Addgene - pCMV-dR8.2 dvpr Plasmid Data

Plasmid 8455: pCMV-dR8.2 dvpr

Gene/insert name:
None
Alt name:
pHR' CMV 8.2 deltaR
Alt name:
pCMV-dR8.2 dvpr
Alt name:
pCMVdR8.2 dvpr
Vector backbone:
na
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral
5' sequencing primer:
CMV Forward List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (4)
Supplemental document:
Weinberg-viral (application/pdf)
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

Packaging plasmid for Lentiviral constructs (vpr is removed). The 293T cell line can be obtained from the Weinberg lab or GenHunter http://genhunter.com/products/aptag-3/index.html

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Lentivirus-delivered stable gene silencing by RNAi in primary cells. Stewart et al (RNA 2003 Apr;9(4):493-501. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8455" in your Materials and Methods section.