pMD2.G
(Plasmid #12259)

Purpose

VSV-G envelope expressing plasmid
Depositing Lab

Didier Trono
Publication

Trono Lab Packaging and Envelope Plasmids (unpublished)
Price

$65 (USD)

Add to Cart

Available to Academic and Nonprofits Only

Backbone

Vector backbone
pMD2.G
(Search Vector Database)
Backbone size w/o insert (bp) 5824
Vector type
Mammalian Expression ; Envelope

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin
Growth Temperature
37°C
Growth Strain(s)
DH5alpha
Copy number
High Copy

Sequence Information

Depositor Sequences

Full (1)
Addgene Sequences

Partial (3)

Gene/Insert

Gene/Insert name
VSV G

Cloning Information

Cloning method Restriction Enzyme
5′ sequencing primer CMV Fwd

(Common Sequencing Primers)

Resource Information

Addgene Notes
- Addgene diagnostic digest
Terms and Licenses
- UBMTA

Depositor Comments

Envelope plasmid. Known to work with most Aebischer and Trono lab lentiviral vectors, as well as the pLKO system.

Please note that the full sequence for this plasmid is approximated and not fully verified (e.g. there is no ClaI site in this plasmid). Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

pMD2.G was a gift from Didier Trono (Addgene plasmid # 12259)

Map generated by Addgene from full sequence supplied by depositor.

Map uploaded by the depositor.

	More than 20 requests
	More than 50 requests
	More than 100 requests

pMD2.G (Plasmid #12259)