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Plasmid 8453: pLKO.1 puro
  • 3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses puromycin for selection.

  • None

  • LKO.1

  • pLKO.1
    (Search Vector Database)

  • Mammalian Expression, Lentiviral, RNAi

  • 7032

  • AgeI

  • No

  • EcoRI

  • No

  • LKO.1 5' List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • Puromycin

  • View sequences (2)
  • Addgene's pLKO.1 protocol

  • Notes from Addgene (1)

  • Bob Weinberg

  • UBMTA

Comments: 

Empty lentiviral vector for siRNA expression; replaces Lentihair; see author's map. The 293T cell line can be obtained from the Weinberg lab or GenHunter http://genhunter.com/products/aptag-3/index.html

Note that Addgene's quality control sequence shows that there are about 25 nucleotides between the AgeI and EcoRI sites (the depositor sequences is not as accurate in this region).

For packaging, please use pCMV-dR8.2 dvpr (Addgene plasmid #8455) and pCMV-VSVG (Addgene plasmid #8454). For the official vector of The RNAi Consortium and a plasmid map, please see plasmid #10878.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Lentivirus-delivered stable gene silencing by RNAi in primary cells. Stewart et al (RNA 2003 Apr;9(4):493-501. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8453" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only