Addgene - pMKO.1 puro GFP shRNA Plasmid Data

Plasmid 10675: pMKO.1 puro GFP shRNA

Gene/insert name:
GFP shRNA
Alt name:
GFP
Insert size:
58
Species:
jellyfish
Vector backbone:
pMKO.1
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral, RNAi
Backbone size w/o insert (bp):
6700
Cloning site 5':
AgeI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
pLXSN 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (2)
Map:
View map
Map:
View map
Principal Investigator:
William Hahn
Terms and Licenses:
MTA

Comments:

shRNA oligo sequence: 5'-GCTACGTCCAGGAGCGCACCCTCGAGGGTGCGCTCCTGGACGTAGCC

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The telomerase reverse transcriptase regulates chromatin state and DNA damage responses. Masutomi et al (Proc Natl Acad Sci U S A. 2005 Jun 7. 102(23):8222-7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 10675" in your Materials and Methods section.