pAdEasy 2-GFP beta-gal
(Plasmid #16407)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pAdEasy-2
  • Backbone size (bp) 30000
  • Vector type
    Mammalian Expression, Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    beta-galactosidase with stuffer
  • Alt name
    LacZ
  • Species
    E. coli
  • Insert Size (bp)
    10000

Cloning Information

Resource Information

Depositor Comments

beta-gal with stuffer (total 10kb) was cloned into pAdTrack-CMV, then recombined into pAdEasy-2.

AdEasy is a registered trademark of the Johns Hopkins University.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAdEasy 2-GFP beta-gal was a gift from Bert Vogelstein (Addgene plasmid # 16407)
  • For your References section:

    A simplified system for generating recombinant adenoviruses. He TC, Zhou S, da Costa LT, Yu J, Kinzler KW, Vogelstein B. Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14. 10.1073/pnas.95.5.2509 PubMed 9482916