CRISPR Plasmids: Activate
Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest. If the plasmid that you choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator to your specific locus.
Plasmids are available for expression in mammalian systems, bacteria, Drosophila, plants, and yeast.
Mammalian
| Plasmid | Gene/Insert | Promoter | Selectable Marker | PI | Publication |
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Bacteria
| Plasmid | Gene/Insert | Promoter | PI | Publication |
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Drosophila
| Plasmid | Gene/Insert | Promoter | PI | Publication |
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Plant
| Plasmid | Gene/Insert | Promoter | Selectable Marker | PI | Publication |
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Yeast
| Plasmid | Gene/Insert | Promoter | Selectable Marker | PI | Publication |
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Do you have suggestions for other plasmids that should be added to this list?
Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource!