Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

CRISPR Plasmids: Activate

Browse CRISPR Plasmids

By Function
Genome Editing
Transcriptional Regulation
RNA Targeting
Other Applications
Screen
gRNAs
By Species

CRISPR Resources

Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest. If the plasmid that you choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator to your specific locus.


CRISPR-activation3-horizontal.png

Browse, sort, or search the tables below for CRISPR plasmids for transcriptional activation.
Plasmids are available for expression in mammalian systems, bacteria, Drosophila, plants, and yeast.

Mammalian

Plasmid Gene/Insert Promoter Selectable Marker PI Publication Hidden Extra Search Info
pMSCV-LTR-dCas9-VP64-BFPdCas9-VP64-BFP fusion (Homo sapiens), Puromycin resistanceLTR, PGKPuromycin Qi CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044. Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, VP64 and tagBFP MSCV-puro
pMSCV-LTR-dCas9-p65AD-BFPdCas9-p65AD-BFP fusion (Homo sapiens), Puromycin resistanceLTR, PGKPuromycin Qi CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044. Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, p65 activation domain and tagBFP MSCV-puro
pcDNA-dCas9-VP64dCas9-VP64 (Other)CMV Gersbach RNA-guided gene activation by CRISPR-Cas9-based transcription factors. Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2600. Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cells pcDNA3.1
Cas9m2-VP64Cas9m2-VP64 (Other) Church CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675. Cas9m2 Activator pcDNA3.3_TOPO
Cas9m3-VP64Cas9m3-VP64 (Other) Church CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675. Cas9m3 Activator pcDNA3.3_TOPO
Cas9m4-VP64Cas9m4-VP64 (Other) Church CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675. Cas9m4 Activator pcDNA3.3_TOPO
pSL690dCas9-VP64 (Synthetic)CMV Joung CRISPR RNA-guided activation of endogenous human genes. Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2598. Expresses dCas9-VP64 fusion unknown
pMLM3705codon optimized dCas9-VP64 (Synthetic)CMV Joung CRISPR RNA-guided activation of endogenous human genes. Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2598. Expresses mammalian cell codon-optimized dCas9-VP64 pJDS246
pAC1-pCR8-dCas9VP48dCas9(D10A;H840A) fusion with VP48 activation domain (Synthetic) Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. dCas9VP48 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression pCR8/GW/TOPO
pAC147-pCR8-dCas9VP64dCas9(D10A;H840A) fusion with VP64 activation domain (Homo sapiens) Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. dCas9VP64 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression  pCR8/GW/TOPO
pAC148-pCR8-dCas9VP96dCas9(D10A;H840A) fusion with VP96 activation domain (Homo sapiens) Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. dCas9VP96 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression pCR8/GW/TOPO
pAC149-pCR8-dCas9VP160dCas9(D10A;H840A) fusion with VP160 activation domain (Homo sapiens) Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. dCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression pCR8/GW/TOPO
pAC91-pmax-dCas9VP64dCas9(D10A;H840A) fusion with VP64 activation domain (Homo sapiens)CAGGS Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. dCas9VP64 on pmax expression vector pmax-DEST (Addgene: 48222)
pAC92-pmax-dCas9VP96dCas9(D10A;H840A) fusion with VP96 activation domain (Homo sapiens)CAGGS Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. dCas9VP96 on pmax expression vector pmax-DEST (Addgene: 48222)
pAC93-pmax-dCas9VP160dCas9(D10A;H840A) fusion with VP160 activation domain (Homo sapiens)CAGGS Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. dCas9VP160 on pmax expression vector pmax-DEST (Addgene: 48222)
pAC94-pmax-dCas9VP160-2A-purodCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro (Homo sapiens)CAGGSPuromycin Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested. pmax-DEST (Addgene: 48222)
pAC95-pmax-dCas9VP160-2A-neodCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-neo (Homo sapiens)CAGGSNeomycin (select with G418) Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. dCas9VP160-2A-neo (neo/G418-selectable) on pmax expression vector. Note: This is being tested. pmax-DEST (Addgene: 48222)
pAC2-dual-dCas9VP48-sgExpressiondCas9VP48 (Homo sapiens) Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. Dual expression construct expressing both dCas9VP48 and sgRNA from separate promoters pX335 (Addgene #42335)
pAC5-dual-dCas9VP48-sgTetOdCas9VP48 and sgTetO (Synthetic) Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. Dual expression construct expressing both dCas9VP48 and sgTetO from separate promoters pAC2-dual-dCas9VP48-sgExpression (Addgene #48236)
pAC152-dual-dCas9VP64-sgExpressiondCas9 (Synthetic) Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. Dual expression construct expressing both dCas9VP64 and sgRNA from separate promoters pX335 (Addgene #42335)
  • Tags / Fusion Proteins
    • HA-Tag (N terminal on insert)
    • VP64 (C terminal on insert)
    		•
    pAC153-dual-dCas9VP96-sgExpressiondCas9 (Synthetic) Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. Dual expression construct expressing both dCas9VP96 and sgRNA from separate promoters pX335 (Addgene #42335)
  • Tags / Fusion Proteins
    • HA-Tag (N terminal on insert)
    • VP96 (C terminal on insert)
    		•
    pAC154-dual-dCas9VP160-sgExpressiondCas9 (Synthetic) Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. Dual expression construct expressing both dCas9VP160 and sgRNA from separate promoters pX335 (Addgene #42335)
  • Tags / Fusion Proteins
    • HA-Tag (N terminal on insert)
    • VP160 (C terminal on insert)
    		•
    M-SPn-VP64Cas9-VP64, nuclease-null (Other)CMVNeomycin (select with G418) Church Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681. Mammalian SP-VP64 nuclease-null Cas9 activator expression, human optimized pcDNA3.3 TOPO
    M-ST1n-VP64Cas9-VP64, nuclease-null (Other)CMVNeomycin (select with G418) Church Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681. Mammalian ST1-VP64 nuclease-null Cas9 activator expression, human optimized pcDNA3.3 TOPO
    M-NMn-VP64Cas9-VP64, nuclease-null (Other)CMVNeomycin (select with G418) Church Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681. Mammalian NM-VP64 nuclease-null Cas9 activator expression, human optimized pcDNA3.3 TOPO
    pCMV_dCas9_VP64dCas9_VP64 (human-codon-optimized) (Homo sapiens)CMV Lu Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11. encodes human-optimized dCas9_VP64 synthetic transcription factor phi-Yellow-Dest
    pHAGE TRE dCas9-VP64dCas9 (Other)TRENeomycin (select with G418) Wolfe Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341. Tet-regulatable dCas9-VP64 2nd generation lentiviral expression vector pHAGE
    pHAGE EF1α dCas9-VP64dCas9 (Other)EF1alphaPuromycin Wolfe Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341. Constitutive dCas9-VP64 lentiviral expression vector pHAGE
    pLV hUbC-dCas9 VP64-T2A-GFPhumanized dead Cas9 VP64 T2A GFP (Other)hUbC Gersbach Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Res. 2014 Aug 13. pii: gku749. Co-expresses human optimized S. pyogenes dCas9-VP64 and GFP FUGW
    CMVp-dCas9-3xNLS-VP64 (Construct 1)dCas9 (Homo sapiens)CMV/hUBC Lu Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells. Mol Cell. 2014 May 14. pii: S1097-2765(14)00355-4. doi: 10.1016/j.molcel.2014.04.022. Expresses taCas9 in Mammalian cells for transactivating endogenous and synthetic promoters. The backbone is a lentiviral vector. pFUGw (Addgene id: 25870)
    pLV hUbC-VP64 dCas9 VP64-T2A-GFPhumanized VP64 dead Cas9 VP64 T2A GFP (Other) Gersbach Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Res. 2014 Aug 13. pii: gku749. Co-expresses human optimized S. pyogenes dCas9 fused to two copies of VP64 and GFP FUGW
    pcDNA3.1-CibN-dCas9CibN-dCas9 (Arabidopsis thaliana)CMVNeomycin (select with G418) Gersbach A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753. Expresses CibN-dCas9 in mammalian cells pcDNA3.1
    pcDNA3.1-dCas9-CibNdCas9-CibN (Arabidopsis thaliana)CMVNeomycin (select with G418) Gersbach A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753. Expresses dCas9-CibN in mammalian cells pcDNA3.1
    pcDNA3.1-CibN-dCas9-CibNdCas9-CibN (Arabidopsis thaliana)CMVNeomycin (select with G418) Gersbach A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753. Expresses CibN-dCas9-CibN in mammalian cells pcDNA3.1
    pHRdSV40-dCas9-10xGCN4_v4-P2A-BFPCas9 dead Vale A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging. Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039. Expressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activation pHR
    pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLSscFv-GCN4 Vale A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging. Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039. The plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64 pHR
    pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPREdCas9dSV40 Promoter Vale A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging. Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039. dCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag system pHR
    dCAS9-VP64_GFPdCAS9(D10A,H840A)-VP64_2A_GFP (Synthetic)EF1AGFP Zhang Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature. 2014 Dec 10. doi: 10.1038/nature14136. Expresses dCAS9-VP64 activator with 2A GFP lenti(AMP)
    lenti dCAS-VP64_BlastdCAS9(D10A, N863A)-VP64_2A_Blast (Synthetic)EF1ABlasticidin Zhang Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature. 2014 Dec 10. doi: 10.1038/nature14136. 3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE) plenti
    TetO-FUW-VdC9BVVP64dCas9BFPVP64 (Synthetic) Leong A CRISPR/Cas9-Based System for Reprogramming Cell Lineage Specification. Stem Cell Reports. 2014 Dec 9;3(6):940-7. doi: 10.1016/j.stemcr.2014.09.013. Epub 2014 Oct 23. Expresses RNA-Guided, Nuclease-Inactive VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation of Endogenous Genes TetO-FUW
    pJZC32sgRNA, MCP-VP64U6, CMVMarked by mCherry Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA (no RNA aptamer addition) with MCP-VP64 effector for mammalian cells MP177_U6 (derived from pSico)
    pJZC25sgRNA + 1x MS2 binding module, MCP-VP64U6, CMVMarked by mCherry Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA + 1x MS2 with MCP-VP64 effector for mammalian cells MP177_U6 (derived from pSico)
    pJZC33sgRNA + 2x MS2 binding module, MCP-VP64U6, CMVMarked by mCherry Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA + 2x MS2 with MCP-VP64 effector for mammalian cells MP177_U6 (derived from pSico)
    pJZC34sgRNA + 2x MS2(wt+f6) binding module, MCP-VP64U6, CMVMarked by mCherry Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells MP177_U6 (derived from pSico)
    pJZC41sgRNA, PCP-VP64U6, CMVMarked by mCherry Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA (no RNA aptamer addition) with PCP-VP64 effector for mammalian cells MP177_U6 (derived from pSico)
    pJZC39sgRNA + 1x PP7, mCherryU6, CMVMarked by mCherry Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA + 1x PP7 with mCherry for mammalian cells MP177_U6 (derived from pSico)
    pJZC101sgRNA, COM-VP64U6, CMVMarked by mCherry Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA (no RNA aptamer addition) with COM-VP64 effector for mammalian cells MP177_U6 (derived from pSico)
    pJZC48sgRNA + 1x COM binding module, COM-VP64U6, CMVMarked by mCherry Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA + 1x COM with COM-VP64 effector for mammalian cells MP177_U6 (derived from pSico)
    pJZC116sgRNA + 2x MS2 (wt+f6) binding module, MCP-VP64U6, CMVMarked by BFP Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells, marked by BFP MP177_U6 (derived from pSico)
    PX855SpCas9 (aa 2-535)CBh Zhang A split-Cas9 architecture for inducible genome editing and transcription modulation. Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149. N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES) PX330
    PX856SpCas9 (aa536-1368)CBh Zhang A split-Cas9 architecture for inducible genome editing and transcription modulation. Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149. C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64) PX330
    px330-CIB1-dCas9U6 and CMV Kong Aspirin cooperates with p300 to activate the acetylation of H3K9 and promote FasL-mediated apoptosis of cancer stem-like cells in colorectal cancer Theranostics Mammalian gRNA expression vector also expressing CIB1-dCas9 px330
    SP-dCas9-VPRSP-dCas9-VPR (Homo sapiens)CMVNeomycin (select with G418) Church Highly efficient Cas9-mediated transcriptional programming. Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312. SP-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; mammalian vector pcDNA3.3 TOPO
    M_ST1n_VPRST1-dCas9-VPR (Homo sapiens)CMVNeomycin (select with G418) Church Highly efficient Cas9-mediated transcriptional programming. Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312. ST1-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; mammalian vector pcDNA3.3 TOPO
    PB-TRE-dCas9-VPRdCas9-VPR (Homo sapiens)TREHygromycin Church Highly efficient Cas9-mediated transcriptional programming. Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312. SP-dCas9-VPR with doxycycline-inducible expression PB-TRE
    NLS-dCas9-trCIB1dCas9-trCIB1 fusion (Synthetic)CMVNeomycin (select with G418) Sato CRISPR-Cas9-based Photoactivatable Transcription System. Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22. Photoactivatable transcription system. Expression of genomic anchor probe, containing dCas9 and CIB1 pcDNA3.1/V5-His A
    pJZC42sgRNA + 1XPP7, PCP-VP64 IRES mCherryU6, CMVmCherry fluorescence Lim Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA + 1XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry pSico
    pJZC43sgRNA + 2XPP7, PCP-VP64 IRES mCherryU6, CMVmCherry fluorescence Lim Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA + 2XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry pSico
    pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFPhU6-gRNA expression cassette (Synthetic), S.Pyogenes VP64-dCas9-VP64 (Other)hU6, hUbC Gersbach Multiplex CRISPR/Cas9 Genome Engineering for Directing Myogenic Cellular Differentiation in Primary Human Cells (unpublished) expresses a single gRNA and VP64-dCas9-VP64 FUGW
    pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)S.Pyogenes VP64-dCas9-VP64 (Other)hUbC Gersbach Multiplex CRISPR/Cas9 Genome Engineering for Directing Myogenic Cellular Differentiation in Primary Human Cells (unpublished) expresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removed FUGW
    pEF_dCas9-VP64dCas9 (Other)human EF1[alpha] Rinn Multiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1. Transient expression of Sp-dCas9 fused to the VP64 transcription activator, in mammalian cells, under an EF1-alpha promoter. pNEB193
  • Tags / Fusion Proteins
    • 3xFLAG (C terminal on insert)
    • VP64 (C terminal on insert)
    		•
    AAV_NLS-dSaCas9-NLS-VPRdSaCas9-VPR (Synthetic)CMV Church Cas9 gRNA engineering for genome editing, activation and repression. Nat Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580. AAV vector containing nuclease null SaCas9 fused to VPR pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA Plasmid #61592
    CAG-DDdCas9VP192-T2A-EGFP-ires-puroDD-dCas9VP192-T2A-EGFP (Other)CAGPuromycin Otonkoski Conditionally Stabilized dCas9 Activator for Controlling Gene Expression in Human Cell Reprogramming and Differentiation. Stem Cell Reports. 2015 Sep 8;5(3):448-59. doi: 10.1016/j.stemcr.2015.08.001. DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim. PyCAG
    pCXLE-dCas9VP192-T2A-EGFP-shP53dCas9-dCas9VP192-GFP-shp53 (Other)CAG Otonkoski Conditionally Stabilized dCas9 Activator for Controlling Gene Expression in Human Cell Reprogramming and Differentiation. Stem Cell Reports. 2015 Sep 8;5(3):448-59. doi: 10.1016/j.stemcr.2015.08.001. Episomal plasmid encoding dCas9VP192 and p53 shRNA pCXLE
    pCXLE-dCas9VP192-T2A-EGFPdCas9-dCas9VP192-EGFP (Other)CAG Otonkoski Conditionally Stabilized dCas9 Activator for Controlling Gene Expression in Human Cell Reprogramming and Differentiation. Stem Cell Reports. 2015 Sep 8;5(3):448-59. doi: 10.1016/j.stemcr.2015.08.001. Episomal plasmid encoding dCas9VP192 pCXLE
    pHRm-NLS-dCas9-GFP11x7-NLSNLS-dCas9-GFP11x7-NLS (Synthetic) Huang Versatile protein tagging in cells with split fluorescent protein. Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046. Expresses NLS-dCas9-GFP11x7-NLS in mammalian cells pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE (addgene #60910)
    pAC164-pmax-dCas9Master-VP64dCas9-VP64 (Synthetic)CAGGS + chim intron Cheng Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation and genomic labeling. Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 Jan 15. dCas9-3xGGGGS-VP64 in pmax expression vector pAC90-pmax-DEST
    pAC1364-pmax-dCas9Master_mCBPHATdCas9-mCBPHAT (Synthetic)CAGGS + chim intron Cheng Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation and genomic labeling. Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 Jan 15. dCas9Master_mCBPHAT in pmax expression vector pAC90-pmax-DEST
    pAC1410-pmax-dCas9Master_p65HSF1dCas9-p65HSF1 (Synthetic)CAGGS + chim intron Cheng Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation and genomic labeling. Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 Jan 15. dCas9Master_p65HSF1 in pmax expression vector pAC90-pmax-DEST
    lentiSAMv2U6 and EF1ABlasticidin Zhang Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening. Nat Protoc. 2017 Apr;12(4):828-863. doi: 10.1038/nprot.2017.016. Epub 2017 Mar 23. lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences. plenti
    AAVS1-idCas9-vprdCas9-VPR (Other)TRE-tightPuromycin Na An inducible CRISPR-ON system for controllable gene activation in human pluripotent stem cells. Protein Cell. 2017 Jan 23. doi: 10.1007/s13238-016-0360-8. inducible CRISPR-ON system for controllable gene activation; this plasmid is used to insert dCas9-VPR casette in one allele of AAVS1 locus AAVS1 homologous recombineering donor plasmid
    lentiSAM v2 (Puro)U6 (sgRNA) and EF1a (dCas9-VP64)Puromycin Karpf Lenti CRISPR Activate (unpublished) Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences. lentiSAM v2
    pXPR_120dCas9 (Other)EF1aBlasticidin Root Orthologous CRISPR-Cas9 enzymes for combinatorial genetic screens. Nat Biotechnol. 2018 Feb;36(2):179-189. doi: 10.1038/nbt.4048. Epub 2017 Dec 18. for CRISPRa, lentiviral expression of dCas9-VPR 2A BlastR pXPR
  • Tag / Fusion Protein
    • VPR (C terminal on insert)
    		•
    lenti-EF1a-dCas9-VP64-PurodCas9-VP64-T2A-Puro (Synthetic)EF-1aPuromycin, Zeocin Brennand Evaluating Synthetic Activation and Repression of Neuropsychiatric-Related Genes in hiPSC-Derived NPCs, Neurons, and Astrocytes. Stem Cell Reports. 2017 Aug 8;9(2):615-628. doi: 10.1016/j.stemcr.2017.06.012. Epub 2017 Jul 27. 3rd generation lenti vector encoding dCas9-VP64 with 2A puromycin resistance marker (EF1a-dCas9-VP64-T2A-Puro-WPRE) pLenti
    lenti-EF1a-dCas9-VPR-Puro(Sp)dCas9-VPR-P2A-Puro (Homo sapiens)EF-1aPuromycin, Zeocin Brennand Evaluating Synthetic Activation and Repression of Neuropsychiatric-Related Genes in hiPSC-Derived NPCs, Neurons, and Astrocytes. Stem Cell Reports. 2017 Aug 8;9(2):615-628. doi: 10.1016/j.stemcr.2017.06.012. Epub 2017 Jul 27. 3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE) pLenti
    pAAV-dSa-VPRdCas9 (Synthetic) Church Church Lab CRISPR plasmids 2017 (unpublished) Expresses tripartite VP64-p65-RTA activator fused to C term. of dead Sa Cas9 pAAV (pUC f1)
  • Tag / Fusion Protein
    • VP64-p65-RTA activator (C terminal on insert)
    		•
    dSp-p65 Full (1-261)dCas9 (Synthetic) Church Church Lab CRISPR plasmids 2017 (unpublished) Expresses dSp Cas9 fused to p65 activation domain pBR322
  • Tag / Fusion Protein
    • p65 activation domain (C terminal on insert)
    		•
    dSp-p65 (150-261)dCas9 (Synthetic) Church Church Lab CRISPR plasmids 2017 (unpublished) Expresses dSp Cas9 fused to truncated p65 activation domain pBR322
  • Tag / Fusion Protein
    • truncated p65 activation domain (150-261) (C terminal on insert)
    		•
    dSp-p65 (100-261)dCas9 (Synthetic) Church Church Lab CRISPR plasmids 2017 (unpublished) Expresses dSp Cas9 fused to truncated p65 activation domain pBR322
  • Tag / Fusion Protein
    • truncated p65 activation domain (100-261) (C terminal on insert)
    		•
    dSp-p65 (200-261)dCas9 (Synthetic) Church Church Lab CRISPR plasmids 2017 (unpublished) Expresses dSp Cas9 fused to truncated p65 activation domain pBR322
  • Tag / Fusion Protein
    • truncated p65 activation domain (200-261) (C terminal on insert)
    		•
    dSp-p65 (1-200)dCas9 (Synthetic) Church Church Lab CRISPR plasmids 2017 (unpublished) Expresses dSp Cas9 fused to truncated p65 activation domain pBR322
  • Tag / Fusion Protein
    • truncated p65 activation domain (1-200) (C terminal on insert)
    		•
    dSp-p65 (1-150)dCas9 (Synthetic) Church Church Lab CRISPR plasmids 2017 (unpublished) Expresses dSp Cas9 fused to truncated p65 activation domain pBR322
  • Tag / Fusion Protein
    • truncated p65 activation domain (1-150) (C terminal on insert)
    		•
    dSp-p65 (1-100)dCas9 (Synthetic) Church Church Lab CRISPR plasmids 2017 (unpublished) Expresses dSp Cas9 fused to truncated p65 activation domain pBR322
  • Tag / Fusion Protein
    • truncated p65 activation domain (1-100) (C terminal on insert)
    		•
    dSp-p65 (50-150)dCas9 (Synthetic) Church Church Lab CRISPR plasmids 2017 (unpublished) Expresses dSp Cas9 fused to truncated p65 activation domain pBR322
  • Tag / Fusion Protein
    • truncated p65 activation domain (50-150) (C terminal on insert)
    		•
    dSp-Rta Full (1-190)dCas9 (Synthetic) Church Church Lab CRISPR plasmids 2017 (unpublished) Expresses dSp Cas9 fused to truncated RTA activation domain pBR322
  • Tag / Fusion Protein
    • truncated RTA activation domain (1-190) (C terminal on insert)
    		•
    dSp-Rta 75-190dCas9 (Synthetic) Church Church Lab CRISPR plasmids 2017 (unpublished) Expresses dSp Cas9 fused to truncated RTA activation domain pBR322
  • Tag / Fusion Protein
    • truncated RTA activation domain (75-190) (C terminal on insert)
    		•
    dSp-Rta 125-190dCas9 (Synthetic) Church Church Lab CRISPR plasmids 2017 (unpublished) Expresses dSp Cas9 fused to truncated RTA activation domain pBR322
  • Tag / Fusion Protein
    • truncated RTA activation domain ( 125-190) (C terminal on insert)
    		•
    dSp-Rta 50-175dCas9 (Synthetic) Church Church Lab CRISPR plasmids 2017 (unpublished) Expresses dSp Cas9 fused to truncated RTA activation domain pBR322
  • Tag / Fusion Protein
    • truncated RTA activation domain (51-175) (C terminal on insert)
    		•
    dSp-Rta 75-175dCas9 (Synthetic) Church Church Lab CRISPR plasmids 2017 (unpublished) Expresses dSp Cas9 fused to truncated RTA activation domain pBR322
  • Tag / Fusion Protein
    • truncated RTA activation domain (75-175) (C terminal on insert)
    		•
    dSp-Rta 100-175dCas9 (Synthetic) Church Church Lab CRISPR plasmids 2017 (unpublished) Expresses dSp Cas9 fused to truncated RTA activation domain pBR322
  • Tag / Fusion Protein
    • truncated RTA activation domain (101-175) (C terminal on insert)
    		•
    dSp-Rta 125-175dCas9 (Synthetic) Church Church Lab CRISPR plasmids 2017 (unpublished) Expresses dSp Cas9 fused to truncated RTA activation domain pBR322
  • Tag / Fusion Protein
    • truncated RTA activation domain (125-175) (C terminal on insert)
    		•
    dSp-VP64-RTA(75-190)dCas9 (Synthetic) Church Church Lab CRISPR plasmids 2017 (unpublished) Expresses dSp Cas9 fused to combination of truncated activation domains pBR322
  • Tag / Fusion Protein
    • VP64-RTA(75-190) (C terminal on insert)
    		•
    dSp-VP64-p65-RTA(75-190)dCas9 (Synthetic) Church Church Lab CRISPR plasmids 2017 (unpublished) Expresses dSp Cas9 fused to combination of truncated activation domains pBR322
  • Tag / Fusion Protein
    • VP64-p65-RTA(75-190) (C terminal on insert)
    		•
    dSp-VP64-p65(100-261)-RTA(75-190)dCas9 (Synthetic) Church Church Lab CRISPR plasmids 2017 (unpublished) Expresses dSp Cas9 fused to combination of truncated activation domains pBR322
  • Tag / Fusion Protein
    • VP64-p65(101-261)-RTA(75-190) (C terminal on insert)
    		•
    dSp-VP64-p65(100-261)-RTA(125-190)dCas9 (Synthetic) Church Church Lab CRISPR plasmids 2017 (unpublished) Expresses dSp Cas9 fused to combination of truncated activation domains pBR322
  • Tag / Fusion Protein
    • VP64-p65(101-261)-RTA(125-190) (C terminal on insert)
    		•
    dSp-VP64-p65(150-261)-RTA(125-190)dCas9 (Synthetic) Church Church Lab CRISPR plasmids 2017 (unpublished) Expresses dSp Cas9 fused to combination of truncated activation domains pBR322
  • Tag / Fusion Protein
    • VP64-P65(151-261)-RTA(125-190) (C terminal on insert)
    		•
    dSp-VP64-RTAdCas9 (Synthetic) Church Church Lab CRISPR plasmids 2017 (unpublished) Expresses dSp Cas9 fused at C term. To VP64 and RTA activation domains pBR322
  • Tag / Fusion Protein
    • VP64-RTA (C terminal on insert)
    		•
    pAAV-dSa-VP64-p65(100-261)-RTA(125-190)dCas9 (Synthetic) Church Church Lab CRISPR plasmids 2017 (unpublished) Expresses dSa Cas9 fused to optimized combination of truncated activation domains pAAV (pUC f1)
  • Tag / Fusion Protein
    • VP64-p65(101-261)-RTA(125-190) (C terminal on insert)
    		•
    pAAV-dSa-VP64dCas9 (Synthetic) Church Church Lab CRISPR plasmids 2017 (unpublished) Expresses dSa Cas9 fused to gold standard VP64 activator pAAV (pUC f1)
  • Tag / Fusion Protein
    • VP64 (C terminal on insert)
    		•
    pAAV-SCP1-dSa-VPR mini.dCas9 (Synthetic) Church Church Lab CRISPR plasmids 2017 (unpublished) Expresses dSa Cas9 fused to optimized combination of truncated activation domains from EFS promoter pAAV (pUC f1)
  • Tag / Fusion Protein
    • VPR mini (C terminal on insert)
    		•
    pAAV-EFS-dSa-VPR mini.dCas9 (Synthetic) Church Church Lab CRISPR plasmids 2017 (unpublished) Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter pAAV (pUC f1)
  • Tag / Fusion Protein
    • VPR mini (C terminal on insert)
    		•
    pAAV-CMV-dSa-VPR mini.-1X snRP1dCas9 (Synthetic) Church Church Lab CRISPR plasmids 2017 (unpublished) Expresses dSa Cas9 fused to optimized combination of truncated activation domains with 17 nt snRP1 poly adenylation signal pAAV (pUC f1)
  • Tag / Fusion Protein
    • VPR mini (C terminal on insert)
    		•
    pAAV-CMV-dSa-VPR mini.-2X snRP1dCas9 (Synthetic) Church Church Lab CRISPR plasmids 2017 (unpublished) Expresses dSa Cas9 fused to optimized combination of truncated activation domains with 34 nt dual snRP1 poly adenylation signal pAAV (pUC f1)
  • Tag / Fusion Protein
    • VPR mini (C terminal on insert)
    		•
    pAAV-CMV-dSa-VPR mini.-syn pAdCas9 (Synthetic) Church Church Lab CRISPR plasmids 2017 (unpublished) Expresses dSa Cas9 fused to optimized combination of truncated activation domains with syn pA (poly adenylation) signal pAAV (pUC f1)
  • Tag / Fusion Protein
    • VPR mini (C terminal on insert)
    		•
    PB-UniSAMUniSAM-mCherry + U6-gRNA2.0EF1amCherry Forrester An all-in-one UniSam vector system for efficient gene activation Scientific Reports Encodes for Cas9-VP64, MS2-p65-HSF1, mCherry and for the gRNA 2.0 Piggy Bac
    pTMt_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1TMt-dCas9(C)-VP64 Fulga Engineering Synthetic Signaling Pathways with Programmable dCas9-Based Chimeric Receptors. Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 10.1016/j.celrep.2017.08.044. Encodes dCas9(C)-VP64 with SAM components fused to transmembrane tether pX856
    pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1VEGFR1-dCas9(C) Fulga Engineering Synthetic Signaling Pathways with Programmable dCas9-Based Chimeric Receptors. Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 10.1016/j.celrep.2017.08.044. Encodes dCas9(C)-VP64 with SAM components fused to VEGFR1 pTMt_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
    pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1BDKRB2-dCas9(N) Fulga Engineering Synthetic Signaling Pathways with Programmable dCas9-Based Chimeric Receptors. Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 10.1016/j.celrep.2017.08.044. Encodes dCas9(C)-VP64 with SAM components fused to BDKRB2 pTMt_TCS(Q’G)_NLSHA- dCas9(C)-VP64_T2A_MCP-P65-HSF1
    pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)_T2A_DHFR-PCP-VP64BDKRB2-dCas9(C)_DHFR-PCP-VP64 Fulga Engineering Synthetic Signaling Pathways with Programmable dCas9-Based Chimeric Receptors. Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 10.1016/j.celrep.2017.08.044. Encodes dCas9(C) with DHFR-PP7-VP64 fused to BDKRB2 pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
    pAVPR2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1AVPR2-dCas9(C)-VP64 Fulga Engineering Synthetic Signaling Pathways with Programmable dCas9-Based Chimeric Receptors. Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 10.1016/j.celrep.2017.08.044. Encodes dCas9(C)-VP64 with SAM components fused to AVPR2 pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
    pCXCR4_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1CXCR4-dCas9(C)-VP64 Fulga Engineering Synthetic Signaling Pathways with Programmable dCas9-Based Chimeric Receptors. Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 10.1016/j.celrep.2017.08.044. Encodes dCas9(C)-VP64 with SAM components fused to CXCR4 pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
    pLPAR1_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1LPAR1-dCas9(C)-VP64 Fulga Engineering Synthetic Signaling Pathways with Programmable dCas9-Based Chimeric Receptors. Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 10.1016/j.celrep.2017.08.044. Encodes dCas9(C)-VP64 with SAM components fused to LPAR1 pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
    IGI-P0492 pHR-dCas9-NLS-VPR-mCherrydCas9-VPR-mCherry (Homo sapiens)CAGmCherry Corn Corn Lab Cas9 plasmids (unpublished) Lentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa. pHR
  • Tag / Fusion Protein
    • NLS-VPR-mCherry (C terminal on insert)
    		•
    PB-SAMMS2-P65-HSF1_T2A_Hygro (Homo sapiens), dCAS9(D10A, N863A)-VP64_T2A_Blast (Synthetic)EF1A, CAGHygromycin, Blasticidin Liu One-Step piggyBac Transposon-Based CRISPR/Cas9 Activation of Multiple Genes. Mol Ther Nucleic Acids. 2017 Sep 15;8:64-76. doi: 10.1016/j.omtn.2017.06.007. Epub 2017 Jun 15. Piggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex. piggybac
    PB-SAM-DestN/AHygromycin, Blasticidin Liu One-Step piggyBac Transposon-Based CRISPR/Cas9 Activation of Multiple Genes. Mol Ther Nucleic Acids. 2017 Sep 15;8:64-76. doi: 10.1016/j.omtn.2017.06.007. Epub 2017 Jun 15. Piggybac transposon DEST vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex. It is used for insertion of multiple U6-sgRNA expression cassettes. PB-SAM
    PB-CAG-DDdCas9VP192-T2A-GFP-IRES-NeoDDdCas9VP192-T2A-GFP-IRES-Neo (Other)CAGNeomycin (select with G418) Otonkoski Human pluripotent reprogramming with CRISPR activators. Nat Commun. 2018 Jul 6;9(1):2643. doi: 10.1038/s41467-018-05067-x. PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection PB-CAG
    PB-CAG-DDdCas9VPH-T2A-GFP-IRES-NeoDDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo (Other)CAGNeomycin (select with G418) Otonkoski Human pluripotent reprogramming with CRISPR activators. Nat Commun. 2018 Jul 6;9(1):2643. doi: 10.1038/s41467-018-05067-x. PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection PB-CAG
    PB-CAG-DDdCas9VPP300-T2A-GFP-IRES-NeoDDdCas9VP192-P300-T2A-GFP-IRES-Neo (Other)CAGNeomycin (select with G418) Otonkoski Human pluripotent reprogramming with CRISPR activators. Nat Commun. 2018 Jul 6;9(1):2643. doi: 10.1038/s41467-018-05067-x. PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection PB-CAG
    PB-CAG-DDdCas9VPPH-T2A-GFP-IRES-NeoDDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo (Other)CAGNeomycin (select with G418) Otonkoski Human pluripotent reprogramming with CRISPR activators. Nat Commun. 2018 Jul 6;9(1):2643. doi: 10.1038/s41467-018-05067-x. PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection PB-CAG
    PB-tight-DDdCas9VP192-T2A-GFP-IRES-NeoDDdCas9VP192-T2A-GFP-IRES-Neo (Other)TRE-tightNeomycin (select with G418) Otonkoski Human pluripotent reprogramming with CRISPR activators. Nat Commun. 2018 Jul 6;9(1):2643. doi: 10.1038/s41467-018-05067-x. PiggyBac transposon system construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection PB-tight
    PB-tight-DDdCas9VPH-T2A-GFP-IRES-NeoDDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo (Other)TRE-tightNeomycin (select with G418) Otonkoski Human pluripotent reprogramming with CRISPR activators. Nat Commun. 2018 Jul 6;9(1):2643. doi: 10.1038/s41467-018-05067-x. PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection PB-tight
    PB-tight-DDdCas9VPP300-T2A-GFP-IRES-NeoDDdCas9VP192-P300-T2A-GFP-IRES-Neo (Other)TRE-tightNeomycin (select with G418) Otonkoski Human pluripotent reprogramming with CRISPR activators. Nat Commun. 2018 Jul 6;9(1):2643. doi: 10.1038/s41467-018-05067-x. PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection PB-tight
    PB-tight-DDdCas9VPPH-T2A-GFP-IRES-NeoDDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo (Other)TRE-tightNeomycin (select with G418) Otonkoski Human pluripotent reprogramming with CRISPR activators. Nat Commun. 2018 Jul 6;9(1):2643. doi: 10.1038/s41467-018-05067-x. PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection PB-tight
    pCXLE-dCas9VPH-T2A-GFP-shP53dCas9VPH-T2A-GFP-shP53 (Other)CAG Otonkoski Human pluripotent reprogramming with CRISPR activators. Nat Commun. 2018 Jul 6;9(1):2643. doi: 10.1038/s41467-018-05067-x. EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette. pCXLE
    pCXLE-dCas9VPP300-T2A-GFP-shP53dCas9VPP300-T2A-GFP-shP53 (Other)CAG Otonkoski Human pluripotent reprogramming with CRISPR activators. Nat Commun. 2018 Jul 6;9(1):2643. doi: 10.1038/s41467-018-05067-x. EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette. pCXLE
    pCXLE-dCas9VPPH-T2A-GFP-shP53dCas9VPPH-T2A-GFP-shP53 (Other)CAG Otonkoski Human pluripotent reprogramming with CRISPR activators. Nat Commun. 2018 Jul 6;9(1):2643. doi: 10.1038/s41467-018-05067-x. EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette. pCXLE
    PB-tight-DDdCas9GFP-IRES-NeoDDdCas9GFP-IRES-Neo (Other)TRE-tightNeomycin (select with G418) Otonkoski Human pluripotent reprogramming with CRISPR activators. Nat Commun. 2018 Jul 6;9(1):2643. doi: 10.1038/s41467-018-05067-x. PiggyBac construct with doxycyline inducible TRE-tight promoter expression of dCas9-GFP followed by IRES-Neomycin selection cassette. PB-tight
    pAW90.dCas9-YY1dCas9-YY1Blasticidin Young YY1 Is a Structural Regulator of Enhancer-Promoter Loops. Cell. 2017 Dec 14;171(7):1573-1588.e28. doi: 10.1016/j.cell.2017.11.008. Epub 2017 Dec 7. Expresses dCas9-YY1 dcas9-vp64
    p-dCas9-VP64-HygroVP64 (Synthetic)CMV promoterHygromycin Beck Beck lab Cas9 plasmids (unpublished) transient expression of dCas9-VP64 fusion protein pMLM3705
    p-dCas9-p300-Hygrop300 (Homo sapiens)CMV promoterHygromycin Beck Beck lab Cas9 plasmids (unpublished) transient expression of dCas9-p300 fusion protein pMLM3705
    p-dCas9-p300-S1396R-S1397R-Hygrodp300 (Homo sapiens)CMV promoterHygromycin Beck Beck lab Cas9 plasmids (unpublished) transient expression of dCas9-dp300 fusion protein pMLM3705
    JG1202: CAG-human dLbCpf1(D832A)-NLS-3xHA-P65dLbCpf1(D832A)-p65 (Other)CAG Joung Inducible and multiplex gene regulation using CRISPR-Cpf1-based transcription factors. Nat Methods. 2017 Oct 30. doi: 10.1038/nmeth.4483. Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to p65 activation domain pCAG-GFP
    JG1211: CAG-human dLbCpf1(D832A)-NLS-3xHA-VPRdLbCpf1(D832A)-VPR (Other)CAG Joung Inducible and multiplex gene regulation using CRISPR-Cpf1-based transcription factors. Nat Methods. 2017 Oct 30. doi: 10.1038/nmeth.4483. Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to VPR activator pCAG-GFP
    CAG-LoxpStopLoxp-NLS-dCas9-HA-NLS-NLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-T2A-EGFP-WPRE-PolyACAG-LSL-dCas9-10xGCN4-P2A-scFv-P65-HSF1-T2A-EGFP-WPRE-PolyA (Other) Yang In vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR-dCas9-activator transgenic mice. Nat Neurosci. 2018 Jan 15. pii: 10.1038/s41593-017-0060-6. doi: 10.1038/s41593-017-0060-6. Encoding an SPH activation system PB
    dSV40-NLS-dCas9-HA-NLS-NLS-10xGCN4dSV40-dCas9-10xGCN4 (Other) Yang In vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR-dCas9-activator transgenic mice. Nat Neurosci. 2018 Jan 15. pii: 10.1038/s41593-017-0060-6. doi: 10.1038/s41593-017-0060-6. Encoding dCas9-10xGCN4 driven by dSV40 promoter Lentivirus
    dxCas(3.7)-VPRdxCas(3.7)-VPR (Synthetic) Liu Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155. Mammalian expression vector for xCas9 3.7 with VP64-p65-Rta (VPR) fused to its C-terminus pCMV
  • Tag / Fusion Protein
    • NLS (N terminal on insert)
    		•
    dxCas(3.6)-VPRdxCas(3.6)-VPR (Synthetic) Liu Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155. Mammalian expression vector for xCas9 3.6 with VP64-p65-Rta (VPR) fused to its C-terminus pCMV
  • Tag / Fusion Protein
    • NLS (N terminal on insert)
    		•
    pPB-VPR-dSpCas9[StaPL(TI)]VPR-dSpCas9[StaPL(TI)] (Homo sapiens)PGKPuromycin Lin StaPLs: versatile genetically encoded modules for engineering drug-inducible proteins. Nat Methods. 2018 Jul;15(7):523-526. doi: 10.1038/s41592-018-0041-z. Epub 2018 Jul 2. Expresses a VPR transcriptional activator fused to the nuclease-deactivated S. pyogenes Cas9, which is governed by an inserted StaPL(TI) module, in mammalian cells. [TI = telaprevir inhibited]. pPB
    dCas9-NS3-NLS/VPRdCas9-NS3-NLS/VPR (Other)CMVHygromycin Ngo Chemogenetic control of gene expression and cell signaling with antiviral drugs. Nat Methods. 2018 Jul;15(7):519-522. doi: 10.1038/s41592-018-0042-y. Epub 2018 Jul 2. Encodes the drug-preservable Cas9-NS3-NLS/VPR, which can be used to activate gene expression when combined with an NS3 inhibitor and appropriately designed sgRNA. pcDNA3
    pKLV2-EF1adCas9VP64T2ABsd-WdCas9VP64-T2A-Blast (Synthetic)Human EF1a promoterBlasticidin Yusa piggyBac-CRISPRa (unpublished) Lentiviral vector expressing Lentiviral vector expressing Lentiviral vector expressing dCas9VP64 under the control of the long human EF1a promoter pKLV2-EF1a-W
    pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESneopAEF1a promoter, dCas9VP64, MS2p65HSF1, IRESneo (Synthetic)Neomycin (select with G418) Yusa piggyBac-CRISPRa (unpublished) Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Neomycin resistant marker pENTR
    pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpAEF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo (Synthetic)Neomycin (select with G418) Yusa piggyBac-CRISPRa (unpublished) Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidine resistant marker pENTR
    pPB-R1R2_EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpAEF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo (Synthetic)Blasticidin Yusa piggyBac-CRISPRa (unpublished) piggyBac transposon vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidin resistant marker pPB_R1R2-EM7neoPheS
    pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpAVP64-dCas9-VP64-T2A-MS2-p65-HSF1 (Homo sapiens)Blasticidin Wright Pooled extracellular receptor-ligand interaction screening using CRISPR activation. Genome Biol. 2018 Nov 26;19(1):205. doi: 10.1186/s13059-018-1581-3. Expresses VP64-dCas9-VP64 and MS2-p65-HSF1 fusion proteins pPB-R1R2-EM7NeoPheS
    pPB-R1R2_EF1ap300dCas9VP64_T2A_MS2p65HSF1-IRESbsdpAp300core-dCas9-VP64-T2A-MS2-p65-HSF1 (Homo sapiens)Blasticidin Wright Pooled extracellular receptor-ligand interaction screening using CRISPR activation. Genome Biol. 2018 Nov 26;19(1):205. doi: 10.1186/s13059-018-1581-3. Expresses p300-dCas9-VP64 and MS2-p65-HSF1 fusion proteins pPB-R1R2-EM7NeoPheS
    pJEP303-pAAV-CMV-dSaCas9-VP64-pAde-catalyzed SaCas9 (Synthetic) Ploski The Development of an AAV-Based CRISPR SaCas9 Genome Editing System That Can Be Delivered to Neurons in vivo and Regulated via Doxycycline and Cre-Recombinase. Front. Mol. Neurosci. (2018) 13 A CMV driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted region AAV
    pJEP304-pAAV-EFS-dSaCas9-VP64-pAde-catalyzed SaCas9 (Synthetic) Ploski The Development of an AAV-Based CRISPR SaCas9 Genome Editing System That Can Be Delivered to Neurons in vivo and Regulated via Doxycycline and Cre-Recombinase. Front. Mol. Neurosci. (2018) 13 A EFS driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted region AAV
    lenti EF1a-FLAG-dCas9-VPRdCas9-VPR (Synthetic)EF1a Day A Neuron-Optimized CRISPR/dCas9 Activation System for Robust and Specific Gene Regulation. eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-0495-18. doi: 10.1523/ENEURO.0495-18.2019. eCollection 2019 Jan-Feb. Lentivirus compatible dCas9-VPR construct driven by the EF1a promoter lentiCRISPR v2
    lenti SYN-FLAG-dCas9-VPRdCas9-VPR (Synthetic)SYN Day A Neuron-Optimized CRISPR/dCas9 Activation System for Robust and Specific Gene Regulation. eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-0495-18. doi: 10.1523/ENEURO.0495-18.2019. eCollection 2019 Jan-Feb. Lentivirus compatible dCas9-VPR construct driven by the human SYN promoter lentiCRISPR v2
    lenti CAG-FLAG-dCas9-VPRdCas9-VPR (Synthetic)CAG Day A Neuron-Optimized CRISPR/dCas9 Activation System for Robust and Specific Gene Regulation. eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-0495-18. doi: 10.1523/ENEURO.0495-18.2019. eCollection 2019 Jan-Feb. Lentivirus compatible dCas9-VPR construct driven by the CAG promoter lentiCRISPR v2
    pT2K-CAGGS-U6-stuffer-dCAs9-VP160-T2A-IRES-mCherrydCas9-VP160mCherry Roussel Mouse medulloblastoma driven by CRISPR activation of cellular Myc. Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1038/s41598-018-24956-1. dCas9-VP160 expression in mammalian cells pT2K-CAGGS-T2TP
    pSH231-EF1-BLST-dCas9-VPRBlastR-P2A-dCas9-VPR (Synthetic)EF1aBlasticidin Monnat New human chromosomal safe harbor sites for genome engineering with CRISPR/Cas9, TAL effector and homing endonucleases bioRxiv 396390 Safe harbor site 231 knock-in vector with BlastR-dCas9-VPR expression cassette Piggybac
    pCMV-sadCas9-VP64dCas9-VP64 (Other)CMVNONE Ahituv CRISPR-mediated activation of a promoter or enhancer rescues obesity caused by haploinsufficiency. Science. 2018 Dec 13. pii: science.aau0629. doi: 10.1126/science.aau0629. An AAV vector expressing S. aureus dCas9 fused to VP64. pAAV-NLS-dSaCas9-NLS-VPR
    pCMV-SpdCas9-VP64VP64 (Other)CMVNONE Ahituv CRISPR-mediated activation of a promoter or enhancer rescues obesity caused by haploinsufficiency. Science. 2018 Dec 13. pii: science.aau0629. doi: 10.1126/science.aau0629. Expresses SpdCas9-Vp64 fusion protein when packaged into AAV pAAV-Ef1a-FAS-EGFP-WPRE-pA
    BICstim-Gag-dCAS9-VPRGAG (FMLV)-dCAS9-VPR (Homo sapiens)hCMV Ohlmann Genome editing in primary cells and in vivo using viral-derived Nanoblades loaded with Cas9-sgRNA ribonucleoproteins. Nat Commun. 2019 Jan 3;10(1):45. doi: 10.1038/s41467-018-07845-z. encodes a GAG-dCAS9-VPR fusion for targeted transcriptional activation. pcDNA3-like
    PB-TetON-dual-SunTag-HygrodCas9-GCN4x10_scFv-VP64 (Mus musculus)Tet-ONHygromycin Navarro The molecular logic of Nanog-induced self-renewal in mouse embryonic stem cells. Nat Commun. 2019 Mar 7;10(1):1109. doi: 10.1038/s41467-019-09041-z. Endogenous gene activation with the SunTag CRISPR activation system PiggyBac
    FU_tetO_dCas9-VP192-T2A-EGFPdCas9-VP192-T2A-EGFP (Other)EGFP Otonkoski Otonkoski plasmids 2018 (unpublished) Lentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP. FU_tetOp
    IA304: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-VPRSa-dCas9/VPR (open) (Synthetic) Newgard Creation of versatile cloning platforms for transgene expression and dCas9-based epigenome editing. Nucleic Acids Res. 2018 Dec 27. pii: 5264291. doi: 10.1093/nar/gky1286. pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the VPR transcriptional activator for 3 or 4-component MultiSite Gateway Pro assembly. pDONR221 P4r-P3r
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)
    		•
    KN801: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-VPRx-dCas9(3.7)/VPR (open) (Synthetic) Newgard Creation of versatile cloning platforms for transgene expression and dCas9-based epigenome editing. Nucleic Acids Res. 2018 Dec 27. pii: 5264291. doi: 10.1093/nar/gky1286. pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the VPR transcriptional activator for 3 or 4-component MultiSite Gateway Pro assembly. pDONR221 P4r-P3r
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)
    		•
    KL001: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-VPR2x NLS/VPR (open) (Synthetic) Newgard Creation of versatile cloning platforms for transgene expression and dCas9-based epigenome editing. Nucleic Acids Res. 2018 Dec 27. pii: 5264291. doi: 10.1093/nar/gky1286. pMAGIC R4-R3 entry plasmid, contains 2x NLS/VPR scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites pDONR221 P4r-P3r
    pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPREdCas9 fused to SunTag(24x)SFFV Brangwynne Liquid Nuclear Condensates Mechanically Sense and Restructure the Genome. Cell. 2018 Nov 29;175(6):1481-1491.e13. doi: 10.1016/j.cell.2018.10.057. Expresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP. pHR
    ARB367PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::VPR-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA (Synthetic) El-Samad A Toolkit for Rapid Modular Construction of Biological Circuits in Mammalian Cells. ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 10.1021/acssynbio.9b00322. Epub 2019 Nov 5. Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to VPR domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector MTK0_043
    PB_tre_dCas9_VP160dCas9 fusion with VP160, Hygromycin resistanceTRE, EF1 alphaHygromycin Calabrese A piggyBac-based toolkit for inducible genome editing in mammalian cells. RNA. 2019 May 17. pii: rna.068932.118. doi: 10.1261/rna.068932.118. PiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusion piggyBac cargo vector
    pAN1646p7x_tet-Venus-tADH1-pGal1-dCas9-VP64-Linker-mScarlet-Linker-degronSwitch_A_t12-tPGK1-pZ3-key_A_e18-mTagBFP2-NLS(SV40)-tSSA1 (Synthetic)URA3 El-Samad De novo design of bioactive protein switches. Nature. 2019 Jul 24. pii: 10.1038/s41586-019-1432-8. doi: 10.1038/s41586-019-1432-8. pTet expression of Venus, Dual inducible expression of dCas9-VP64-mScarlet-degronSwitchA and KeyA-BFP-NLS Custom MoClo Backbone
    VPR_dCas9VPR-dCas9-2A-ZsGreen-DR (Synthetic)CMV Pedersen Awakening dormant glycosyltransferases in CHO cells with CRISPRa. Biotechnol Bioeng. 2020 Feb;117(2):593-598. doi: 10.1002/bit.27199. Epub 2019 Nov 12. Express CHO codon-optimized dCas9 fused to VPR for CRISPR activation. 2A linked ZsGreen1 marker pJ204
    3XFLAG-VP64-SadCas9-NLS-VP642xVP64-SadCas9 (Other)CMV Cohn A mutation-independent approach for muscular dystrophy via upregulation of a modifier gene. Nature. 2019 Aug;572(7767):125-130. doi: 10.1038/s41586-019-1430-x. Epub 2019 Jul 24. 3xFLAG VP64 SadCas9 for single guide cloning pX601
    pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPREdSpCas9-VPR (Other)Puromycin Sanjana High-Throughput Screens of PAM-Flexible Cas9 Variants for Gene Knockout and Transcriptional Modulation. Cell Rep. 2020 Mar 3;30(9):2859-2868.e5. doi: 10.1016/j.celrep.2020.02.010. Expresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette. lentiCRISPRv2
    pCC_06 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9NG-NLS-VPR-2A-Puro-WPREdSpCas9-NG-VPR (Other)Puromycin Sanjana High-Throughput Screens of PAM-Flexible Cas9 Variants for Gene Knockout and Transcriptional Modulation. Cell Rep. 2020 Mar 3;30(9):2859-2868.e5. doi: 10.1016/j.celrep.2020.02.010. Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette. lentiCRISPRv2
    pCC_07 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9-NLS-VPR-2A-Puro-WPREdxCas9 3.7-VPR (Other)Puromycin Sanjana High-Throughput Screens of PAM-Flexible Cas9 Variants for Gene Knockout and Transcriptional Modulation. Cell Rep. 2020 Mar 3;30(9):2859-2868.e5. doi: 10.1016/j.celrep.2020.02.010. Expresses human codon-optimized inactive xCas9 3.7 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette. lentiCRISPRv2
    pCC_08 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9NG-NLS-VPR-2A-Puro-WPREdxCas9-NG-VPR (Other)Puromycin Sanjana High-Throughput Screens of PAM-Flexible Cas9 Variants for Gene Knockout and Transcriptional Modulation. Cell Rep. 2020 Mar 3;30(9):2859-2868.e5. doi: 10.1016/j.celrep.2020.02.010. Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette. lentiCRISPRv2
    pLentiV2-dCas9-VP64Puromycin Ulitsky pLentiV2-dCas9-VP64 (unpublished) Expression of gRNA and dCas9-VP64 from the same plasmid for CRISPRa entiSAM v2 (Puro) addgene#92062
    pCAG-scFvGCN4sfGFP-VP64-GB1scFvGCN4sfGFP-VP64-GB1CAGNeomycin (select with G418) Hatada Synergistic Upregulation of Target Genes by TET1 and VP64 in the dCas9-SunTag Platform. Int J Mol Sci. 2020 Feb 25;21(5). pii: ijms21051574. doi: 10.3390/ijms21051574. Express scFvGCN4-sfGFP-VP64 fusion protein for dCas9-SunTag mediated targeted gene activation pCAG

