pAAV-double floxed-eNpHR-EYFP-WPRE-pA
(Plasmid #20949)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pAAV
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 5604
  • Vector type
    Mammalian Expression ; AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    halorhodopsin
  • Alt name
    NpHR
  • Alt name
    eNpHR
  • Species
    C. reinhardtii
  • Insert Size (bp)
    1674
  • Tag / Fusion Protein
    • EYFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please see http://www.optogenetics.org

for additional information.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-double floxed-eNpHR-EYFP-WPRE-pA was a gift from Karl Deisseroth (Addgene plasmid # 20949)