pAAV-Ef1a-DIO eNpHR 3.0-EYFP
- Backbone size w/o insert (bp) 5609
Vector typeMammalian Expression
Growth in Bacteria
Growth instructionsPlease use Rec A- competent cells such as Stbl3 cells from Invitrogen for transformation.
Copy numberHigh Copy
Gene/Insert nameeNpHR 3.0
SpeciesH. sapiens (human)
Insert Size (bp)1683
MutationER export at 3' end of gene. Trafficking signal (TS) in between gene and fluorophore.
/ Fusion Protein
- EYFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site AscI (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer ggccagcttggcacttgatg (Common Sequencing Primers)
Terms and Licenses
This plasmid contains the human elongation factor-1a promoter. Golgi export trafficking signal (TS) sequence is KSRITSEGEYIPLDQIDINV. ER export sequence is FCYENEV.
For additional information please visit - http://www.optogenetics.org
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-Ef1a-DIO eNpHR 3.0-EYFP was a gift from Karl Deisseroth (Addgene plasmid # 26966)
For your References section:Molecular and cellular approaches for diversifying and extending optogenetics. Gradinaru V, Zhang F, Ramakrishnan C, Mattis J, Prakash R, Diester I, Goshen I, Thompson KR, Deisseroth K. Cell. 2010 Apr 2. 141(1):154-65. 10.1016/j.cell.2010.02.037 PubMed 20303157
Map generated by Addgene from full sequence supplied by depositor.
Map uploaded by Addgene staff.
Map uploaded by the depositor.