Addgene - pShuttle Plasmid Data

Plasmid 16402: pShuttle

Gene/insert name:
None
GenBank ID:
AF334399
Vector backbone:
pShuttle
(Search Vector Database)
Vector type:
Mammalian Expression, Adenoviral
Backbone size (bp):
6736
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH10B
Growth temperature (℃):
37
High or low copy:
Low Copy
Sequence: View sequences (2)
Map:
View map
Supplemental document:
AdEasy Protocol (application/pdf)
Principal Investigator:
Bert Vogelstein
Terms and Licenses:
MTA

Comments:

Vector pShuttle is used for expression of transgenes under a chosen (non-CMV) promoter when no GFP tracer is desired.

Max insert size = 7.5kb with pAdEasy1 and 10.2kb with pAdEasy2.

See attached protocol and http://www.coloncancer.org/adeasy.htm

for more information.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A simplified system for generating recombinant adenoviruses. He et al (Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16402" in your Materials and Methods section.