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Plasmid 1764: pBABE-puro
  • Retroviral vector for cloning and expressing your gene of interest. Puromycin selection.

  • None

  • pBABE-puro
    (Search Vector Database)

  • Mammalian Expression, Retroviral

  • 5169

  • pBABE 5' List of Sequencing Primers

  • pBABE 3'

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • Puromycin

  • View sequences (4)
  • View map

  • pBABE protocol (application/pdf)

  • Notes from Addgene (2)

  • Bob Weinberg

  • UBMTA

Comments: 

Morgenstern JP, Land H., 1990, Nucleic Acids Research 18(12):3587-96.

If you are using the pBABE protocol from the Weinberg Lab to generate virus, please note that the Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging. The pCL-Eco plasmid listed in their protocol should be substituted with pUMVC.

Please see "Notes from Addgene" for the best sequence of the MCS region.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1764" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only