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pMKO.1 puro GFP shRNA
(Plasmid #10675)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 10675 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMKO.1
  • Backbone size w/o insert (bp) 6700
  • Vector type
    Mammalian Expression, Retroviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP shRNA
  • Alt name
    GFP
  • Species
    jellyfish
  • Insert Size (bp)
    58

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pLXSN 5'
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

shRNA oligo sequence: 5'-GCTACGTCCAGGAGCGCACCCTCGAGGGTGCGCTCCTGGACGTAGCC

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMKO.1 puro GFP shRNA was a gift from William Hahn (Addgene plasmid # 10675)
  • For your References section:

    The telomerase reverse transcriptase regulates chromatin state and DNA damage responses. Masutomi K, Possemato R, Wong JM, Currier JL, Tothova Z, Manola JB, Ganesan S, Lansdorp PM, Collins K, Hahn WC. Proc Natl Acad Sci U S A. 2005 Jun 7. 102(23):8222-7. 10.1073/pnas.0503095102 PubMed 15928077