Addgene - pMD2.G Plasmid Data

Plasmid 12259: pMD2.G

Purpose:
VSV-G envelope expressing plasmid
Gene/insert name:
VSV G
Vector backbone:
pMD2.G
(Search Vector Database)
Vector type:
Mammalian Expression ; Envelope
Backbone size w/o insert (bp):
5824
5' sequencing primer:
CMV Fwd List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Map:
View map
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Didier Trono
Terms and Licenses:
MTA

Comments:

Envelope plasmid. Known to work with most Aebischer and Trono lab lentiviral vectors, as well as the pLKO system.

Please note that the full sequence for this plasmid is approximated and not fully verified (e.g. there is no ClaI site in this plasmid). Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12259" in your Materials and Methods section.