Addgene - pLKO.1 puro Plasmid Data

Plasmid Cart

Login to add to cart

Recently Visited

8453: pLKO.1 puro
8408: p255 pPr-CRE…
8401: p224 pCMV-RF…
8396: p218 pCMV-CR…
8395: p210 pCMV-CR…

Popular Plasmids

Special Collections

Analyze Sequence

Vector Database

Plasmid 8453: pLKO.1 puro

Gene/insert name:
None
Alt name:
LKO.1
Vector backbone:
pLKO.1
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, RNAi
Backbone size w/o insert:
7032
Cloning site 5':
AgeI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
LKO.1 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (2)
Supplemental document:
Addgene's pLKO.1 protocol
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

Empty lentiviral vector for siRNA expression; replaces Lentihair; see author's map. The 293T cell line can be obtained from the Weinberg lab or GenHunter http://genhunter.com/products/aptag-3/index.html

For packaging, please use pCMV-dR8.2 dvpr (Addgene plasmid #8455) and pCMV-VSVG (Addgene plasmid #8454). For the official vector of The RNAi Consortium and a plasmid map, please see plasmid #10878.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Lentivirus-delivered stable gene silencing by RNAi in primary cells. Stewart et al (RNA 2003 Apr;9(4):493-501. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8453" in your Materials and Methods section.