| pdCas9-humanized | dead Cas9 with 3X NLS (Homo sapiens) | MSCV LTR promoter | Puromycin |
Qi
| Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression.
Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022.
|
Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown
pMSCVpuro
|
| pdCas9::BFP-humanized | dCas9 fused to BFP (Homo sapiens) | MSCV LTR promoter | Puromycin |
Qi
| Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression.
Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022.
|
Expression of a catalytically inactive, human codon-optimized Cas9-BFP fusion under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown
pMSCVpuro
|
| pHR-SFFV-dCas9-BFP | dCas9-BFP fusion (Homo sapiens) | SFFV | |
Weissman
| CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes.
Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044.
|
Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS and tagBFP
pHR
|
| pHR-SFFV-dCas9-BFP-KRAB | dCas9-BFP-KRAB fusion (Homo sapiens) | SFFV | |
Weissman
| CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes.
Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044.
|
Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domain
pHR
|
| pcDNA-dCas9 | dCas9 (Other) | CMV | |
Gersbach
| RNA-guided gene activation by CRISPR-Cas9-based transcription factors.
Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2600.
|
Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells
pcDNA3.1
|
| Cas9m2 | Cas9m2 (Other) | | |
Church
| CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering.
Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675.
|
Cas9 D10A+H840A
pcDNA3.3_TOPO
|
| Cas9m3 | Cas9m3 (Other) | | |
Church
| CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering.
Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675.
|
Cas9 D10A+D839A+H840A
pcDNA3.3_TOPO
|
| Cas9m4 | Cas9m4 (Other) | | |
Church
| CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering.
Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675.
|
Cas9 D10A+D839A+H840A+N863A
pcDNA3.3_TOPO
|
| pHAGE TRE dCas9 | dCas9 (Other) | TRE | Neomycin (select with G418) |
Wolfe
| Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells.
Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341.
|
Tet-regulatable dCas9 lentiviral expression vector
pHAGE
|
| pHAGE TRE dCas9-KRAB | dCas9 (Other) | TRE | Neomycin (select with G418) |
Wolfe
| Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells.
Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341.
|
Tet-regulatable dCas9-KRAB lentiviral expression vector
pHAGE
|
| pHAGE EF1α dCas9-KRAB | dCas9 (Other) | EF1alpha | Puromycin |
Wolfe
| Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells.
Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341.
|
Constitutive dCas9-KRAB lentiviral expression vector
pHAGE
|
| pSLQ1658-dCas9-EGFP | dCas9 fuse to EGFP (Homo sapiens) | MSCV LTR promoter | Puromycin |
Qi
| Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System.
Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001.
|
Template for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging (the recipient vector can be TetON 3G promoter system)
pMSCVpuro
|
| pLV hUbC-dCas9-T2A-GFP | humanized dead Cas9 T2A GFP (Other) | hUbC | Zeo marker is outside the LTRs and will not be packaged into virus. |
Gersbach
| Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector.
Nucleic Acids Res. 2014 Aug 13. pii: gku749.
|
Co-expresses human optimized S. pyogenes dCas9 and GFP
FUGW
|
| pcDNA3.1-CibN-dCas9 | CibN-dCas9 (Arabidopsis thaliana) | CMV | Neomycin (select with G418) |
Gersbach
| A light-inducible CRISPR-Cas9 system for control of endogenous gene activation.
Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753.
|
Expresses CibN-dCas9 in mammalian cells
pcDNA3.1
|
| pcDNA3.1-dCas9-CibN | dCas9-CibN (Arabidopsis thaliana) | CMV | Neomycin (select with G418) |
Gersbach
| A light-inducible CRISPR-Cas9 system for control of endogenous gene activation.
Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753.
|
Expresses dCas9-CibN in mammalian cells
pcDNA3.1
|
| pcDNA3.1-CibN-dCas9-CibN | dCas9-CibN (Arabidopsis thaliana) | CMV | Neomycin (select with G418) |
Gersbach
| A light-inducible CRISPR-Cas9 system for control of endogenous gene activation.
Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753.
|
Expresses CibN-dCas9-CibN in mammalian cells
pcDNA3.1
|
| pHR-SFFV-KRAB-dCas9-P2A-mCherry | KRAB-dCas9-P2A-mCherry fusion (Homo sapiens) | SFFV | mCherry |
Weissman
| Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation.
Cell. 2014 Oct 23;159(3):647-61. doi: 10.1016/j.cell.2014.09.029. Epub 2014 Oct 9.
|
2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9 fusion protein and mCherry
pHR
|
| pcDNA-dCas9-HA | S.pyogenes dCas9 with c-terminal HA tag (Synthetic) | CMV | |
Gersbach
| Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers.
Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6.
|
encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter
pcDNA3.1
|
| pX330A_dCas9-1x2 | humanized S. pyogenes dCas9 (Other) | CBh | |
Yamamoto
| Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes.
BMC Biotechnol. 2015 May 22;15(1):33.
|
Expresses dCas9 and gRNA
pUC ori vector
|
| pX330A_dCas9-1x3 | humanized S. pyogenes dCas9 (Other) | CBh | |
Yamamoto
| Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes.
BMC Biotechnol. 2015 May 22;15(1):33.
|
Expresses dCas9 and gRNA
pUC ori vector
|
| pX330A_dCas9-1x5 | humanized S. pyogenes dCas9 (Other) | CBh | |
Yamamoto
| Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes.
BMC Biotechnol. 2015 May 22;15(1):33.
|
Expresses dCas9 and gRNA
pUC ori vector
|
| pX330A_dCas9-1x6 | humanized S. pyogenes dCas9 (Other) | CBh | |
Yamamoto
| Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes.
BMC Biotechnol. 2015 May 22;15(1):33.
|
Expresses dCas9 and gRNA
pUC ori vector
|
| pEF1a-CRY2PHR-SID4X | SID4X-CYR2PHR (Synthetic) | EF1alpha | |
Kong
| Aspirin cooperates with p300 to activate the acetylation of H3K9 and promote FasL-mediated apoptosis of cancer stem-like cells in colorectal cancer
Theranostics
|
AAV expression of CRYS2PHR-SID4X
pAAV
|
| px330-CIB1-dCas9 | | U6 and CMV | |
Kong
| Aspirin cooperates with p300 to activate the acetylation of H3K9 and promote FasL-mediated apoptosis of cancer stem-like cells in colorectal cancer
Theranostics
|
Mammalian gRNA expression vector also expressing CIB1-dCas9
px330
|
| pCCL-PGK-SPdCas9-BFP-DNMT1 | dCas9-BFP-DNMT1, catalytic domain | | |
Luo
| Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases.
Gigascience. 2018 Mar 1;7(3):1-19. doi: 10.1093/gigascience/giy011.
|
dCas9 fused to BFP and the human DNMT1 catalytic domain
3rd Generation lentiviral vector
|
| pCCL-PGK-SPdCas9-BFP-DNMT3A | dSpCas9-BFP-DNMT3A, catalytic domain of DNMT3A (Synthetic) | | |
Luo
| Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases.
Gigascience. 2018 Mar 1;7(3):1-19. doi: 10.1093/gigascience/giy011.
|
dCas9 fused to BFP and the human DNMT3A catalytic domain
3rd Generation lentiviral vector
|
| pCCL-PGK-SPdCas9-BFP-DNMT3B | dSpCas9-BFP-DNMT3B, catalytic domain of DNMT3B (Synthetic) | | |
Luo
| Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases.
Gigascience. 2018 Mar 1;7(3):1-19. doi: 10.1093/gigascience/giy011.
|
dCas9 fused to BFP and the human DNMT3B catalytic domain
3rd Generation lentiviral vector
|
| pCCL-PGK-SPdCas9-BFP-EGFP | dSpCas9-BFP-EGFP (Synthetic) | | |
Luo
| Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases.
Gigascience. 2018 Mar 1;7(3):1-19. doi: 10.1093/gigascience/giy011.
|
dCas9 fused to BFP and EGFP
3rd Generation lentiviral vector
|
| pEF_dCas9 | dCas9 (Other) | human EF1[alpha] | |
Rinn
| Multiplexable, locus-specific targeting of long RNAs with CRISPR-Display.
Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1.
|
Transient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter.
pNEB193
Tag
/ Fusion Protein- 3xFLAG (C terminal on insert)
|
| pCCL-PGK-SPdCas9-BFP-DNMT3A(E752A) | dSpCas9-BFP-DNMT3A(E752A), DNMT3A catalytic domain with inactivation mutation E752A (Synthetic) | | |
Luo
| Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases.
Gigascience. 2018 Mar 1;7(3):1-19. doi: 10.1093/gigascience/giy011.
|
dCas9 fused to BFP and the human DNMT3A catalytically inactive domain
3rd Generation lentiviral vector
|
| pCCL-PGK-SPdCas9-BFP-DNMT3B(E697A) | dSpCas9-BFP-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A (Other) | | |
Luo
| Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases.
Gigascience. 2018 Mar 1;7(3):1-19. doi: 10.1093/gigascience/giy011.
|
dCas9 fused to BFP and the human DNMT3B catalytically inactive domain
3rd Generation lentiviral vector
|
| pLenti-EF1a-SPdCas9-EGFP-2A-Blast | dSpCas9-EGFP (Synthetic) | | |
Luo
| Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases.
Gigascience. 2018 Mar 1;7(3):1-19. doi: 10.1093/gigascience/giy011.
|
dCas9 fused to EGFP
3rd generation lentiviral vector
|
| pLenti-EF1a-SPdCas9-DNMT3A-2A-Blast | dSpCas9-DNMT3A catalytic domain (Synthetic) | | |
Luo
| Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases.
Gigascience. 2018 Mar 1;7(3):1-19. doi: 10.1093/gigascience/giy011.
|
dCas9 fused to human DNMT3A catalytic domain
3rd generation lentiviral vector
|
| pLenti-EF1a-SPdCas9-DNMT3B-2A-Blast | dSpCas9-DNMT3B catalytic domain (Synthetic) | | |
Luo
| Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases.
Gigascience. 2018 Mar 1;7(3):1-19. doi: 10.1093/gigascience/giy011.
|
dCas9 fused to human DNMT3B catalytic domain
3rd generation lentiviral vector
|
| pLenti-EF1a-SPdCas9-DNMT3A(E752A)-2A-Blast | dSpCas9-DNMT3A(E752A) | | |
Luo
| Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases.
Gigascience. 2018 Mar 1;7(3):1-19. doi: 10.1093/gigascience/giy011.
|
dCas9 fused to the human DNMT3A catalytically inactive domain
3rd generation lentiviral vector
|
| pLenti-EF1a-SPdCas9-DNMT3B(E697A)-2A-Blast | dSpCas9-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A (Other) | | |
Luo
| Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases.
Gigascience. 2018 Mar 1;7(3):1-19. doi: 10.1093/gigascience/giy011.
|
dCas9 fused to the human DNMT3B catalytically inactive domain
3rd Generation lentiviral vector
|
| pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro | humanized dCas9-KRAB T2A Puro (Other), sgRNA | | Puromycin |
Gersbach
| Highly specific epigenome editing by CRISPR-Cas9 repressors for silencing of distal regulatory elements.
Nat Methods. 2015 Dec;12(12):1143-9. doi: 10.1038/nmeth.3630. Epub 2015 Oct 26.
|
Express sgRNA and dCas9-KRAB from lentiviral vector
FUGW
|
| pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP | humanized dCas9-KRAB T2A GFP (Other), sgRNA | | |
Gersbach
| Highly specific epigenome editing by CRISPR-Cas9 repressors for silencing of distal regulatory elements.
Nat Methods. 2015 Dec;12(12):1143-9. doi: 10.1038/nmeth.3630. Epub 2015 Oct 26.
|
Express sgRNA and dCas9-KRAB from 3rd generation lentiviral vector
FUGW
|
| pCR1003 | T7pr_10xHis-MBP-TEV-SPydCas9 (D10A, H840A) | | |
Corn
| Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA.
Nat Biotechnol. 2016 Jan 20. doi: 10.1038/nbt.3481.
|
Express Streptococcus pyogenes dCas9 (D10A, H840A)
SPynCas9
|
| pCR1056 | T7pr_6xHis-MBP-TEV-SPydCas9 (D10A,H840A)-2xNLS | | |
Corn
| Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA.
Nat Biotechnol. 2016 Jan 20. doi: 10.1038/nbt.3481.
|
Express Streptococcus pyogenes dCas9 (D10A, H840A) carrying two C-terminal SV40 NLS
SPynCas9
|
| pAC1445-pmax-dCas9 | dCas9 (Synthetic) | CAGGS + chim intron | |
Cheng
|
pAC1445
(unpublished)
|
dCas9 expressed in pmax vector
pAC90-pmax-DEST
|
| pAAVS1-NDi-CRISPRi (Gen1) | dCas9-KRAB-P2A-mCherry (Synthetic), rtTA (Synthetic) | TRE3G, CAG | Neomycin (select with G418) |
Conklin
| CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs.
Cell Stem Cell. 2016 Apr 7;18(4):541-53. doi: 10.1016/j.stem.2016.01.022. Epub 2016 Mar 10.
|
Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker
pAAVS1
|
| pAAVS1-NDi-CRISPRi (Gen2) | dCas9-KRAB (Synthetic), rtTA (Synthetic) | TRE3G, CAG | Neomycin (select with G418) |
Conklin
| CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs.
Cell Stem Cell. 2016 Apr 7;18(4):541-53. doi: 10.1016/j.stem.2016.01.022. Epub 2016 Mar 10.
|
Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus
pAAVS1
|
| pAAVS1-NC-CRISPRi (Gen3) | dCas9-KRAB (Synthetic) | CAG | Neomycin (select with G418) |
Conklin
| CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs.
Cell Stem Cell. 2016 Apr 7;18(4):541-53. doi: 10.1016/j.stem.2016.01.022. Epub 2016 Mar 10.
|
Constitutive CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus
pAAVS1
|
| pHAGE-TO-dCas9 | Sp dCas9 (Synthetic) | CMV-TO | |
Pederson
| Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow.
Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
|
dCas9
pHAGE-DEST
|
| WN10150 | dead (908A) AsCpf1 | | Neomycin (select with G418) |
Welker
| Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants.
Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815.
|
Expresses dead (908A) AsCpf1
pcDNA3.1
|
| CSII-U6-gRNA-CBh-3xFLAG-PA-dCas9-P2A-Puro | sgRNA and dCas9 from pX330 (Other) | U6 for sgRNA and CBh for dCas9 | Puromycin |
Kimura
|
Dr. Sekita CRISPR/Cas9
(unpublished)
|
Lentivirus vector to express guideRNA and dCas9 with puro resistant gene
CSII
|
| pLV-dCas9-KRAB-PGK-HygR | humanized dead Cas9 KRAB (Other), aminoglycoside phosphotransferase from E. coli (Other) | Human Ubiquitin C Promoter, mouse phosphoglycerate kinase 1 promoter | Hygromycin |
Gersbach
| CRISPR-Cas9 epigenome editing enables high-throughput screening for functional regulatory elements in the human genome.
Nat Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853.
|
Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.
FUGW
|
| pSLQ2818 pPB: CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9 | ABI-tagBFP-Sp dCas9 (Arabidopsis thaliana), PYL1-KRAB (Arabidopsis thaliana) | PGK, CAG | Puromycin |
Qi
| Complex transcriptional modulation with orthogonal and inducible dCas9 regulators.
Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
|
Expresses ABA-inducible KRAB-Sp dCas9
PB
|
| pSLQ2813 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-ABI-tagBFP-SpdCas9 | GAI-tagBFP-Sp dCas9 (Arabidopsis thaliana), GID1-KRAB (Arabidopsis thaliana) | PGK, CAG | Puromycin |
Qi
| Complex transcriptional modulation with orthogonal and inducible dCas9 regulators.
Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
|
Expresses GA-inducible KRAB-Sp dCas9
PB
|
| pSLQ2815 pPB: CAG-Puro-WPRE PGK-KRAB-tagBFP-SpdCas9 | KRAB-tagBFP-Sp dCas9 (Synthetic), Puro | PGK, CAG | Puromycin |
Qi
| Complex transcriptional modulation with orthogonal and inducible dCas9 regulators.
Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
|
Expresses direct fusion KRAB-Sp dCas9
PB
|
| pGH125_dCas9-Blast | dCas9 and Blasticidin resistance | | Blasticidin |
Bassik
| Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells.
Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038.
|
lentiviral expression vector for dCas9 with Blasticidin selectable marker
Addgene #61425
|
| TRE-KRAB-dCas9-IRES-BFP | KRAB-dCas9-IRES-BFP (Synthetic) | TRE3G | BFP |
Lander
| Systematic mapping of functional enhancer-promoter connections with CRISPR interference.
Science. 2016 Sep 29. pii: aag2445.
|
Lentiviral vector expressing a KRAB-dCas9 fusion protein and BFP
pHR
|
| TRE-KRAB-dCas9-IRES-GFP | KRAB-dCas9-IRES-GFP (Synthetic) | TRE3G | GFP |
Lander
| Systematic mapping of functional enhancer-promoter connections with CRISPR interference.
Science. 2016 Sep 29. pii: aag2445.
|
2nd generation lentiviral vector expressing a KRAB-dCas9 fusion protein and GFP
pHR
|
| pMH0001 | dCas9 (Other) | SFFV | BFP fluorescence |
Weissman
| A Multiplexed Single-Cell CRISPR Screening Platform Enables Systematic Dissection of the Unfolded Protein Response.
Cell. 2016 Dec 15;167(7):1867-1882.e21. doi: 10.1016/j.cell.2016.11.048.
|
UCOE-SFFV-dCas9-BFP-KRAB
pHR-SFFV-dCas9-BFP-KRAB
|
| pTE4565 | hMbCpf1(D986A) | CMV | Neomycin (select with G418) |
Welker
| Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants.
Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815.
|
Expresses inactive/dead, humanized MbCpf1 nuclease
pcDNA3.1
|
| pTE4889 | Fn crRNA, hFnCpf1(D917A) | human U6, CMV | Neomycin (select with G418) |
Welker
| Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants.
Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815.
|
Expresses FnCpf1 crRNA and inactive/dead, humanized FnCpf1 nuclease
pcDNA3.1
|
| Lenti-dCas9-KRAB-blast | dCas9 (Synthetic) | | Blasticidin |
Hon
| Multiplexed Engineering and Analysis of Combinatorial Enhancer Activity in Single Cells.
Mol Cell. 2017 Apr 20;66(2):285-299.e5. doi: 10.1016/j.molcel.2017.03.007. Epub 2017 Apr 13.
|
Plasmid expression dCas9 protein in fusion with KRAB domain
plenti
|
| pX330-HA-dSpCas9 | dead/inactive SpCas9 (Other) | Cbh | |
Welker
| Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage.
Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8.
|
Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)
pX330-like (without U6-sgRNA coding sequence)
|
| pX330-Flag-dSpCas9 | dead/inactive SpCas9 with FLAG tag (Other) | Cbh | |
Welker
| Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage.
Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8.
|
Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)
pX330-like (without U6-sgRNA coding sequence)
|
| pX330-Flag-deSpCas9 | dead/inactive eSpCas9 with FLAG tag (Other) | Cbh | |
Welker
| Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage.
Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8.
|
Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)
pX330-like (without U6-sgRNA coding sequence)
|
| pX330-Flag-dSpCas9-HF1 | dead/inactive SpCas9-HF1 with FLAG tag (Other) | Cbh | |
Welker
| Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage.
Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8.
|
Expression plasmid for human codon-optimized dead/inactive high-fidelity SpCas9-HF1 (without U6-sgRNA coding sequence)
pX330-like (without U6-sgRNA coding sequence)
|
| pX330-Flag-dHeFSpCas9 | dead/inactive HeFSpCas9 with FLAG tag (Other) | Cbh | |
Welker
| Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage.
Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8.
|
Expression plasmid for human codon-optimized dead/inactive increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)
pX330-like (without U6-sgRNA coding sequence)
|
| pLX_311-KRAB-dCas9 | dCas9 (Other) | EF1a | Blasticidin |
Hahn
| Complementary information derived from CRISPR Cas9 mediated gene deletion and suppression.
Nat Commun. 2017 May 23;8:15403. doi: 10.1038/ncomms15403.
|
for CRISPRi, lentiviral expression of KRAB-dCas9 and BlastR. Also called pXPR_121
pXPR
Tag
/ Fusion Protein- KRAB (N terminal on insert)
|
| pXPR_123 | dCas9 (Other) | EF1a | Blasticidin |
Root
| Orthologous CRISPR-Cas9 enzymes for combinatorial genetic screens.
Nat Biotechnol. 2018 Feb;36(2):179-189. doi: 10.1038/nbt.4048. Epub 2017 Dec 18.
|
for CRISPRi, lentiviral expression of dCas9-p300 2A BlastR.
pXPR
Tag
/ Fusion Protein- p300 (C terminal on insert)
|
| lenti-EF1a-dCas9-KRAB-Puro | dCas9-KRAB-T2A-Puro (Other) | EF-1a | Puromycin, Zeocin |
Brennand
| Evaluating Synthetic Activation and Repression of Neuropsychiatric-Related Genes in hiPSC-Derived NPCs, Neurons, and Astrocytes.
Stem Cell Reports. 2017 Aug 8;9(2):615-628. doi: 10.1016/j.stemcr.2017.06.012. Epub 2017 Jul 27.
|
3rd generation lenti vector encoding dCas9-KRAB with 2A puromycin resistance marker (EF1a-dCas9-KRAB-T2A-Puro-WPRE)
pLenti
|
| pHR-EF1a-dCas9-HA-BFP-KRAB-NLS | dCas9-HA-BFP-KRAB-NLS (Homo sapiens) | EF1a | TagBFP |
Corn
|
Corn Lab Cas9 plasmids
(unpublished)
|
Lentiviral expression of dCas9-HA-BFP-KRAB-NLS for CRISPRi driven by a hEF1alpha promoter
pHR
Tag
/ Fusion Protein- HA-TagBFP-KRAB-NLS (C terminal on insert)
|
| pCXLE-dCas9GFP-shP53 | dCas9GFP-shP53 (Other) | CAG | |
Otonkoski
| Human pluripotent reprogramming with CRISPR activators.
Nat Commun. 2018 Jul 6;9(1):2643. doi: 10.1038/s41467-018-05067-x.
|
EBNA episome plasmid for CAG promoter constitutive expression of dCas9-GFP. Includes p53 shRNA expression cassette.
pCXLE
|
| PB-tight-DDdCas9GFP-IRES-Neo | DDdCas9GFP-IRES-Neo (Other) | TRE-tight | Neomycin (select with G418) |
Otonkoski
| Human pluripotent reprogramming with CRISPR activators.
Nat Commun. 2018 Jul 6;9(1):2643. doi: 10.1038/s41467-018-05067-x.
|
PiggyBac construct with doxycyline inducible TRE-tight promoter expression of dCas9-GFP followed by IRES-Neomycin selection cassette.
PB-tight
|
| pAW91.dCas9 | dCas9 | | Blasticidin |
Young
| YY1 Is a Structural Regulator of Enhancer-Promoter Loops.
Cell. 2017 Dec 14;171(7):1573-1588.e28. doi: 10.1016/j.cell.2017.11.008. Epub 2017 Dec 7.
|
Expresses dCas9
dcas9-vp64
|
| MMW1578: CAG-human dLbCpf1(D832A)-NLS-3xHA | dLbCpf1 (D832A) (Other) | CAG | |
Joung
| Inducible and multiplex gene regulation using CRISPR-Cpf1-based transcription factors.
