Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is open for ordering and depositing; find up-to-date details here. To learn more about how we are supporting COVID-19 research and to find related plasmids, check out our COVID-19 and Coronavirus Plasmids & Resources page.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

AdEasier-1 cells (strain)
(Bacterial strain #16399)


Item Catalog # Description Quantity Price (USD)
Bacterial Strain 16399 Bacteria in agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Streptomycin
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    AdEasier-1 bacterial cells
  • Alt name
    BJAdEasy Cells
  • Alt name
    AdEasier Cells
  • Species
    E. coli
  • GenBank ID

Cloning Information

Resource Information

Depositor Comments

AdEasier-1 cells are BJ5183 derivatives which already contain the AdEasy-1 plasmid. Please note that you cannot isolate AdEasy-1 from this strain. Please see Plasmid #16400 if you only want the plasmid. In order to generate recombinant vectors ready to transfer into 293 cells, one simply has to transform the AdEasier-1 cells with a shuttle vector (such as pAdTrack-CMV) containing the gene of interest, and then proceed as with the standard AdEasy system.

AdEasier is a trademark of the Johns Hopkins University.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AdEasier-1 cells (strain) was a gift from Bert Vogelstein (Addgene plasmid # 16399)
  • For your References section:

    A simplified system for generating recombinant adenoviruses. He TC, Zhou S, da Costa LT, Yu J, Kinzler KW, Vogelstein B. Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14. 10.1073/pnas.95.5.2509 PubMed 9482916