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pMKO.1 puro Rb shRNA
(Plasmid #10670)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 10670 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMKO.1
  • Backbone size w/o insert (bp) 6700
  • Vector type
    Mammalian Expression, Retroviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rb shRNA
  • Alt name
    Rb
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    58
  • Entrez Gene
    RB1 (a.k.a. OSRC, PPP1R130, RB, p105-Rb, p110-RB1, pRb, pp110)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pLXSN 5'
  • 3′ sequencing primer pBABE-3
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Sequences specific for 3'UTR of Rb transcript. forward, 5'-CCGGCAGAGATCGTGTATTGAGATTCTCGAGAATCTCAATACACGATCTCTGTTTTTG-3'; and reverse, 5'-AATTCAAAAACAGAGATCGTGTATTGAGATTCTCGAGAATCTCAATACACGATCTCTG-3'

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMKO.1 puro Rb shRNA was a gift from William Hahn (Addgene plasmid # 10670 ; http://n2t.net/addgene:10670 ; RRID:Addgene_10670)
  • For your References section:

    Transformation of human and murine fibroblasts without viral oncoproteins. Boehm JS, Hession MT, Bulmer SE, Hahn WC. Mol Cell Biol. 2005 Aug . 25(15):6464-74. 10.1128/MCB.25.15.6464-6474.2005 PubMed 16024784