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Plasmid 12253: pRSV-Rev
  • 3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)

  • Rev

  • rev (HIV1gp6)

  • pRSV-Rev
    (Search Vector Database)

  • Mammalian Expression, Lentiviral ; Packaging

  • 4174

  • pREP fwd List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (5)
  • View map

  • Didier Trono

  • UBMTA

Comments: 

Rev cDNA expressing plasmid in which the joined second and third exons of HIV-1 rev are under the transcriptional control of RSV U3 promoter.

Part of the 3rd generation packaging system. Please note that most of Trono lab lentiviral vectors can only be used with the 2nd generation packaging system. See notes for pMDLg/pRRE.

Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussions on cloning strategies and protocols.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A third-generation lentivirus vector with a conditional packaging system. Dull et al (J Virol. 1998 Nov . 72(11):8463-71. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12253" in your Materials and Methods section.