1
Plasmid 20297: pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA
  • Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation

  • channelrhodopsin-2

  • hChR2

  • Cop4

  • chr2

  • 1647

  • C. reinhardtii

  • mCherry

  • C terminal on insert

  • humanized ChR2 gene with histidine 134 changed to arginine, to achieve higher currents

  • pAAV
    (Search Vector Database)

  • Stratagene

  • Mammalian Expression ; AAV

  • 5601

  • AscI

  • No

  • NheI

  • No

  • n/a List of Sequencing Primers

  • Ampicillin

  • Stbl3

  • 37

  • High Copy

  • View sequences (3)
  • Karl Deisseroth

  • UBMTA
    Clontech Limited Use Label License
    Ancillary Agreement for Plasmids Containing FP Materials

Comments: 

Please see http://www.optogenetics.org

for additional information.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20297" in your Materials and Methods section.