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psPAX2
(Plasmid #12260)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12260 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    psPAX2
  • Backbone size (bp) 10703
  • Vector type
    Mammalian Expression, Lentiviral ; Packaging

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

2nd generation packaging vector. Known to work with many of the Aebischer and Trono lab lentiviral vectors, as well as the pLKO system. Produces higher titer than pCMV-dR8.2 dvpr.

Note: This plasmid may run as a dimer. Try testing multiple colonies to select the monomer. It may be helpful to follow protocols for low copy plasmids.

Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    psPAX2 was a gift from Didier Trono (Addgene plasmid # 12260 ; http://n2t.net/addgene:12260 ; RRID:Addgene_12260)