Addgene - pBABE-puro Plasmid Data

Plasmid 1764: pBABE-puro

Gene/insert name:
None
Vector backbone:
pBABE-puro
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size (bp):
5169
5' sequencing primer:
pBABE 5' List of Sequencing Primers
3' sequencing primer:
pBABE 3'
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (3)
Map:
View map
Supplemental document:
pBABE protocol (application/pdf)
Supplemental document:
Notes from Addgene (2)
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

Morgenstern JP, Land H., 1990, Nucleic Acids Research 18(12):3587-96.

If you are using the pBABE protocol from the Weinberg Lab to generate virus, please note that the Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging. The pCL-Eco plasmid listed in their protocol should be substituted with pUMVC.

Please see "Notes from Addgene" for the best sequence of the MCS region.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1764" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

Print

This is commonly requested with