CRISPR Plasmids: Interfere
Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB, can knock down gene expression by interfering with transcription. Design your gRNA to target your gene of interest’s promoter/enhancer or the beginning of the coding sequence. If the plasmid you’re using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-repressor to your specific locus.
Plasmids are available for expression in mammalian systems, bacteria, plants, and yeast.
Mammalian
| Plasmid | Gene/Insert | Promoter | Selectable Marker | PI | Publication |
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Bacteria
| Plasmid | Gene/Insert | Promoter | PI | Publication |
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Plant
| Plasmid | Gene/Insert | Promoter | Selectable Marker | PI | Publication |
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Yeast
| Plasmid | Gene/Insert | Promoter | Selectable Marker | PI | Publication |
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