1
Plasmid 10878: pLKO.1 - TRC cloning vector
  • stuffer

  • 1900

  • pLKO.1
    (Search Vector Database)

  • Mammalian Expression, Lentiviral, RNAi

  • 7032

  • AgeI

  • No

  • EcoRI

  • No

  • LKO.1 5' List of Sequencing Primers

  • Ampicillin

  • Stbl3

  • 37

  • Low Copy

  • Puromycin

  • View sequences (2)
  • View map

  • Addgene's pLKO.1 protocol

  • Notes from Addgene (1)

  • David Root

  • UBMTA

Comments: 

This is the recommended vector for cloning and expressing new shRNA sequences. This plasmid is being used by The RNAi Consortium to produce their shRNA library http://www.broad.mit.edu/genome_bio/trc/ The 1.9kb stuffer can be released with AgeI and EcoRI and replaced with your shRNA sequence of choice.

The link to the author's map shows the key features of this vector before the 1.9kb stuffer was cloned in. The link to the sequence shows the entire sequence with the stuffer sequence in capital letters.

Also, see Addgene's pLKO.1 protocol http://www.addgene.org/plko on how to use the pLKO.1 vector.

Plasmid grows more slowly than standard plasmids.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen. Moffat et al (Cell. 2006 Mar 24. 124(6):1283-98. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 10878" in your Materials and Methods section.