Addgene - AdEasier-1 cells (strain) Plasmid Data

Bacterial strain 16399: AdEasier-1 cells (strain)

Gene/insert name:
AdEasier-1 bacterial cells
Alt name:
BJAdEasy Cells
Alt name:
AdEasier Cells
Species:
E. coli
GenBank ID:
AY370909
Vector backbone:
n/a
(Search Vector Database)
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin, Streptomycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Supplemental document:
AdEasy Protocol (application/pdf)
Principal Investigator:
Bert Vogelstein
Terms and Licenses:
MTA

Comments:

AdEasier-1 cells are BJ5183 derivatives which already contain the AdEasy-1 plasmid. Please note that you cannot isolate AdEasy-1 from this strain. Please see Plasmid #16400 if you only want the plasmid. In order to generate recombinant vectors ready to transfer into 293 cells, one simply has to transform the AdEasier-1 cells with a shuttle vector (such as pAdTrack-CMV) containing the gene of interest, and then proceed as with the standard AdEasy system.

See attached protocol and http://www.coloncancer.org/adeasy.htm and http://www.coloncancer.org/adeasy/adeasy_got_ea... for more information.

AdEasier is a trademark of the Johns Hopkins University.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A simplified system for generating recombinant adenoviruses. He et al (Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16399" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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