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pShuttle-CMV
(Plasmid #16403)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16403 Standard format: Plasmid sent in bacteria as agar stab 1 $75
Cloning Grade DNA 16403-DNA.cg 2 µg of cloning grade DNA in Tris buffer 1 $95

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    None
  • GenBank ID
    AF334399

Cloning Information

Resource Information

Depositor Comments

Vector pShuttle-CMV is used for expression of transgenes under a CMV promoter when no GFP tracer is desired.

Max insert size = 6.6kb with pAdEasy1 and 9.3kb with pAdEasy2.

See attached protocol and http://www.coloncancer.org/adeasy.htm
for more information.

NOTE: There are discrepancies between the Addgene quality control sequence and the assembled sequence from the Vogelstein lab. These differences are not known to affect function.

Information for Cloning Grade DNA (Catalog # 16403-DNA.cg) ( Back to top )

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

  • Amount 2 µg
  • Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
  • Pricing $95 USD
  • Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pShuttle-CMV was a gift from Bert Vogelstein (Addgene plasmid # 16403 ; http://n2t.net/addgene:16403 ; RRID:Addgene_16403)
  • For your References section:

    A simplified system for generating recombinant adenoviruses. He TC, Zhou S, da Costa LT, Yu J, Kinzler KW, Vogelstein B. Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14. 10.1073/pnas.95.5.2509 PubMed 9482916