|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12259||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5824
Vector typeMammalian Expression, Lentiviral ; Envelope
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert nameVSV G
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV Fwd (Common Sequencing Primers)
Envelope plasmid. Known to work with most Aebischer and Trono lab lentiviral vectors, as well as the pLKO system.
Please note that the full sequence for this plasmid is approximated and not fully verified (e.g. there is no ClaI site in this plasmid). Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMD2.G was a gift from Didier Trono (Addgene plasmid # 12259)