Addgene - pMKO.1 puro p53 shRNA 1 Plasmid Data

Plasmid 10671: pMKO.1 puro p53 shRNA 1

Gene/insert name:
p53 shRNA
Alt name:
p53
Insert size:
58
Species:
H. sapiens (human)
Entrez Gene:
TP53 (BCC7, LFS1, P53, TRP53)
Vector backbone:
pMKO.1
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral, RNAi
Backbone size w/o insert (bp):
6700
Cloning site 5':
AgeI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
pLXSN 5' List of Sequencing Primers
3' sequencing primer:
pBABE-3
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Map:
View map
Map:
View map
Principal Investigator:
William Hahn
Terms and Licenses:
MTA

Comments:

Also reference: Stewart, S.A., Dykxhoorn, D.M., Palliser, D., Mizuno, H., Yu, E.Y., Eisen, H.N., Sabatini, D.M., Hahn, W.C., Sharp, P.A., Weinberg, R.A., et al. (2003). RNA 9, 493�501.

Note: There is no XhoI site in the shRNA oligo, as indicated on the author's map.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Telomerase maintains telomere structure in normal human cells. Masutomi et al (Cell. 2003 Jul 25. 114(2):241-53. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 10671" in your Materials and Methods section.