    Bacteria

    Plasmid Gene/Insert Promoter PI Publication Hidden Extra Search Info
    pWJ66tracrRNA (Other), dcas9-w (Other), CRISPR array Marraffini Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic Acids Res. 2013 Jun 12. Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9) pACYC184
    pWJ68tracrRNA (Other), w-dcas9 (Other), CRISPR array Marraffini Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic Acids Res. 2013 Jun 12. Same as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9) pACYC184
    pET-dCas9-VP64-6xHisdCas9-VP64 (Other)T7 Liu Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo. Nat Biotechnol. 2015 Jan;33(1):73-80. doi: 10.1038/nbt.3081. Epub 2014 Oct 30. Expression of dCas9-VP64-6xHis in bacterial cells pET29
    pET-deSpCas9-VP64-6xHisdead/inactive eSpCas9-NLS-3xFLAG-VP64 (Other)T7 Welker Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage. Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8. Expression of dead/inactive increased fidelity eSpCas9 (1.1)-VP64-6xHis in bacterial cells pET29
    pET-dSpCas9-HF1-VP64-6xHisdead/inactive SpCas9-HF1-NLS-3xFLAG-VP64 (Other)T7 Welker Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage. Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8. Expression of dead/inactive increased fidelity SpCas9-HF1-VP64-6xHis in bacterial cells pET29
    pET-dHeFSpCas9-VP64-6xHisdead/inactive HeFSpCas9-NLS-3xFLAG-VP64 (Other)T7 Welker Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage. Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8. Expression of dead/inactive increased fidelity HeFSpCas9-VP64-6xHis in bacterial cells pET29
    pCD185dCas9 and MCP-SoxS_R93A (Other) Zalatan Synthetic CRISPR-Cas gene activators for transcriptional reprogramming in bacteria. Nat Commun. 2018 Jun 27;9(1):2489. doi: 10.1038/s41467-018-04901-6. dCas9, MCP-SoxS_R93A p15A vector
    pCD227dCas9 and MCP-SoxS_R93A (Other) Zalatan Synthetic CRISPR-Cas gene activators for transcriptional reprogramming in bacteria. Nat Commun. 2018 Jun 27;9(1):2489. doi: 10.1038/s41467-018-04901-6. AraC-pBAD-dCas9, MCP-SoxS_R93A p15A vector
    pJF093dCas9 and MCP-SoxS_R93A (Other) Zalatan Synthetic CRISPR-Cas gene activators for transcriptional reprogramming in bacteria. Nat Commun. 2018 Jun 27;9(1):2489. doi: 10.1038/s41467-018-04901-6. Sp.pCas9-dCas9, pTet-MCP-SoxS_R93A p15A vector
    pJF104BdCas9 and MCP-SoxS_R93A (Other) Zalatan Synthetic CRISPR-Cas gene activators for transcriptional reprogramming in bacteria. Nat Commun. 2018 Jun 27;9(1):2489. doi: 10.1038/s41467-018-04901-6. pTet-dCas9, pTet-MCP-SoxS_R93A p15A vector
    pdCas9-omegadCas9-omega (Other)Tet promoter Chatterjee Multiplexed deactivated CRISPR-Cas9 gene expression perturbations deter bacterial adaptation by inducing negative epistasis. Commun Biol. 2018 Sep 3;1:129. doi: 10.1038/s42003-018-0135-2. eCollection 2018. Addgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAP p15a
    pFD116Ptet (Other), dcas9 (Other), PpflB sgRNA BsaI (Synthetic) Bikard Gene silencing with CRISPRi in bacteria and optimization of dCas9 expression levels. Methods. 2019 Aug 1. pii: S1046-2023(18)30497-3. doi: 10.1016/j.ymeth.2019.07.024. pFD116 carries dcas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector pLZ12
    pLY9dcas9 (Other), tetR (Other), pspFΔHTH::λN22plus (Synthetic), sfgfp (Synthetic)Ptet, Ptet, Anderson promoter: J23106, PpspA-2G6 Wang Engineered CRISPRa enables programmable eukaryote-like gene activation in bacteria Nature Communications 10, August 2019 A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV). pSB4A3mut
    pLY53dcas9 (Other), tetR (Other), pspFΔHTH::λN22plus (Synthetic), sfgfp (Synthetic)Ptet, Ptet, Anderson promoter: J23106, PpspA-LEA1B1 Wang Engineered CRISPRa enables programmable eukaryote-like gene activation in bacteria Nature Communications 10, August 2019 A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA1B1 with sfgfp::ASV). pSB4A3mut
    pLY54dcas9 (Other), tetR (Other), pspFΔHTH::λN22plus (Synthetic), sfgfp (Synthetic)Ptet, Ptet, Anderson promoter: J23106, PpspA-LEA2B2 Wang Engineered CRISPRa enables programmable eukaryote-like gene activation in bacteria Nature Communications 10, August 2019 A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA2B2 with sfgfp::ASV). pSB4A3mut
    pLY55dcas9 (Other), tetR (Other), pspFΔHTH::λN22plus (Synthetic), sfgfp (Synthetic)Ptet, Ptet, Anderson promoter: J23106, PpspA-LEA3B3 Wang Engineered CRISPRa enables programmable eukaryote-like gene activation in bacteria Nature Communications 10, August 2019 A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV). pSB4A3mut
    pLY56dcas9 (Other), tetR (Other), pspFΔHTH::λN22plus (Synthetic), sfgfp (Synthetic)Ptet, Ptet, Anderson promoter: J23106, PpspA-LEA4B4 Wang Engineered CRISPRa enables programmable eukaryote-like gene activation in bacteria Nature Communications 10, August 2019 A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA4B4 with sfgfp::ASV). pSB4A3mut
    pLY57dcas9 (Other), tetR (Other), pspFΔHTH::λN22plus (Synthetic), sfgfp (Synthetic)Ptet, Ptet, Anderson promoter: J23106, PpspA-LEA5B5 Wang Engineered CRISPRa enables programmable eukaryote-like gene activation in bacteria Nature Communications 10, August 2019 A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA5B5 with sfgfp::ASV). pSB4A3mut
    pLY59dcas9 (Synthetic), tetR (Other), sfgfp (Synthetic), pspFΔHTH::MCP (Other)Ptet, Ptet, PpspA-2G6, Anderson promoter: J23106 Wang Engineered CRISPRa enables programmable eukaryote-like gene activation in bacteria Nature Communications 10, August 2019 A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::MCP controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV). pSB4A3mut
    pLY61dxcas9 (3.7) (Synthetic), tetR (Other), pspFΔHTH::λN22plus (Synthetic), sfgfp (Synthetic)Ptet, Ptet, Anderson promoter: J23106, PpspA-LEA1B1 Wang Engineered CRISPRa enables programmable eukaryote-like gene activation in bacteria Nature Communications 10, August 2019 A CRISPR activation device with the necessary genes (dxcas9 3.7 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA1B1 with sfgfp::ASV). pSB4A3mut
    pLY70dxcas9 (3.7) (Other), tetR (Other), rhaS (Other), pspFΔHTH::λN22plus (Synthetic), sfgfp (Synthetic)Ptet, Ptet, Pcon, PrhaB, PpspA-LEA3B3 Wang Engineered CRISPRa enables programmable eukaryote-like gene activation in bacteria Nature Communications 10, August 2019 A CRISPR activation device with the necessary genes (dxcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter PrhaB), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV). pSB4A3mut
    pLY152dcas9 (Other), tetR (Other), sfgfp (Synthetic)Ptet, Ptet, PpspA-2G6 Wang Engineered CRISPRa enables programmable eukaryote-like gene activation in bacteria Nature Communications 10, August 2019 dcas9 generator and reporter circuit (PpspA-2G6 with sfgfp::ASV) pSB4A3mut

