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Plasmid 16402: pShuttle
  • Shuttle vector for us in AdEasy System. Used for expression of transgenes under a chosen (non-CMV) promoter when no GFP tracer is desired.

  • None

  • AF334399

  • pShuttle
    (Search Vector Database)

  • Mammalian Expression, Adenoviral

  • 6736

  • n/a List of Sequencing Primers

  • Kanamycin

  • DH10B

  • 37

  • Low Copy

  • View sequences (2)
  • View map

  • AdEasy Protocol (application/pdf)

  • Bert Vogelstein

  • UBMTA

Comments: 

Vector pShuttle is used for expression of transgenes under a chosen (non-CMV) promoter when no GFP tracer is desired.

Max insert size = 7.5kb with pAdEasy1 and 10.2kb with pAdEasy2.

See attached protocol and http://www.coloncancer.org/adeasy.htm

for more information.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A simplified system for generating recombinant adenoviruses. He et al (Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16402" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only