Addgene - pAAV-Ef1a-DIO eNpHR 3.0-EYFP Plasmid Data

Plasmid 26966: pAAV-Ef1a-DIO eNpHR 3.0-EYFP

Gene/insert name:
eNpHR 3.0
Insert size:
1683
Species:
H. sapiens (human)
Fusion protein or tag:
EYFP
Terminal:
C terminal on insert
Mutation:
ER export at 3' end of gene. Trafficking signal (TS) in between gene and fluorophore.
Vector backbone:
pAAV
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5609
Cloning site 5':
AscI
Site destroyed during cloning:
No
Cloning site 3':
NheI
Site destroyed during cloning:
No
5' sequencing primer:
ggccagcttggcacttgatg List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Please use Rec A- competent cells such as Stbl3 cells from Invitrogen for transformation.
High or low copy:
High Copy
Sequence: View sequences (4)
Map:
View map
Map:
View map
Principal Investigator:
Karl Deisseroth
Terms and Licenses:
MTA

Comments:

This plasmid contains the human elongation factor-1a promoter.

For additional information please visit - http://www.optogenetics.org

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Molecular and cellular approaches for diversifying and extending optogenetics. Gradinaru et al (Cell. 2010 Apr 2. 141(1):154-65. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26966" in your Materials and Methods section.