Addgene - pCMV-VSV-G Plasmid Data

Plasmid 8454: pCMV-VSV-G

Gene/insert name:
None
Vector backbone:
na
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size (bp):
6363
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (4)
Supplemental document:
Weinberg-viral (application/pdf)
Supplemental document:
Notes from Addgene (2)
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

VSVG envelope protein, for use with lentiviral and MuLV vectors. The 293T cell line can be obtained from the Weinberg lab or GenHunter http://genhunter.com/products/aptag-3/index.html

Please note that depositor's sequence is not 100% complete. The flanking sequence of VSV-G actually contains XhoI (at both the 5' and 3'end). You can see sequence for this area in the Reviews link to the right..

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Lentivirus-delivered stable gene silencing by RNAi in primary cells. Stewart et al (RNA 2003 Apr;9(4):493-501. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8454" in your Materials and Methods section.