Adenoviruses are a class of medium-sized, non-enveloped, double-stranded DNA viruses known to cause mild to severe respiratory disease in humans. Advantages of adenoviruses in research include their large packaging capacity (>8 kb), high titers, and high levels of transgene expression. Additionally, these viruses are able to target a broad range of dividing and non-dividing cell types with almost 100% efficiency. Unlike lentiviruses or other retroviruses, adenoviruses do not integrate into the host genome. Disadvantages of adenoviruses include transient transgene expression, as well as the substantial immune response induced by the viral capsid.
Adenoviruses can be produced by several methods, the most common of which involves homologous recombination of adenovirus plasmids in either mammalian cells or microorganisms, including bacteria and yeast. Two plasmids, termed a shuttle plasmid and an adenoviral (also called backbone) plasmid, are recombined into a DNA molecule that incorporates sequences from both plasmids. This DNA molecule can then be transfected into mammalian packaging cell lines to generate adenovirus particles.
Read our Adenoviral Guide for more information about:
- Adenoviral components
- Adenoviral plasmid construction
- Frequently asked questions about adenovirus
- Glossary of commonly used adenovirus terms
Bacterial strains for generating adenovirus.
|16398||BJ5183||Strain for recombination between shuttle plasmid and adenoviral plasmid||Vogelstein|
|16399||AdEasier-1||BJ5183 derivative that contains AdEasy-1™ plasmid||Vogelstein|
Common shuttle and adenoviral plasmids. Want an adenoviral plasmid with your gene of interest? Search our database for adenoviral transfer plasmids that have inserts.
|16400||pAdEasy-1||Adenoviral||For recombining shuttle plasmids||Vogelstein|
|16401||pAdEasy-2||Adenoviral||For recombining shuttle plasmids; for use with large inserts||Vogelstein|
|179201||pAd5-B6 ∆E3-GFP||Adenoviral||A block for the AdenoBuilder genome assembly system. Block 6 with deletion in E3 and insertion of GFP expression cassette.||Bunz|
|179202||pAd5-B6/7 ∆E3-GFP||Adenoviral||A block for the AdenoBuilder genome assembly system; consolidates the regions present in pAd5-B6 ∆E3-GFP and pAd5-B7.||Bunz|
|179203||pAd5-B1-deltaE1-GFP||Adenoviral||A block for the AdenoBuilder genome assembly system. Block 1 with deletion in E1 and insertion of GFP expression cassette.||Bunz|
|16402||pShuttle||Shuttle||For production of viruses containing transgene under a chosen promoter||Vogelstein|
|16403||pShuttle-CMV||Shuttle||For production of viruses containing transgene under CMV promoter||Vogelstein|
|16404||pAdTrack||Shuttle||For production of GFP-trackable viruses containing transgene under a chosen promoter||Vogelstein|
|16405||pAdTrack-CMV||Shuttle||For production of GFP-trackable viruses containing transgene under CMV promoter||Vogelstein|
|16407||pAdEasy 2-GFP beta-gal||Shuttle||Test plasmid that contains β-gal and GFP; contains ∼10Kb genomic DNA stuffer||Vogelstein|
|18703||pEZYshuttle||Shuttle||Modified pShuttle-CMV plasmid with Gateway cassette; See article for additional plasmids||Zhang|
|50957||RedTrackCMV||Shuttle||For production of mRFP-trackable viruses containing transgene under CMV||Bamburg|
|50958||ShuttleNSE||Shuttle||For production of viruses containing transgene under Neuron-specific enolase (NSE) promoter||Bamburg|
|50959||ShuttleMCP||Shuttle||For production of viruses containing transgene under mouse cofilin promoter (MCP)||Bamburg|
|62621||pShuttle-CMV-F2A-T2A-Venus||Shuttle||For production of viruses with multicistronic expression + Venus tag; See plasmid 62622 for plasmid without Venus||Wang|
Plasmids containing modular inserts that span the human adenovirus serotype 5 (HAdV5) genome that can be assembled in a one-tube reaction.
|1000000176||AdenoBuilder toolkit||Plasmids contain modular inserts for seamless assembly of multiple mutations, alterations, or transgene insertions into single full-length adenovirus genomes||Bunz|