Plasmid 8454: pCMV-VSV-G
  • Envelope protein for producing lentiviral and MuLV retroviral particles. Use in conjunction with a packaging vector such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral).

  • None

  • na
    (Search Vector Database)

  • Mammalian Expression

  • 6363

  • T7 List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (4)
  • Weinberg-viral (application/pdf)

  • Notes from Addgene (2)

  • Bob Weinberg



VSVG envelope protein, for use with lentiviral and MuLV vectors. The 293T cell line can be obtained from the Weinberg lab or GenHunter http://genhunter.com/products/aptag-3/index.html

Please note that depositor's sequence is not 100% complete. The flanking sequence of VSV-G actually contains XhoI (at both the 5' and 3'end). You can see sequence for this area in the Reviews link to the right..

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Lentivirus-delivered stable gene silencing by RNAi in primary cells. Stewart et al (RNA 2003 Apr;9(4):493-501. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8454" in your Materials and Methods section.