pMD2.G
(Plasmid #12259)

• Purpose: VSV-G envelope expressing plasmid
• Depositing Lab: Didier Trono
• Publication: Trono Lab Packaging and Envelope Plasmids (unpublished)

Backbone

• Vector backbone: pMD2.G
  (Search Vector Database)
• Backbone size w/o insert (bp): 5824
• Vector type: Mammalian Expression, Lentiviral ; Envelope

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VSV G

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV Fwd

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest- NotI, HindIII
  • Addgene Diagnostic Digest- BamHI
  • Addgene Diagnostic Digest- PvuI-HF and ApaI
  • Addgene Diagnostic Digest- EcoRI
• Terms and Licenses:
  • UBMTA
• Industry Terms:
  • Not Available to Industry
• Articles Citing this Plasmid:
  • 2960 References

Depositor Comments

Please note that immediate availability of this material is restricted to U.S. customers only. For international customers, an export license is required. If you are interested in obtaining this plasmid, please contact

Envelope plasmid. Known to work with most Aebischer and Trono lab lentiviral vectors, as well as the pLKO system.

Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more.