This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

CRISPR Plasmids: Base Edit


Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a specific base editor that can alter DNA bases without inducing a DNA break. Base editors convert C->T (or G->A on the opposite strand) within a small editing window specified by the gRNA. CRISPR-X, a base-editing system from the Bassik lab, uses an MS2 hairpin-tagged gRNA to recruit a cytidine deaminase and induce somatic hypermutation in a 100 bp window. This technique creates diverse populations of mutants for directed evolution. If you’re interested in making specific modifications to a gene rather than creating loss-of-function frameshift alleles, base editors may be useful in your research.

CRISPR base editing schematic

Browse, sort, or search the tables below for CRISPR base editing plasmids.
Plasmids are available for expression in mammalian systems, bacteria, plants, and yeast.


Mammalian

Plasmid Gene/Insert Promoter Selectable Marker PI Publication Hidden Extra Search Info
pCMV-BE1BE1 (Rattus norvegicus)CMV Liu Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature. 2016 Apr 20. doi: 10.1038/nature17946. Expresses BE1 with C-terminal NLS in mammalian cells CMV
pCMV-BE2BE2 (Rattus norvegicus)CMV Liu Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature. 2016 Apr 20. doi: 10.1038/nature17946. Expresses BE2 in mammalian cells CMV
pCMV-BE3BE3 (Rattus norvegicus)CMV Liu Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature. 2016 Apr 20. doi: 10.1038/nature17946. Expresses BE3 in mammalian cells CMV
MO91-dCas9-AID(P182X)dCas9-AID P182X (Synthetic)MSCVPuromycin Chang Targeted AID-mediated mutagenesis (TAM) enables efficient genomic diversification in mammalian cells. Nat Methods. 2016 Dec;13(12):1029-1035. doi: 10.1038/nmeth.4027. Epub 2016 Oct 10. Expresses fusion of human codon-optimized S. pyogenes dCas9 (D10A, H840A ) protein with a human AID mutant protein (p182X). MSCV Retroviral backbone. M091
pJL-SaBE3SaBE3 (Rattus norvegicus)CMVNeomycin (select with G418) Liu Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. Expresses SaBE3 in mammalian cells CMV
pJL-SaKKH-BE3SaKKH-BE3 (Rattus norvegicus)CMV Liu Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. Expresses SaKKH-BE3 in mammalian cells [Edit] CMV
pBK-VQR-BE3VQR-BE3 (Rattus norvegicus)CMVNeomycin (select with G418) Liu Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. Expresses VQR-BE3 in mammalian cells CMV
pBK-EQR-BE3EQR-BE3 (Rattus norvegicus)CMVNeomycin (select with G418) Liu Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. Expresses EQR-BE3 in mammalian cells CMV
pBK-VRER-BE3VRER-BE3 (Rattus norvegicus)CMVNeomycin (select with G418) Liu Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. Expresses VRER-BE3 in mammalian cells CMV
pBK-YE1-BE3YE1-BE3 (Rattus norvegicus)CMVNeomycin (select with G418) Liu Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. Expresses YE1-BE3 in mammalian cells CMV
pBK-EE-BE3EE-BE3 (Rattus norvegicus)CMVNeomycin (select with G418) Liu Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. Expresses EE-BE3 in mammalian cells CMV
pBK-YE2-BE3YEE-BE3 (Rattus norvegicus)CMVNeomycin (select with G418) Liu Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. Expresses YE2-BE3 in mammalian cells CMV
pBK-YEE-BE3YEE-BE3 (Rattus norvegicus)CMVNeomycin (select with G418) Liu Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. Expresses YEE-BE3 in mammalian cells CMV
pGH335_MS2-AID*Δ-HygroMS2-AID*Δ and Hygromycin resistanceHygromycin Bassik Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038. lentiviral expression vector for MS2-AID*Δ fusion protein with hygromycin B selectable marker Addgene #61426
pGH183_MS2-AIDΔDead-HygroMS2-AIDΔDead and Hygromycin resistanceHygromycin Bassik Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038. lentiviral expression vector for MS2-AIDΔDead fusion protein with hygromycin B selectable marker Addgene #61426
