1
Plasmid 8452: pMKO.1 puro
  • None

  • pMSCV
    (Search Vector Database)

  • Mammalian Expression, Retroviral, RNAi

  • 6700

  • AgeI

  • No

  • EcoRI

  • No

  • pLXSN 5' List of Sequencing Primers

  • pBABE-3

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • Puromycin

  • View sequences (1)
  • View map

  • View map

  • Weinberg-viral (application/pdf)

  • Bob Weinberg

  • UBMTA

Comments: 

Empty MuLV vector for siRNA expression, replaces Retrohair. The 293T cell line can be obtained from the Weinberg lab or GenHunter http://genhunter.com/products/aptag-3/index.html

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Lentivirus-delivered stable gene silencing by RNAi in primary cells. Stewart et al (RNA 2003 Apr;9(4):493-501. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8452" in your Materials and Methods section.