    Drosophila

    Plasmid Gene/Insert Promoter PI Publication
    pAWG-dCas9-VPRSP-dCas9-VPR (Drosophila melanogaster)act5c Church Highly efficient Cas9-mediated transcriptional programming. Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312.
    pWalium20-10XUAS-3XFLAG-dCas9-VPRdCas9-VPR (Homo sapiens)10XUAS Perrimon In Vivo Transcriptional Activation Using CRISPR/Cas9 in Drosophila. Genetics. 2015 Oct;201(2):433-42. doi: 10.1534/genetics.115.181065. Epub 2015 Aug 5.
    pAct:dCas9-VPRdCas9-VPRpActin (Drosophila) Perrimon Comparison of Cas9 activators in multiple species. Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
    pAct:dCas9-GCN4dCas9-10XGCN4pActin (Drosophila) Perrimon Comparison of Cas9 activators in multiple species. Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
    pAct:dCas9-VP64dCas9-VP64pActin (Drosophila) Perrimon Comparison of Cas9 activators in multiple species. Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.

    Plant

    Plasmid Gene/Insert Promoter Selectable Marker PI Publication Hidden Extra Search Info
    pBUN6A11dCas9-VP64 (Synthetic), gRNA scaffold (Synthetic)Ubi1p, OsU3pBar Chen A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance pGreen-like binary vector
    pHSN6A01dCas9-VP64 (Synthetic), gRNA scaffold (Synthetic)2×35Sp, AtU6-26pHygromycin Chen A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance pGreen-like binary vector
    pYPQ152pco-dCas9-VP64 (Plant codon-optimized) (Other) Qi A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation. Plant Physiol. 2015 Aug 21. pii: pp.00636.2015. Gateway entry vector with pco-dCas9-VP64 pYPQ185-linker1
    pYPQ173pco-dCas9-VP64-T2A-MS2-VP64 (Synthetic) Qi Robust transcriptional activation in plants using multiplexed CRISPR-Act2.0 and mTALE-Act systems. Mol Plant. 2017 Nov 29. pii: S1674-2052(17)30344-1. doi: 10.1016/j.molp.2017.11.010. Express CRISPR-Act2.0 system which contains pco-dCas9-VP64 fusion protein and MS2-VP64 fusion protein linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence unknown
    pEG302 5aa SunTag VP64 nogNOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCSHygromycin Jacobsen Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems. Nat Commun. 2019 Feb 13;10(1):729. doi: 10.1038/s41467-019-08736-7. CRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide) pEG302
    pEG302 22aa SunTag NtDRMcd nogNOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCSHygromycin Jacobsen Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems. Nat Commun. 2019 Feb 13;10(1):729. doi: 10.1038/s41467-019-08736-7. CRISPR-Cas9 SunTag system to target NtDRMcd to specific loci of interest (nog=no guide) pEG302
    pEG302 22aa SunTag NtDRMcd (noNLS) nogNOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCSHygromycin Jacobsen Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems. Nat Commun. 2019 Feb 13;10(1):729. doi: 10.1038/s41467-019-08736-7. CRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to specific loci of interest (nog=no guide) pEG302
    pEG302 22aa SunTag VP64 nogNOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCSHygromycin Jacobsen Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems. Nat Commun. 2019 Feb 13;10(1):729. doi: 10.1038/s41467-019-08736-7. CRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide) pEG302
    PV225dCas9-AT-CBF1 (Other) Young Repurposing of Type I-E CRISPR-Cascade for gene activation in plants Nature Communications Biology dCas9 plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea mays PHP17720
  • Tag / Fusion Protein
    • AT-CBF1 (C terminal on insert)
    • CBF1 AT4G25490, ATCBF1, C-repeat/DRE binding factor 1, DRE BINDING PROTEIN 1B, DREB1B, T30C3.11, TRANSCRIPTIONAL ACTIVATOR CBF1
    pYPQ239A (dFnCas12a)-TVdFnCas12a-TV (Other) Qi CRISPR–Cas12b enables efficient plant genome engineering Nature Plants (2020) CRISPRa Gateway entry clone for catalytically dead FnCas12a fused with TV activation system pYPQ167
    pYPQ292 (AaCas12b)-D570A-TVAaCas12b (D570A)-TV (Other) Qi CRISPR–Cas12b enables efficient plant genome engineering Nature Plants (2020) CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system pYPQ167
    pYPQ292 (AaCas12b)-D570A-TV-MS2-TVAaCas12b (D570A)-TV-T2A-MCP-TV (Other) Qi CRISPR–Cas12b enables efficient plant genome engineering Nature Plants (2020) CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-TV fusion protein pYPQ167
    pYPQ292 (AaCas12b)-D570A-TV-MS2-VPRAaCas12b (D570A)-TV-T2A-MCP-VPR (Other) Qi CRISPR–Cas12b enables efficient plant genome engineering Nature Plants (2020) CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-VPR fusion protein pYPQ167

    Yeast

    Plasmid Gene/Insert Promoter Selectable Marker PI Publication Hidden Extra Search Info
    pTPGI_dCas9_VP64dCas9_VP64 (codon-optimized for expression in S. cerevisiae) (Saccharomyces cerevisiae)pTPGI (galactose+aTc inducible)TRP1 Lu Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11. encodes yeast-optimized dCas9_VP64 synthetic transcription factor pTPGI (pRS304)
    pJZC519dCas9-VP64 (Other)pTdh3LEU2 Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Yeast dCas9-VP64 expression plasmid pNH605
    pJZC620MCP-VP64, PCP-VP64, dCas9pAdh, pAdh, pTdh3LEU2 Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Expresses dCas9, MCP-VP64, and PCP-VP64 in Yeast cells pNH605
    pJZC638MCP-VP64, dCas9 (Other)pAdh, pGal10LEU2 Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Expresses MCP-VP64 and dCas9 in Yeast cells pNH605
    pJZC548sgRNA + 1x PP7SNR52URA3 Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA with 1x PP7 for yeast cells pRS416
    pJZC595MCP-VP64, dCas9pAdh, Tdh3LEU2 Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Expresses MCP-VP64 and dCas9 in Yeast cells pNH605
    pAG414GPD-dCas9-VPRdCas9-VPR (Saccharomyces cerevisiae)GPDTRP1 Church Highly efficient Cas9-mediated transcriptional programming. Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312. pAG414 series plasmid with GPD promoter driving expression of dCas9-VPR; cerevisiae vector pAG414GPD
    pRS416-Gal4-dCas9-VP64Gal4-dCas9-VP64 (Saccharomyces cerevisiae)Tef1URA3 Davis Dissecting the Genetic Basis of a Complex cis-Regulatory Adaptation. PLoS Genet. 2015 Dec 29;11(12):e1005751. doi: 10.1371/journal.pgen.1005751. eCollection 2015 Dec. This plasmid contains a Cas9 Activator for yeast. The activator is about 1.2-1.8 X more potent than just dCas9-VP64 fusion in yeast. It is on a single copy CEN/ARS plasmid with Ura marker, pRS416. pRS416
    dCas9v2dCas9-VPR (Saccharomyces cerevisiae)URA3 Nielsen Multiplexed CRISPR/Cas9 Genome Editing and Gene Regulation using Csy4 in Saccharomyces cerevisiae. ACS Synth Biol. 2017 Nov 21. doi: 10.1021/acssynbio.7b00259. TetR inducible dCas9-VPR plasmid with pRPR-NotI-tRPR1 Csy4-ready site pDTU-113
    pCRISPRa_VPR_ylCodon optimized dCas9-VPR (Synthetic), sgRNA expression cassette (Synthetic)UAS1B8-TEF(136), SCR1'-tRNALEU2 Wheeldon Multiplexed CRISPR activation of cryptic sugar metabolism enables Yarrowia lipolytica growth on cellobiose. Biotechnol J. 2018 May 5:e1700584. doi: 10.1002/biot.201700584. CRISPR-dCas9-VPR vector for Yarrowia lipolytica, expressing dCas9-VPR and AvrII site for sgRNA insertion pUC19


    Do you have suggestions for other plasmids that should be added to this list?

    Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource!