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AdEasier-1 cells (strain)
(Bacterial strain #16399)

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Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Streptomycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    AdEasier-1 bacterial cells
  • Alt name
    BJAdEasy Cells
  • Alt name
    AdEasier Cells
  • Species
    E. coli
  • GenBank ID
    AY370909

Cloning Information

Resource Information

Depositor Comments

AdEasier-1 cells are BJ5183 derivatives which already contain the AdEasy-1 plasmid. Please note that you cannot isolate AdEasy-1 from this strain. Please see Plasmid #16400 if you only want the plasmid. In order to generate recombinant vectors ready to transfer into 293 cells, one simply has to transform the AdEasier-1 cells with a shuttle vector (such as pAdTrack-CMV) containing the gene of interest, and then proceed as with the standard AdEasy system.

See attached protocol and http://www.coloncancer.org/adeasy.htm and http://www.coloncancer.org/adeasy/adeasy_got_easier.htm for more information.

AdEasier is a trademark of the Johns Hopkins University.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AdEasier-1 cells (strain) was a gift from Bert Vogelstein (Addgene plasmid # 16399)
  • For your References section:

    A simplified system for generating recombinant adenoviruses. He TC, Zhou S, da Costa LT, Yu J, Kinzler KW, Vogelstein B. Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14. 10.1073/pnas.95.5.2509 PubMed 9482916