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pMKO.1 GFP Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid with the following enzyme: MluI

We expected the following band sizes for this digest: 6.7kb (linearization). We see the expected pattern.

Addgene diagnostic digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid with the following enzyme: AgeI

We expected the following band sizes for this digest: 6.7kb (linearization). We see the expected pattern.

Diagnostic digest 3

This gel depicts the NEB 1kb ladder and a digestion of the plasmid with the following enzyme: EcoRI, XhoI and EcoRI+AgeI. We expected the following band sizes for this digest: 6.7kb, 6.7kb and 6.7kb+0.03kb. We see the expected pattern.