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pAdEasy-1
(Plasmid #16400)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16400 Standard format: Plasmid sent in bacteria as agar stab 1 $75
Cloning Grade DNA 16400-DNA.cg 2 µg of cloning grade DNA in Tris buffer 1 $95

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAdEasy and reg-1
  • Backbone size (bp) 33414
  • Vector type
    Mammalian Expression, Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    None
  • GenBank ID
    AY370909

Cloning Information

Resource Information

Depositor Comments

Recombine plasmids from the shuttle vectors (pShuttle, pShuttle-CMV, pAdTrack, pAdTrack-CMV) into the adenoviral plasmid pAdEasy-1.

Plasmids recombined in pAdEasy-1 should be packaged in either 293 or 911 cells to make virus.

See attached protocol and http://www.coloncancer.org/adeasy.htm

for more information.

AdEasy is a registered trademark of the Johns Hopkins University.

NOTE: Addgene was unable to obtain high quality sequence for this plasmid, which may due to its size and repeat regions. Please take this into consideration when conducting your own quality control.

Information for Cloning Grade DNA (Catalog # 16400-DNA.cg) ( Back to top )

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

  • Amount 2 µg
  • Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
  • Pricing $95 USD
  • Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAdEasy-1 was a gift from Bert Vogelstein (Addgene plasmid # 16400 ; http://n2t.net/addgene:16400 ; RRID:Addgene_16400)
  • For your References section:

    A simplified system for generating recombinant adenoviruses. He TC, Zhou S, da Costa LT, Yu J, Kinzler KW, Vogelstein B. Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14. 10.1073/pnas.95.5.2509 PubMed 9482916