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CRISPR Plasmids: Base Edit


Two classes of base editors--cytosine base editors (CBEs) and adenine base editors (ABEs)--can be used to generate single base pair edits without double stranded breaks.

Cytosine base editors are created by fusing Cas9 nickase or catalytically inactive “dead” Cas9 (dCas9) to a cytidine deaminase like APOBEC. Base editors are targeted to a specific locus by a gRNA, and they can convert cytidine to uridine within a small editing window near the PAM site. Uridine is subsequently converted to thymidine through base excision repair, creating a C to T change (or a G to A on the opposite strand.)

Likewise, adenine base editors have been engineered to convert adenosine to inosine, which is treated like guanosine by the cell, creating an A to G (or T to C) change. Adenine DNA deaminases do not exist in nature, but have been created by directed evolution of the Escherichia coli TadA, a tRNA adenine deaminase. Like cytosine base editors, the evolved TadA domain is fused to a Cas9 protein to create the adenine base editor.

Both types of base editors are available with multiple Cas9 variants including high fidelity Cas9’s. Further advancements have been made by optimizing expression of the fusions, modifying the linker region between Cas variant and deaminase to adjust the editing window, or adding fusions that increase product purity such as the DNA glycosylase inhibitor (UGI) or the bacteriophage Mu- derived Gam protein (Mu- GAM).

While many base editors are designed to work in a very narrow window proximal to the PAM sequence, some base editing systems create a wide spectrum of single-nucleotide variants (somatic hypermutation) in a wider editing window, and are thus well suited to directed evolution applications. Examples of these base editing systems include targeted AID-mediated mutagenesis (TAM) from the Chang lab and CRISPR-X from the Bassik lab, in which Cas9 is fused to activation-induced cytidine deaminase (AID).

CRISPR base editing schematic


Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations, and more, created and maintained by the Doudna Lab.

Browse, sort, or search the tables below for CRISPR base editing plasmids.
Plasmids are available for expression in mammalian systems, bacteria, plants, yeast, and zebrafish.


Mammalian

Plasmid Gene/Insert Promoter Selectable Marker PI Publication

Bacteria

Plasmid Gene/Insert Promoter Selectable Marker PI Publication

Plant

Plasmid Gene/Insert Promoter Selectable Marker PI Publication

Yeast

Plasmid Gene/Insert Promoter Selectable Marker PI Publication

Zebrafish

Plasmid Gene/Insert Promoter Selectable Marker PI Publication


Do you have suggestions for other plasmids that should be added to this list?

Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource!