Purpose3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12253||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4174
Vector typeMammalian Expression, Lentiviral ; Packaging
Growth in Bacteria
Copy numberHigh Copy
Entrez Generev (a.k.a. HIV1gp6)
- Cloning method Restriction Enzyme
- 5′ sequencing primer pREP fwd (Common Sequencing Primers)
Rev cDNA expressing plasmid in which the joined second and third exons of HIV-1 rev are under the transcriptional control of RSV U3 promoter.
Part of the 3rd generation packaging system. Please note that most of Trono lab lentiviral vectors can only be used with the 2nd generation packaging system. See notes for pMDLg/pRRE.
Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussions on cloning strategies and protocols.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRSV-Rev was a gift from Didier Trono (Addgene plasmid # 12253)
For your References section:A third-generation lentivirus vector with a conditional packaging system. Dull T, Zufferey R, Kelly M, Mandel RJ, Nguyen M, Trono D, Naldini L. J Virol. 1998 Nov . 72(11):8463-71. PubMed 9765382