Viral Service: Retrograde AAV

As part of our Viral Service, Addgene is producing ready-to-use viral vector preparations in a retrograde serotype from the Alla Karpova and David Schaffer laboratories. These viral vector preparations were produced with the rAAV2-retro helper plasmid (Addgene #81070). This serotype permits robust retrograde access and transgene delivery to projection neurons. Specific classes of projection neurons can be targeted by using this serotype to deliver Cre-dependent transgenes in Cre transgenic lines.

As part of our standard viral production, these viral vectors undergo quality control, including AAV titering by SYBR green qPCR, confirmation of the transfer plasmid with PCR, purity assessment by silver staining, in vitro and (when possible) in vivo testing, and full sequencing of the final viral vector preparation. In some cases, in vivo testing of these viral preparations was performed by the Karpova lab. Results for our quality control experiments can be found on the material pages for viral catalog items.

Browse Available Retrograde AAV

See our retrograde AAV based on the functional catagories listed below. Narrow down the items available within a category by using the buttons.

• Controls
• Green
• Red
• Switch
• Other

• Recombinases
• Cre
• Flp

• Calcium Sensors
• GCaMP7f
• GCaMP7s
• GCaMP7b
• GCaMP6f
• GCaMP6s
• GECO

• Other Biosensors
• Glutamate
• Norepinephrine
• Dopamine

• Optogenetics
• Activators
• Inhibitors

• DREADDs
• Activators
• Inhibitors

Clear Filters

ID	Name	Promoter	Activity	Category	PI
Controls
37825	AAV-CAG-GFP	CAG	GFP	Control	Boyden
50465	pAAV-hSyn-EGFP	Syn	EGFP	Control	Roth
114472	pAAV-hSyn-mCherry	Syn	mCherry	Control	Deisseroth
50459	pAAV-hSyn-DIO-mCherry	Syn	mCherry	Control	Roth
59462	pAAV-CAG-tdTomato	CAG	tdTomato	Control	Boyden
50457	pAAV-hSyn-DIO-EGFP	Syn	EGFP, Cre-dependent	Control	Roth
28306	pAAV-FLEX-tdTomato	CAG	tdTomato, Cre-dependent	Control	Boyden
27056	pAAV-Ef1a-DIO EYFP	EF1a	EYFP, Cre-dependent	Control	Deisseroth
99130	pAAV-mDlx-NLS-mRuby2	Dlx	NLS-mRuby2	Control	Gradinaru
83895	pAAV-hDlx-Flex-GFP-Fishell_6	Dlx	GFP	Control	Fishell
112677	pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP	EF1a	Color-flipping switch. Expresses NLS-mCherry in the absence of Cre, and expresses NLS-EGFP in Cre-positive cells.	Control	Harvey
Recombinases
24593	AAV-pgk-Cre	PGK	Cre expression	Recombinases	Aebischer
55632	pAAV-Ef1a-mCherry-IRES-Cre	EF1a	Cre and physically-separate mCherry expression	Recombinases	Deisseroth
105540	pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40	Syn	EGFP-tagged Cre expression	Recombinases	Wilson
105553	pENN.AAV.hSyn.Cre.WPRE.hGH	Syn	Cre expression	Recombinases	Wilson
55634	pAAV-EF1a-mCherry-IRES-Flpo	EF1a	Flpo and physically-separate mCherry expression	Recombinases	Deisseroth
55637 New!	pAAV-EF1a-Flpo	EF1a	Flpo	Recombinases	Deisseroth
121675 New!	pAAV-EF1a-fDIO-Cre	EF1a	Flp-dependent Cre expression	Recombinases	Engel, Nectow
Calcium Sensors
51085	AAV-hSyn1-GCaMP6f-P2A-nls-dTomato	Syn	GCaMP6f and physically separate nuclear dTomato	Calcium sensor	Ting
51083	AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato	EF1a	GCaMP6f and physically separate nuclear dTomato, Cre-dependent	Calcium sensor	Ting
51084	AAV-hSyn1-GCaMP6s-P2A-nls-dTomato	Syn	GCaMP6s and physically separate nuclear dTomato	Calcium sensor	Ting
100853	pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40	Syn	Cre-dependent jRGECO1a	Calcium Sensor	Kim, GENIE
100854	pAAV.Syn.NES-jRGECO1a.WPRE.SV40	Syn	jRGECO1a	Calcium Sensor	Kim, GENIE
104487	pGP-AAV-syn-jGCaMP7s-WPRE	Syn	GCaMP7s	Calcium sensor	Kim
104489	pGP-AAV-syn-jGCaMP7b-WPRE	Syn	GCaMP7b	Calcium sensor	Kim
104491	pGP-AAV-syn-FLEX-jGCaMP7s-WPRE	Syn	GCaMP7s, Cre-dependent	Calcium sensor	Kim
104493	pGP-AAV-syn-FLEX-jGCaMP7b-WPRE	Syn	GCaMP7b, Cre-dependent	Calcium sensor	Kim
104495 New!	pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE	CAG	GCaMP7s, Cre-dependent	Calcium sensor	Kim
104496	pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE	CAG	GCaMP7f, Cre-dependent	Calcium sensor	Kim
104488	pGP-AAV-syn-jGCaMP7f-WPRE	Syn	GCaMP7f	Calcium sensor	Kim
104492	pGP-AAV-syn-FLEX-jGCaMP7f-WPRE	Syn	GCaMP7f, Cre-dependent	Calcium sensor	Kim
Other Biosensors
106174	pAAV.hSynapsin.SF-iGluSnFR.A184S	Syn	Tight affinity glutamate sensor	Glutamate sensor	Looger
113050	pAAV-hSyn-GRAB_DA1h	Syn	genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h	Dopamine sensor	Li
123308	pAAV-hSyn-GRAB_NE1m	Syn	genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m	Norepinephrine sensor	Li
Optogenetics
20297	pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA	EF1a	Activator, Cre-dependent	Optogenetics	Deisseroth
20298	pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA	EF1a	Activator, Cre-dependent	Optogenetics	Deisseroth
26973	pAAV-hSyn-hChR2(H134R)-EYFP	Syn	Activator	Optogenetics	Deisseroth
28017	AAV-CAG-hChR2-H134R-tdTomato	CAG	Activator	Optogenetics	Svoboda
26975	pAAV-CaMKIIa-hChR2(H134R)-mCherry	CamKII	Activator	Optogenetics	Deisseroth
55645	pAAV-hSyn Con/Fon hChR2(H134R)-EYFP	Syn	Activator, Cre and Flp-dependent	Optogenetics	Deisseroth
58880	pAAV-Syn-ChR2(H134R)-GFP	Syn	Activator	Optogenetics	Boyden
59170	pAAV-Syn-Chronos-GFP	Syn	Activator	Optogenetics	Boyden
83898	pAAV-mDlx-ChR2-mCherry-Fishell-3	mDIx	Activator	Optogenetics	Fishell
65014	pAAV-hsyn-Jaws-KGC-GFP-ER2	Syn	Inhibitor	Optogenetics	Boyden
84445	pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2]	CAG	Inhibitor, Cre-dependent	Optogenetics	Boyden
99039	pAAV-CamKII-ArchT-GFP (PV2527)	CamKII	Inhibitor	Optogenetics	Boyden
DREADDs
44361	pAAV-hSyn-DIO-hM3D(Gq)-mCherry	Syn	Activator, Cre-dependent	DREADD	Roth
44362	pAAV-hSyn-DIO-hM4D(Gi)-mCherry	Syn	Inhibitor, Cre-dependent	DREADD	Roth
50474	pAAV-hSyn-hM3D(Gq)-mCherry	Syn	Activator	DREADD	Roth
50475	pAAV-hSyn-hM4D(Gi)-mCherry	Syn	Inhibitor	DREADD	Roth

Currently, no available plasmids.

Browse all Retrograde AAV

Depositor Comments

The AAV retro helper plasmid was generated as a part of the Janelia Research Campus viral project team in collaboration with David Schaffer (University of California, Berkeley).

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