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pHEF-VSVG
(Plasmid #22501)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22501 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Transform into DH5alpha and grow in Luria Broth containing 100µg/ml ampicillin.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Vesicular Stomatitis Virus Glycoprotein
  • Species
    Rhabdoviridae

Cloning Information

Resource Information

  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    Original plasmid sent by Jason Coleman and Matthew Huentelman.
  • Terms and Licenses
  • Article Citing this Plasmid

Depositor Comments

This plasmid forms part of a plasmid set for lentivirus generation in the Kasparov lab, University of Bristol, UK.

Addgene was unable to obtain quality control sequence for this plasmid. The depositing lab suggested the following diagnostic digest: PstI digest, expect 0.55, 1.3, and 4.0 kb fragments. Our results for the digest can be seen under "Addgene Notes" on this page.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHEF-VSVG was a gift from Sergey Kasparov (Addgene plasmid # 22501)
  • For your References section:

    Efficient large-scale production and concentration of HIV-1-based lentiviral vectors for use in vivo. Coleman JE, Huentelman MJ, Kasparov S, Metcalfe BL, Paton JF, Katovich MJ, Semple-Rowland SL, Raizada MK. Physiol Genomics. 2003 Feb 6. 12(3):221-8. 10.1152/physiolgenomics.00135.2002 PubMed 12488511