labs

Serotype Testing Control AAV

Use our control AAV constructs to see which serotype works best for your study. Browse our viruses that express fluorescent proteins under general promoters and compare the activity of viruses with different serotypes.

ID Name Localization Promoter Activity Serotype PI
37825 pAAV-CAG-GFP General CAG GFP expression control rg (retrograde)* Boyden
59462 pAAV-CAG-tdTomato General CAG tdTomato expression control 5, rg (retrograde)* Boyden

*rg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova and Schaffer labs

AAV expressing site-specific recombinases

These AAV encode site-specific recombinases, which can be used to control gene expression.

ID Name Promoter Tag Serotype PI
Cre
51507 AAV pmSyn1-EBFP-Cre Syn1 EBFP 1, rg (retrograde)* Zeng
24593
AAV-pgk-Cre Coming Soon rg (retrograde)* Aebischer
Flpo
55634
New!
pAAV-EF1a-mCherry-IRES-Flpo EF1a physically-separate mCherry (not a fusion tag) rg (retrograde)* Deisseroth

*rg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova and Schaffer labs

Don’t See What You’re Looking For?

Please let us know what you would like to see available in viral format using the form below. Please note this does not guarantee viral service, but lets us know what viruses would be helpful to the scientific community.