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viral vector icon Lentiviral Plasmids


Lentiviruses, a subset of retroviruses, are some of the most common and useful viral vectors in the lab. Lentivirus can transduce both dividing and non-dividing cells without a significant immune response. They also integrate into the host genome, permitting long-term transgene expression. There are some safety considerations to evaluate when working with lentivirus: these vectors are based on HIV-1, which may require additional lab biosafety procedures and, additionally, lentiviral integration has the potential for oncogenesis. The 2nd and 3rd generation lentiviral systems available from Addgene have been designed for increased researcher safety.

Read our Lentiviral Guide for more information about:

  • Lentiviral components
  • 2nd vs 3rd generation lentiviral systems
  • Viral production
  • Frequently asked questions about lentivirus use
  • Glossary of commonly used lentiviral terms

Popular Lentiviral Envelope and Packaging Plasmids

Common lentiviral envelope and packaging plasmids from 2nd and 3rd generations.

   ID Plasmid Generation Type Description PI
12253 pRSV-Rev 3rd Packaging Contains Rev; can be used with 3rd generation transfer plasmids along with additional packaging components Trono Add to Cart
12251 pMDLg/pRRE 3rd Packaging Contains Gag and Pol; can be used with 3rd generation transfer plasmids along with a plasmid encoding Rev Trono Add to Cart
12260 psPAX2 2nd Packaging Contains Gag, Pol, Rev, and Tat; can be used with 2nd and 3rd generation transfer vectors Trono Add to Cart
12259 pMD2.G N/A Envelope VSV-G-expressing envelope vector Trono Add to Cart
8454 pCMV-VSV-G N/A Envelope VSV-G-expressing envelope vector Weinberg Add to Cart
8455 pCMV-dR8.2 dvpr 2nd Packaging Contains Gag, Pol, Rev, Tat, Vif, and Nef; can be used with 2nd and 3rd generation transfer vectors Weinberg Add to Cart
1733 pCI-VSVG N/A Envelope VSV-G recommended for the FELIX (FIV-based) system Nolan Add to Cart
22502 pCEP4-tat N/A Packaging Expression of tat; increases vector titer Kasparov Add to Cart
22501 pHEF-VSVG N/A Envelope VSV-G-expressing envelope vector Kasparov Add to Cart
22500 pNHP 2nd Packaging Contains gag, pol, tat, rev Kasparov Add to Cart
1732 pCPRDEnv 2nd Packaging part of the FELIX vector system, expresses Gag-Pol and Rev Nolan Add to Cart
35617 pCAG-Eco N/A Envelope Ecotropic MLV expressing envelope vector Nienhuis and Salmon Add to Cart
35616 pCAG-VSVG N/A Envelope VSV-G-expressing envelope vector Nienhuis and Salmon Add to Cart
17532 pLTR-G 2nd Envelope Expression of VSV-G glycoprotein (Indiana strain). Use with packaging construct encoding Tat. Reiser Add to Cart
17531 pCD/NL-BH*DDD 2nd Packaging Contains Gag/Pol, Tat, Rev. Use with envelope plasmids from Jakob Reiser. Reiser Add to Cart
17576 pLTR-RD114A 2nd Envelope Hybrid of RD114 Env GP and MLV 4070A Env GP. Good for work in human MSCs. Use packaging construct encoding Tat. Reiser Add to Cart

Popular Lentiviral Transfer Plasmids

Common lentiviral transfer plasmids from 2nd and 3rd generations.

Looking for lentiviral vectors to express your CRISPR gRNA? Please see our CRISPR Empty gRNA vectors table and search "lenti".

