Addgene - pUMVC Plasmid Data

Plasmid 8449: pUMVC

Gene/insert name:
None
Vector backbone:
pUMVC
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
5' sequencing primer:
CMV Forward List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Sequence: View sequences (2)
Supplemental document:
Weinberg-viral (application/pdf)
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

Packaging plasmid for MuLV constructs, MuLV gag and pol (no envelope). The 293T cell line can be obtained from the Weinberg lab or GenHunter http://genhunter.com/products/aptag-3/index.html

See pUMVC3-gag-pol for a map/sequence.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Lentivirus-delivered stable gene silencing by RNAi in primary cells. Stewart et al (RNA 2003 Apr;9(4):493-501. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8449" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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