Addgene - scramble shRNA Plasmid Data

Plasmid 1864: scramble shRNA

Gene/insert name:
scramble
Insert size:
60
Vector backbone:
pLKO.1
(Search Vector Database)
Backbone manufacturer:
Stewart SA, RNA 2003 Apr; 9(4):493-501.
Vector type:
Lentiviral, RNAi
Backbone size w/o insert (bp):
7032
Cloning site 5':
AgeI
Site destroyed during cloning:
Yes
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
LKO.1 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Unknown
Growth temperature (℃):
37
Growth instructions:
XL10-Gold Ultracompetent Cells from Stratagene. 37oC.
High or low copy:
Unknown
Selectable markers:
Puromycin
Sequence: View sequences (3)
Map:
View map
Supplemental document:
Addgene's pLKO.1 protocol
Principal Investigator:
David Sabatini
Terms and Licenses:
MTA

Comments:

shRNA for use as a negative control. Sequence of hairpin is:CCTAAGGTTAAGTCGCCCTCGCTCGAGCGAGGGCGACTTAACCTTAGG. For packaging, please use pCMV-dR8.2 dvpr (Addgene plasmid #8455) and pCMV-VSVG (Addgene plasmid #8454).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Sarbassov et al (Science 2005 Feb 18;307(5712):1098-101. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1864" in your Materials and Methods section.