AdEasier-1 cells (strain)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Bacterial Strain||16399||Bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Growth in Bacteria
Bacterial Resistance(s)Ampicillin and Streptomycin
Gene/Insert nameAdEasier-1 bacterial cells
Alt nameBJAdEasy Cells
Alt nameAdEasier Cells
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
AdEasier-1 cells are BJ5183 derivatives which already contain the AdEasy-1 plasmid. Please note that you cannot isolate AdEasy-1 from this strain. Please see Plasmid #16400 if you only want the plasmid. In order to generate recombinant vectors ready to transfer into 293 cells, one simply has to transform the AdEasier-1 cells with a shuttle vector (such as pAdTrack-CMV) containing the gene of interest, and then proceed as with the standard AdEasy system.
See attached protocol and http://www.coloncancer.org/adeasy.htm and http://www.coloncancer.org/adeasy/adeasy_got_easier.htm for more information.
AdEasier is a trademark of the Johns Hopkins University.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:AdEasier-1 cells (strain) was a gift from Bert Vogelstein (Addgene plasmid # 16399)
For your References section:A simplified system for generating recombinant adenoviruses. He TC, Zhou S, da Costa LT, Yu J, Kinzler KW, Vogelstein B. Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14. 10.1073/pnas.95.5.2509 PubMed 9482916