Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Bacterial Strain||16398||Bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Growth in Bacteria
Gene/Insert nameBJ5183 bacterial cells
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
This is a bacterial strain - not a plasmid. Select with 30 ug/mL streptomycin.
Electrocompetent BJ5183 cells are used to carry out the recombination between the shuttle vector (pShuttle, pShuttle-CMV, pAdTrack, or pAdTrack-CMV) and the adenoviral vector (pAdEasy1 or pAdEasy2).
See attached protocol and http://www.coloncancer.org/adeasy.htm
for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:BJ5183 cells was a gift from Bert Vogelstein (Addgene plasmid # 16398)
For your References section:A simplified system for generating recombinant adenoviruses. He TC, Zhou S, da Costa LT, Yu J, Kinzler KW, Vogelstein B. Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14. 10.1073/pnas.95.5.2509 PubMed 9482916