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Plasmid 20298: pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA
  • Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

  • channelrhodopsin-2

  • hChR2(H134R)

  • cop4

  • ChR2

  • 1662

  • C. reinhardtii

  • EYFP

  • C terminal on insert

  • humanized ChR2 gene with histidine 134 changed to arginine, to achieve higher currents

  • pAAV
    (Search Vector Database)

  • Stratagene

  • Mammalian Expression ; AAV

  • 5604

  • AscI

  • No

  • NheI

  • No

  • n/a List of Sequencing Primers

  • Ampicillin

  • Stbl3

  • 37

  • High Copy

  • View sequences (2)
  • Karl Deisseroth

  • UBMTA
    Ancillary Agreement for Plasmids Containing FP Materials

Comments: 

Please see http://www.optogenetics.org

for additional information.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20298" in your Materials and Methods section.