Purpose(Empty Backbone) Shuttle vector for use in AdEasy System. Used for expression of transgenes under a CMV promoter when no GFP tracer is desired.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16403||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
|Cloning Grade DNA||16403-DNA.cg||2 µg of cloning grade DNA in Tris buffer||1||$95|
This material is available to academics and nonprofits only.
- Backbone size (bp) 7452
Vector typeMammalian Expression, Adenoviral
Growth in Bacteria
Copy numberLow Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Vector pShuttle-CMV is used for expression of transgenes under a CMV promoter when no GFP tracer is desired.
Max insert size = 6.6kb with pAdEasy1 and 9.3kb with pAdEasy2.
See attached protocol and http://www.coloncancer.org/adeasy.htm
for more information.
NOTE: There are discrepancies between the Addgene quality control sequence and the assembled sequence from the Vogelstein lab. These differences are not known to affect function.
Information for Cloning Grade DNA (Catalog # 16403-DNA.cg) ( Back to top )
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $95 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Terms and Licenses
- Not Available to Industry
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pShuttle-CMV was a gift from Bert Vogelstein (Addgene plasmid # 16403 ; http://n2t.net/addgene:16403 ; RRID:Addgene_16403)
For your References section:A simplified system for generating recombinant adenoviruses. He TC, Zhou S, da Costa LT, Yu J, Kinzler KW, Vogelstein B. Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14. 10.1073/pnas.95.5.2509 PubMed 9482916