Several laboratories have deposited plasmids for mammalian RNA interference experiments at Addgene’s non-profit plasmid repository. This website describes some of the empty vectors that you can use to clone shRNAs.
Click here to browse all mammalian RNAi plasmids from Addgene’s repository.
Nature Methods has published a user’s guide to RNA interference in mammalian cells. Further information for many of Addgene’s RNAi vectors can be found in these articles.
To obtain a plasmid, Login or Register a new account. Find your plasmid of interest, then click the Add to Cart button. When you are finished adding plasmids to your cart, complete the checkout process online. Click here for Addgene’s pricing and distribution policy.