Addgene - psPAX2 Plasmid Data

Plasmid 12260: psPAX2

Gene/insert name:
None
Vector backbone:
psPAX2
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral ; Packaging
Backbone size (bp):
10703
5' sequencing primer:
See map List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Map:
View map
Supplemental document:
BamHI and EcoRI digests (application/pdf)
Principal Investigator:
Didier Trono
Terms and Licenses:
MTA

Comments:

2nd generation packaging vector. Known to work with many of the Aebischer and Trono lab lentiviral vectors, as well as the pLKO system. Produces higher titer than pCMV-dR8.2 dvpr.

Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12260" in your Materials and Methods section.