pGH156_MS2-AID-HygroMS2-AID and Hygromycin resistanceHygromycin Bassik Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038. lentiviral expression vector for MS2-AID fusion protein with hygromycin B selectable marker Addgene #61426
pGH153_MS2-AIDΔ-HygroMS2-AIDΔ and Hygromycin resistanceHygromycin Bassik Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038. lentiviral expression vector for MS2-AIDΔ fusion protein with hygromycin B selectable marker Addgene #61426
pCMV-HF-BE3HF-BE3 (Homo sapiens)CMV Liu Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery. Nat Commun. 2017 Jun 6;8:15790. doi: 10.1038/ncomms15790. Expresses HF-BE3 with C-terminal NLS in mammalian cells pCMV
pET-Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLSrAPOBEC1-nCas9 (D10A)-UGI-NLS (Rattus norvegicus)T7 Kim Highly efficient RNA-guided base editing in mouse embryos. Nat Biotechnol. 2017 Feb 27. doi: 10.1038/nbt.3816. Expression of Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS in bacterial cells. pET28a
BE4BE (Rattus norvegicus)CMV Liu Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. Expresses BE4 in mammalian cells pCMV
AID-BE3AID-BE3 (Homo sapiens)CMV Liu Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. Expresses AID-BE3 in mammalian cells pCMV
CDA1-BE3CDA1-BE3 (Synthetic)CMV Liu Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. Expresses CDA1-BE3 in mammalian cells pCMV
SaBE4SaBE4 (Rattus norvegicus)CMV Liu Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. Expresses SaBE4 in mammalian cells pCMV
BE4-GamBE4-Gam (Rattus norvegicus)CMV Liu Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. Expresses BE4-Gam in mammalian cells pCMV
BE3-GamBE3-Gam (Rattus norvegicus)CMV Liu Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. Expresses BE3-Gam in mammalian cells pCMV
SaBE4-GamSaBE4-Gam (Rattus norvegicus)CMV Liu Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. Expresses SaBE4-Gam in mammalian cells pCMV
SaBE3-GamSaBE3-Gam (Rattus norvegicus)CMV Liu Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. Expresses SaBE3-Gam in mammalian cells pCMV
pCMV-eBE-S1eBE-S1 (Rattus norvegicus)CMV Chen Enhanced base editing by co-expression of free uracil DNA glycosylase inhibitor. Cell Res. 2017 Aug 29. doi: 10.1038/cr.2017.111. Expresses eBE-S1 in mammalian cells pCMV
pCMV-eBE-S3eBE-S3 (Rattus norvegicus)CMV Chen Enhanced base editing by co-expression of free uracil DNA glycosylase inhibitor. Cell Res. 2017 Aug 29. doi: 10.1038/cr.2017.111. Expresses eBE-S3 in mammalian cells pCMV
pCMV-ABE6.3ABE6.3 (Synthetic)CMV Liu Programmable base editing of A*T to G*C in genomic DNA without DNA cleavage. Nature. 2017 Nov 23;551(7681):464-471. doi: 10.1038/nature24644. Epub 2017 Oct 25. expresses ABE6.3 in mammalian cells pBR322/CMV
pCMV-ABE7.8ABE7.8 (Synthetic)CMV Liu Programmable base editing of A*T to G*C in genomic DNA without DNA cleavage. Nature. 2017 Nov 23;551(7681):464-471. doi: 10.1038/nature24644. Epub 2017 Oct 25. expresses ABE7.8 in mammalian cells pBR322/CMV
pCMV-ABE7.9ABE7.9 (Synthetic)CMV Liu Programmable base editing of A*T to G*C in genomic DNA without DNA cleavage. Nature. 2017 Nov 23;551(7681):464-471. doi: 10.1038/nature24644. Epub 2017 Oct 25. expresses ABE7.9 in mammalian cells pBR322/CMV
pCMV-ABE7.10ABE7.10 (Synthetic)CMV Liu Programmable base editing of A*T to G*C in genomic DNA without DNA cleavage. Nature. 2017 Nov 23;551(7681):464-471. doi: 10.1038/nature24644. Epub 2017 Oct 25. expresses ABE7.10 in mammalian cells pBR322/CMV

Bacteria

Plasmid Gene/Insert Promoter Selectable Marker PI Publication
pET42b-BE3BE3 (Homo sapiens)T7 Liu Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery. Nat Commun. 2017 Jun 6;8:15790. doi: 10.1038/ncomms15790.
pET42b-HF-BE3HF-BE3 (Homo sapiens) Liu Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery. Nat Commun. 2017 Jun 6;8:15790. doi: 10.1038/ncomms15790.
pET28b-BE3∆UGIBE3∆UGI (Rattus norvegicus)T7 Kim Genome-wide target specificities of CRISPR RNA-guided programmable deaminases. Nat Biotechnol. 2017 May;35(5):475-480. doi: 10.1038/nbt.3852. Epub 2017 Apr 10.

