PurposeCre-activated AAV expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20297||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5601
Vector typeMammalian Expression, AAV
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)1647
Mutationhumanized ChR2 gene with histidine 134 changed to arginine, to achieve higher currents
/ Fusion Protein
- mCherry (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site AscI (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Please see http://www.optogenetics.org
for additional information.
Sequence discrepancies found during QC have no functional consequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA was a gift from Karl Deisseroth (Addgene plasmid # 20297)
Generated by Addgene from full sequence.
Generated by Addgene from full sequence supplied by depositor.