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viral-icon-01.png Viral Service: AAV


We are offering ready-to-use viral preparations of AAV plasmids in our repository. With viral service, Addgene provides high-quality and high-titer viral preparations. Addgene's AAV are made from plasmids that are currently in our repository. You can obtain the plasmid from us or receive a ready-to-use viral preparation from the inventory below.

Browse Available AAV

By Function

By Serotype

  • Retrograde AAV (to target projection neurons)
  • AAV1, AAV2, AAV5, AAV8 are listed in the tables below

Chemogenetics (including DREADD) AAV

Use our chemogenetics AAV to chemically induce neuronal activity in specific cell types. See our Chemogenetics Plasmids and Chemogenetics Guide pages for more information about this field.

ID Name Receptor Activity Serotype(s) PI
Syn promoter, Cre-dependent
44361 pAAV-hSyn-DIO-hM3D(Gq)-mCherry hM3D(Gq) Activation 2, 5, 8 Roth
44362 pAAV-hSyn-DIO-hM4D(Gi)-mCherry hM4D(Gi) Inhibition 2, 5, 8, rg* Roth
50459 pAAV-hSyn-DIO-mCherry none Control 2, 5, 8 Roth
44361 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Coming Soon rg* Roth
65417 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine Coming Soon 8 Roth
Syn promoter, Non-cre-dependent
50474 pAAV-hSyn-hM3D(Gq)-mCherry hM3D(Gq) Activation 2, 5, 8 Roth
50475
New!
pAAV-hSyn-hM4D(Gi)-mCherry hM4D(Gi) Inhibition 2, 5, 8, rg* Roth
50465 pAAV-hSyn-EGFP none Control 2, 5, 8 Roth
50474 pAAV-hSyn-hM3D(Gq)-mCherry Coming Soon rg* Roth
CaMKIIa promoter, Non-cre-dependent
50476 pAAV-CaMKIIa-hM3D(Gq)-mCherry hM3D(Gq) Activation 5, 8 Roth
50477 pAAV-CaMKIIa-hM4D(Gi)-mCherry hM4D(Gi) Inhibition 5, 8 Roth
50469 pAAV-CaMKIIa-EGFP none Control 5, 8 Roth
EF1a promoter, Cre-dependent
50460 pAAV-EF1a-DIO-hM3D(Gq)-mCherry hM3D(Gq) Activation 5 Roth
50462 pAAV-EF1a-DIO-mCherry none Control 5 Roth

*rg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova and Schaffer labs

Optogenetics AAV

Optogenetic tools can be used to control cell activity in living tissue with light. Optogenetics is typically used in neuroscience to control electrical potentials in neurons. See our Optogenetics Plasmids page for more information about this field.

ID Name Opsin/Variant Activity Serotype PI
Activators
20297 New! pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA Channelrhodopsin/ H134R mutant Light–drivable depolarization. Fused to tdTomato. Cre-dependent. rg* Deisseroth
28017 AAV-CAG-hChR2-H134R-tdTomato Channelrhodopsin/ H134R mutant Light–drivable depolarization. Fused to tdTomato. rg* Svoboda
58880 pAAV-Syn-ChR2(H134R)-GFP Channelrhodopsin/ H134R mutant Light–drivable depolarization. Fused to GFP. rg* Boyden
59170 pAAV-Syn-Chronos-GFP Channelrhodopsin/Chronos Light–drivable depolarization. 5 Boyden
62723 pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] Channelrhodopsin/ChrimsonR Light–drivable depolarization. Fused to tdTomato. Cre-dependent. 5 Boyden
20298 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA Coming Soon 1 Deisseroth
58880 pAAV-Syn-ChR2(H134R)-GFP Coming Soon 8 Boyden
Inhibitors
65014
New!
pAAV-hsyn-Jaws-KGC-GFP-ER2 Halorhodopsin/Jaws Red-shifted, light-driven inward chloride pump, used to silence neuronal activity. rg* Boyden
84445 pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] Coming Soon rg* Boyden
65014 pAAV-hsyn-Jaws-KGC-GFP-ER2 Coming Soon 8 Boyden

*rg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova and Schaffer labs

Site-specific recombinases

These AAV encode site-specific recombinases, which can be used to control gene expression.

ID Name Promoter Tag Serotype PI
Cre
51507 AAV pmSyn1-EBFP-Cre Syn1 EBFP 1, rg* Zeng
24593 AAV-pgk-Cre PGK none rg* Aebischer
55632
New!
pAAV-Ef1a-mCherry-IRES-Cre EF1a physically-separate mCherry (not a fusion tag) rg* Deisseroth
Flpo
55634 pAAV-EF1a-mCherry-IRES-Flpo EF1a physically-separate mCherry (not a fusion tag) rg* Deisseroth

Calcium Sensors

ID Name Promoter Activity Serotype PI
GCaMP6f
51085 AAV-hSyn1-GCaMP6f-P2A-nls-dTomato Syn1 Calcium sensor and physically separate nuclear dTomato rg* Ting
51083 AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato EF1a Cre-dependent calcium sensor and physically separate nuclear dTomato rg* Ting
51085 AAV-hSyn1-GCaMP6f-P2A-nls-dTomato Coming Soon 1 Ting
GCaMP6s
51084
New!
AAV-hSyn1-GCaMP6s-P2A-nls-dTomato Syn1 Calcium sensor and physically separate nuclear dTomato 1, rg* Ting
51082 AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato Coming Soon 1 Ting

*rg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova and Schaffer labs

Controls/Reporters

Browse our control AAV that express fluorescent proteins under general promoters. These AAV can be used to compare the activity of different serotypes.

ID Name Promoter Activity Serotype PI
Non-cre-dependent
37825 AAV-CAG-GFP CAG GFP expression rg* Boyden
50465 pAAV-hSyn-EGFP hSyn EGFP expression 2, 5, 8 Roth
50469 pAAV-CaMKIIa-EGFP CaMKIIa EGFP expression 5, 8 Roth
59462 pAAV-CAG-tdTomato CAG tdTomato expression 5, rg* Boyden
Cre-dependent
37120 pAAV-Ef1a-DO_DIO-TdTomato_EGFP-WPRE-pA EF1a Cre-dependent color-flipping reporter. Expresses tdTomato in the absence of Cre, but inverts to express EGFP in Cre-positive cells. rg* Sabatini
50459 pAAV-hSyn-DIO-mCherry hSyn Cre-dependent mCherry expression 2, 5, 8 Roth
50462 pAAV-EF1a-DIO-mCherry EF1a Cre-dependent mCherry expression 5 Roth
51503 AAV pCAG-FLEX-tdTomato-WPRE CAG Cre-dependent tdTomato expression rg* Zeng

*rg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova and Schaffer labs


Virus associated DNA

Each viral service request will also include virus associated DNA, which is a sample of the purified DNA from the original plasmid that was used to make the virus. In order for Addgene to provide viral services to you, we must ensure that you have the right to use the original plasmid.

Distribution of the original plasmid is important because it is the actual material being referenced in the Material Transfer Agreement (MTA) and its inclusion is what gives you permission to use the plasmid.

Don’t See What You’re Looking For?

Please let us know what you would like to see available in viral format using the form below. Please note this does not guarantee viral service, but lets us know what viruses would be helpful to the scientific community.