Plant

Plasmid Gene/Insert Promoter Selectable Marker PI Publication Hidden Extra Search Info
pMonAID_dCas9-PmCDA_Hyg_ALSSpCas9 (Other)2x35SHygromycin Kondo Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion. Nat Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3833. Monocot Target-AID vector expressing rice-optimized dCas9-PmCDA1 with sgRNA targeting OsAls pZDgRNA_Cas9ver.2 (D10A)_HPT
pMonAID_nCas9-PmCDA_Hyg_ALSSpCas9 (Other)2x35SHygromycin Kondo Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion. Nat Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3833. Monocot Target-AID vector expressing rice-optimized nCas9-PmCDA1 with sgRNA targeting OsAls pZDgRNA_Cas9ver.2 (D10A)_HPT
pDicAID_nCas9-PmCDA_NptII_DellaSpCas9 (Other)PcUbiKanamycin Kondo Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion. Nat Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3833. Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1 with sgRNA targeting SlDella pZD_OsU3gYSA_HolgerCas9_NPTII
pDicAID_nCas9-PmCDA-2A-NptII_ETRSpCas9 (Other)PcUbiKanamycin Kondo Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion. Nat Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3833. Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1-2A-NptII with sgRNA targeting SlEtr pZD_OsU3gYSA_HolgerCas9_NPTII
pHEE901Hygromycin Chen CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5. CRISPR/Cas9-mediated base editing in Arabidopsis. Contains Cas9(D10A) and empty gRNA scaffold, HygR pCambia
pHSE901Hygromycin Chen CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5. CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, HygR pCambia
pBSE901Neomycin (select with G418) Chen CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5. CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta resistance pCambia
pXSE901BBasta Chen CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5. CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Bar resistance pCambia
pXSE901BGBasta ; Glyphosate Chen CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5. CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta and glyphosate-resistance pCambia
pB-dCas9-PBENLS-APOBEC1-XTEN-dCas9-UGI-NLSUbiBasta Gao Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27. Expresses CRISPR base editor in maize pBUE411
pB-nCas9-PBENLS-APOBEC1-XTEN-nCas9-UGI-NLSUbiBasta Gao Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27. Expresses CRISPR base editor in maize pBUE411
pdCas9-PBENLS-APOBEC1-XTEN-dCas9-UGI-NLSUbi Gao Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27. Expresses CRISPR base editor in protoplasts pJIT163-Ubi-GFP
pH-dCas9-PBENLS-APOBEC1-XTEN-dCas9-UGI-NLSUbiHygromycin Gao Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27. Expresses CRISPR base editor in rice pHUE411
pH-nCas9-PBENLS-APOBEC1-XTEN-nCas9-UGI-NLSUbiHygromycin Gao Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27. Expresses CRISPR base editor in rice pHUE411
pnCas9-PBENLS-APOBEC1-XTEN-nCas9-UGI-NLSUbi Gao Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27. Expresses CRISPR base editor in protoplasts pJIT163-Ubi-GFP

Yeast

Plasmid Gene/Insert Promoter Selectable Marker PI Publication Hidden Extra Search Info
pRS315e_pGal-dCas9-SH3-pGal-PmCDA1-SHLSpCas9 (Other), PmCDA1 (Other)pGal1, pGal10LEU2 Kondo Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems. Science. 2016 Aug 4. pii: aaf8729. Expresses dCas9-SH3 and PmCDA1-SHL in yeast cells pRS315
pRS315e_pGal-dCas9-PmCDA1SpCas9 (Other)pGal1LEU2 Kondo Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems. Science. 2016 Aug 4. pii: aaf8729. Expresses dCas9-PmCDA1 in yeast cells pRS315
pRS315e_pGal-nCas9(D10A)-PmCDA1SpCas9 (Other)pGal1LEU2 Kondo Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems. Science. 2016 Aug 4. pii: aaf8729. Expresses nCas9(D10A)-PmCDA1 in yeast cells pRS315


Addgene Blugene icon

Do you have suggestions for other plasmids that should be added to this list?

Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource!