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A simplified system for generating recombinant adenoviruses.
He TC, Zhou S, da Costa LT, Yu J, Kinzler KW, Vogelstein B
Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
16398BJ5183 cells
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16399AdEasier-1 cells (strain)
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16400pAdEasy-1Adenoviral vector for use in AdEasy System.
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16401pAdEasy-2Adenoviral vector for use in AdEasy System, for use with large inserts.
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16402pShuttleShuttle vector for us in AdEasy System. Used for expression of transgenes under a chosen (non-CMV) promoter when no GFP tracer is desired.
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16403pShuttle-CMVShuttle vector for use in AdEasy System. Used for expression of transgenes under a CMV promoter when no GFP tracer is desired.
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16404pAdTrackShuttle vector for us in AdEasy System. Used for expression of transgenes under a chosen (non-CMV) promoter when a GFP tracer is desired.
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16405pAdTrack-CMVShuttle vector for us in AdEasy System. For expression of transgenes under a CMV promoter when a GFP tracer is desired.
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16407pAdEasy 2-GFP beta-gal
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