   ID Plasmid Generation Description PI
8453 pLKO.1 puro 3rd U6-driven shRNA empty vector with puro resistance. See plasmid 24150 for hygro resistance. Weinberg Add to Cart
10878 pLKO.1 – TRC cloning vector 3rd U6-driven shRNA empty vector; includes a stuffer for easy cloning Root Add to Cart
14748 pLKO.3G 3rd U6-driven shRNA empty vector with EGFP marker. See plasmid 14749 for Thy1.1 selection. Benoist and Mathis Add to Cart
21915 Tet-pLKO-puro 3rd inducible expression of shRNA; puromycin selection. See plasmid 21916 for Neo selection. Wiederschain Add to Cart
11578 pSico 3rd Conditional (Cre-lox), stable expression of shRNAs; addition of Cre turns on shRNA expression. See article for more cre-lox shRNA expression variants. Jacks Add to Cart
19319 pLJM1-EGFP 3rd CMV-driven EGFP fusion; can be used for cDNA expression; puro resistance Sabatini Add to Cart
14883 FUGW 3rd hUbC-driven EGFP; can be used for cDNA expression Baltimore Add to Cart
12247 pLVTHM 2nd EF-1a-driven GFP and shRNA under the control of a tet-responsive H1 promoter Trono Add to Cart
11651 pLVUT-tTR-KRAB 2nd Inducible expression of transgene or shRNA. See article for detailed information about cloning & additional plasmids. Aebischer and Trono Add to Cart
11795 pLL3.7 3rd Expresses shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Parijs Add to Cart
11619 pLB 2nd Modification of pLL3.7; Genetic elements known to prevent epigenetic silencing were added; Expresses shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Kissler Add to Cart
1730 pLionII 3rd FIV-derived (FELIX) vector with a chimeric 5’LTR, Any expression cassette (promoter and gene of interest) can be cloned into the vector. Nolan Add to Cart
12257 pWPXL 2nd EF-1alpha driven constitutive transgene expression, contains intron that gives you high expression. Trono Add to Cart
12254 pWPI 2nd EF-1alpha driven constitutive transgene expression and EGFP coexpression. Trono Add to Cart
17619 EF.CMV.RFP 2nd EF-1 alpha promoter for transgene and CMV drives expression of RFP as a reporter. See plasmid 17618 for GFP plasmid. Cheng Add to Cart
17452 pLenti CMV Puro DEST 3rd Gateway destination vectors with constitutive or inducible expression of cDNAs, shRNAs or miRNAs, and use a wide variety of drug selection markers. See article for more variations. Campeau Add to Cart
39481 pLenti-puro 3rd CMV driven expression of cDNA. Puro selection. Shih Add to Cart
25737 pSLIK-Hygro 3rd Tet-based inducible shRNA or cDNA expression, gateway vector, constitutive Hygromycin resistance gene coexpression. See article for other versions of pSLIK. Fraser Add to Cart
15948 pLOVE 3rd Tet inducible gateway destination vector for cDNA expression Ramalho-Santos Add to Cart
24129 pULTRA 3rd bi-cistronic expression of EGFP and the gene of interest, also can express shRNA (cloning H1-shRNA cassettes into the unique SnaBI site in the 3´-LTR). See here for other versions of pULTRA. Moore Add to Cart
19319 pLJM1-EGFP 3rd for EGFP fusion; PGK driven puromycin Sabatini Add to Cart
25895 pLX301 3rd Gateway vector for CMV driven expression of cDNA. See article for alternative versions of this plasmid. Root Add to Cart
44012 pInducer20 3rd Tet-inducible lentiviral vector for ORF expression, multicistronic design enables tracking of viral transduction. Also see plasmid 46948 . Elledge Add to Cart
44011 pInducer10-mir-RUP-PheS 3rd Lentiviral shRNA vector (pGIPz based) for inducible knockdown, multicistronic design enables tracking of viral transduction. See article for more similar plasmids. Elledge Add to Cart
21373 pHIV-EGFP 3rd EF-1alpha driven expression of cDNA and and EGFP co-expression. See plasmid 21374 for dtomato version. Welm Add to Cart
21916 Tet-pLKO-neo 3rd inducible expression of shRNA; neomycin selection. See plasmid 21915 for Puro resistance. Wiederschain Add to Cart
36084 pLV-mCherry 3rd CMV driven expression of mCherry fusion. See plasmids 36083 and 36086 for other FP variants. Tsoulfas Add to Cart
41393 pCW57.1 3rd Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puro. See article for more versions of this plasmid. Root Add to Cart


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