|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||10676||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size (bp) 6700
Vector typeMammalian Expression, Retroviral, RNAi
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer pLXSN 5' (Common Sequencing Primers)
Replaced the puromycin resistance gene from pMKO.1-puro with GFP for cell sorting. HindIII-ClaI GFP ORF from phrGFP-c (Stratagene) was cloned into pMKO.1 puro.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMKO.1 GFP was a gift from William Hahn (Addgene plasmid # 10676 ; http://n2t.net/addgene:10676 ; RRID_Addgene_10676)