1
Plasmid 10879: pLKO.1 - TRC control
  • non-hairpin 18bp

  • 18

  • pLKO.1
    (Search Vector Database)

  • Mammalian Expression, Lentiviral, RNAi

  • 7032

  • AgeI

  • No

  • EcoRI

  • No

  • LKO.1 5' List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • Puromycin

  • View sequences (3)
  • View map

  • Addgene's pLKO.1 protocol

  • Notes from Addgene (1)

  • David Root

  • UBMTA

Comments: 

This plasmid is recommended by The RNAi Consortium for use as a non-hairpin control http://www.broad.mit.edu/genome_bio/trc/ The 18bp between AgeI and EcoRI of this plasmid are CCGCAGGTATGCACGCGT. The link to the author's map shows the key features of this vector before the 18bp were cloned in.

Also, see Addgene's pLKO.1 protocol http://www.addgene.org/plko on how to use the pLKO.1 vector.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen. Moffat et al (Cell. 2006 Mar 24. 124(6):1283-98. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 10879" in your Materials and Methods section.