Addgene - BJ5183 cells Plasmid Data

Bacterial strain 16398: BJ5183 cells

Gene/insert name:
BJ5183 bacterial cells
Species:
E. coli
Vector backbone:
n/a
(Search Vector Database)
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Streptomycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Supplemental document:
AdEasy Protocol (application/pdf)
Principal Investigator:
Bert Vogelstein
Terms and Licenses:
MTA

Comments:

This is a bacterial strain - not a plasmid. Select with 30 ug/mL streptomycin.

Electrocompetent BJ5183 cells are used to carry out the recombination between the shuttle vector (pShuttle, pShuttle-CMV, pAdTrack, or pAdTrack-CMV) and the adenoviral vector (pAdEasy1 or pAdEasy2).

See attached protocol and http://www.coloncancer.org/adeasy.htm

for more information.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A simplified system for generating recombinant adenoviruses. He et al (Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16398" in your Materials and Methods section.