Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22500||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Growth instructionsTransform into DH5alpha and grow in Luria Broth containing 100µg/ml ampicillin.
Copy numberHigh Copy
Gene/Insert namegag, pol, tat, rev
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
This plasmid forms part of a plasmid set for lentivirus generation in the Kasparov lab, University of Bristol, UK.
Addgene was unable to obtain quality control sequence for this plasmid. The depositing lab suggested the following diagnostic digest: HindIII digest, expect 0.55, 1.0, and 3.8 kb fragments. Our results for the digest can be seen under "Reviews" on this page.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pNHP was a gift from Sergey Kasparov (Addgene plasmid # 22500 ; http://n2t.net/addgene:22500 ; RRID:Addgene_22500)
For your References section:Efficient large-scale production and concentration of HIV-1-based lentiviral vectors for use in vivo. Coleman JE, Huentelman MJ, Kasparov S, Metcalfe BL, Paton JF, Katovich MJ, Semple-Rowland SL, Raizada MK. Physiol Genomics. 2003 Feb 6. 12(3):221-8. 10.1152/physiolgenomics.00135.2002 PubMed 12488511