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(Plasmid #16402)

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Available to Academic and Nonprofits Only


  • Vector backbone
  • Backbone size (bp) 6736
  • Vector type
    Mammalian Expression, Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy

Sequence Information


  • Gene/Insert name
  • GenBank ID

Cloning Information

Resource Information

Depositor Comments

Vector pShuttle is used for expression of transgenes under a chosen (non-CMV) promoter when no GFP tracer is desired.

Max insert size = 7.5kb with pAdEasy1 and 10.2kb with pAdEasy2.

See attached protocol and

for more information.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pShuttle was a gift from Bert Vogelstein (Addgene plasmid # 16402)
  • For your References section:

    A simplified system for generating recombinant adenoviruses. He TC, Zhou S, da Costa LT, Yu J, Kinzler KW, Vogelstein B. Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14. 10.1073/pnas.95.5.2509 PubMed 9482916