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Bacterial strain 16399: AdEasier-1 cells (strain)
  • AdEasier-1 bacterial cells

  • BJAdEasy Cells

  • AdEasier Cells

  • E. coli

  • AY370909

  • n/a
    (Search Vector Database)

  • n/a List of Sequencing Primers

  • Ampicillin and Streptomycin

  • DH5alpha

  • 37

  • Unknown

  • View sequences (1)
  • AdEasy Protocol (application/pdf)

  • Bert Vogelstein

  • UBMTA

Comments: 

AdEasier-1 cells are BJ5183 derivatives which already contain the AdEasy-1 plasmid. Please note that you cannot isolate AdEasy-1 from this strain. Please see Plasmid #16400 if you only want the plasmid. In order to generate recombinant vectors ready to transfer into 293 cells, one simply has to transform the AdEasier-1 cells with a shuttle vector (such as pAdTrack-CMV) containing the gene of interest, and then proceed as with the standard AdEasy system.

See attached protocol and http://www.coloncancer.org/adeasy.htm and http://www.coloncancer.org/adeasy/adeasy_got_ea... for more information.

AdEasier is a trademark of the Johns Hopkins University.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A simplified system for generating recombinant adenoviruses. He et al (Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16399" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only