|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12371||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4500
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)8100
- Cloning method Restriction Enzyme
- 5′ sequencing primer LXSN primer (Common Sequencing Primers)
Packaging plasmid for pCL retroviruses. See author's map for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCL-Eco was a gift from Inder Verma (Addgene plasmid # 12371 ; http://n2t.net/addgene:12371 ; RRID:Addgene_12371)
For your References section:The pCL vector system: rapid production of helper-free, high-titer, recombinant retroviruses. Naviaux RK, Costanzi E, Haas M, Verma IM. J Virol. 1996 Aug . 70(8):5701-5. PubMed 8764092