Addgene - pAdEasy 2-GFP beta-gal Plasmid Data

Plasmid 16407: pAdEasy 2-GFP beta-gal

Gene/insert name:
beta-galactosidase with stuffer
Alt name:
LacZ
Insert size:
10000
Species:
E. coli
Vector backbone:
pAdEasy-2
(Search Vector Database)
Vector type:
Mammalian Expression, Adenoviral
Backbone size (bp):
30000
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Sequence: View sequences (2)
Supplemental document:
AdEasy Protocol (application/pdf)
Principal Investigator:
Bert Vogelstein
Terms and Licenses:
MTA

Comments:

beta-gal with stuffer (total 10kb) was cloned into pAdTrack-CMV, then recombined into pAdEasy-2.

AdEasy is a registered trademark of the Johns Hopkins University.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A simplified system for generating recombinant adenoviruses. He et al (Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16407" in your Materials and Methods section.