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Plasmid 1764: pBABE-puro
  • None

  • pBABE-puro
    (Search Vector Database)

  • Mammalian Expression, Retroviral

  • 5169

  • pBABE 5' List of Sequencing Primers

  • pBABE 3'

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • Puromycin

  • View sequences (4)
  • View map

  • pBABE protocol (application/pdf)

  • Notes from Addgene (2)

  • Bob Weinberg

  • UBMTA

Comments: 

Morgenstern JP, Land H., 1990, Nucleic Acids Research 18(12):3587-96.

If you are using the pBABE protocol from the Weinberg Lab to generate virus, please note that the Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging. The pCL-Eco plasmid listed in their protocol should be substituted with pUMVC.

Please see "Notes from Addgene" for the best sequence of the MCS region.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1764" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only