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pMD2.G Notes

Addgene diagnostic digest- NotI, HindIII

This gel depicts the NEB 1kb ladder and a digestion of the plasmid with the following enzymes: NotI, HindIII and NotI+HindIII double digest.

We expected the following band sizes for this digest: 5.8kb; 5.8kb; 3kb and 2.8kb. We see the expected pattern.

Addgene Diagnostic Digest- BamHI

This gel depicts the NEB 1kb ladder and a digestion of the plasmid pMD2.G with the following enzymes: BamHI-HF.

Expected Bands
BamHI-HF : 5 kb, .8 kb & .031 kb (off gel)

We observe the expected bands for this digest.

Addgene Diagnostic Digest- PvuI-HF and ApaI

This gel depicts the NEB 1kb ladder and a digestion of the plasmid pMD2.G with the following enzymes: PvuI-HF and ApaI.

Expected Bands:
PvuI-HF: 5822 bp
ApaI: 5822 bp

We observe the expected bands for this digest.

Addgene Diagnostic Digest- EcoRI

This gel depicts the NEB 1kb ladder and a digestion of the plasmid pMD2.G with the following enzymes: EcoRI-HF.

Expected bands
EcoRI-HF: 4154 bp & 1668 bp

We observe the expected bands for this digest.