Addgene - pAdTrack-CMV Plasmid Data

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16405: pAdTrack-CMV
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Plasmid 16405: pAdTrack-CMV

Gene/insert name:
None
Vector backbone:
pAdTrack-CMV
(Search Vector Database)
Vector type:
Mammalian Expression, Adenoviral
Backbone size w/o insert:
9220
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH10B
Growth temperature (℃):
37
High or low copy:
Low Copy
Sequence: View sequences (5)
Map:
View map
Supplemental document:
AdEasy Protocol (application/pdf)
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Bert Vogelstein
Terms and Licenses:
MTA

Comments:

Vector pAdTrack-CMV is used for expression of transgenes under a CMV promoter when a GFP tracer is desired.

Max insert size = 5.0kb with pAdEasy1 and 7.7kb with pAdEasy2.

See attached protocol and http://www.coloncancer.org/adeasy.htm
for more information.

Note that there are discrepancies between Addgene's quality control sequence and the assembled sequenced from the depositing lab. These differences are not known to affect the function of the plasmid.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16405" in your Materials and Methods section.