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(Plasmid #12253)

  • Purpose
    3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)
  • Depositing Lab
  • Price
    $65 (USD)
    Shipped as bacteria in an agar stab at ambient temperature
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Available to Academic and Nonprofits Only


  • Vector backbone
  • Backbone size w/o insert (bp) 4174
  • Vector type
    Mammalian Expression, Lentiviral ; Packaging

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Sequence Information


  • Gene/Insert name
  • Entrez Gene
    rev (a.k.a. HIV1gp6)

Cloning Information

Resource Information

Depositor Comments

Rev cDNA expressing plasmid in which the joined second and third exons of HIV-1 rev are under the transcriptional control of RSV U3 promoter.

Part of the 3rd generation packaging system. Please note that most of Trono lab lentiviral vectors can only be used with the 2nd generation packaging system. See notes for pMDLg/pRRE.

Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab for cloning strategies, protocols, publications, and more. See LentiWeb for discussions on cloning strategies and protocols.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSV-Rev was a gift from Didier Trono (Addgene plasmid # 12253)
  • For your References section:

    A third-generation lentivirus vector with a conditional packaging system. Dull T, Zufferey R, Kelly M, Mandel RJ, Nguyen M, Trono D, Naldini L. J Virol. 1998 Nov . 72(11):8463-71. PubMed 9765382