Nat Methods. 2017 Oct 30. doi: 10.1038/nmeth.4483.
|
Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1)
pCAG-GFP
|
| AAV CMV-dSaCas9-KRAB-bGHpA | dead S. aureus Cas9 KRAB (Other) | CMV | |
Gersbach
| RNA-guided transcriptional silencing in vivo with S. aureus CRISPR-Cas9 repressors.
Nat Commun. 2018 Apr 26;9(1):1674. doi: 10.1038/s41467-018-04048-4.
|
AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor
pAAV
|
| pLV hUbC-dSaCas9-KRAB-T2A-PuroR | dead S. aureus Cas9 KRAB T2A PuroR (Other) | hUbC | Puromycin |
Gersbach
| RNA-guided transcriptional silencing in vivo with S. aureus CRISPR-Cas9 repressors.
Nat Commun. 2018 Apr 26;9(1):1674. doi: 10.1038/s41467-018-04048-4.
|
Lentiviral vector with puro selection for expression of S. aureus dCas9-KRAB
FUGW
|
| SID4x-dCas9-KRAB | SID4x (Homo sapiens), KRAB (Homo sapiens) | | Neomycin (select with G418) |
Gertz
| Multiplex Enhancer Interference Reveals Collaborative Control of Gene Regulation by Estrogen Receptor alpha-Bound Enhancers.
Cell Syst. 2017 Oct 25;5(4):333-344.e5. doi: 10.1016/j.cels.2017.08.011. Epub 2017 Sep 27.
|
Expresses catalytically inactive dCas9 with N-terminal fusion of Sin3a Interacting Domain (SID) and C-terminal fusion of repressive KRAB domain
pAC95-pmax (Addgene: 48227)
|
| AAV-nEF-dCas9 | nEF-dCas9 | nEF | |
Belmonte
| In Vivo Target Gene Activation via CRISPR/Cas9-Mediated Trans-epigenetic Modulation.
Cell. 2017 Dec 14;171(7):1495-1507.e15. doi: 10.1016/j.cell.2017.10.025. Epub 2017 Dec 7.
|
Expresses SpdCas9 from the nEF promoter in AAV backbone
AAV
|
| pdCas9-NED | | CMV | |
Kiss
| Establishment of Cell Lines Stably Expressing dCas9-Fusions to Address Kinetics of Epigenetic Editing.
Methods Mol Biol. 2018;1767:395-415. doi: 10.1007/978-1-4939-7774-1_22.
|
Mammalian vector for expressing effector domains fused to dCas9.
pMLM3705 (Addgene plasmid #47754).
Tag
/ Fusion Protein- dCas9, SV40 NLS, 3xFLAG (N terminal on backbone)
|
| pHAGE EF1α dCas9-NED | | EF-1α | Puromycin |
Kiss
| Establishment of Cell Lines Stably Expressing dCas9-Fusions to Address Kinetics of Epigenetic Editing.
Methods Mol Biol. 2018;1767:395-415. doi: 10.1007/978-1-4939-7774-1_22.
|
Lentiviral vector for expressing effector domains fused to dCas9.
pHAGE EF1α dCas9-VP64 (Addgene plasmid #50918)
Tag
/ Fusion Protein- 3xHA (N terminal on backbone)
|
| dCas9-KRAB | dCas9-KRAB (Synthetic) | CMV | |
Church
| An enhanced CRISPR repressor for targeted mammalian gene regulation.
Nat Methods. 2018 Jul 16. pii: 10.1038/s41592-018-0048-5. doi: 10.1038/s41592-018-0048-5.
|
Conventional dCas9 repressor-dCas9-KRAB
pcDNA3.3_TOPO
|
| dCas9-KRAB-MeCP2 | dCas9-KRAB-MeCP2 (Synthetic) | CMV | |
Church
| An enhanced CRISPR repressor for targeted mammalian gene regulation.
Nat Methods. 2018 Jul 16. pii: 10.1038/s41592-018-0048-5. doi: 10.1038/s41592-018-0048-5.
|
Improved dCas9 repressor-dCas9-KRAB-MeCP2
pcDNA3.3_TOPO
|
| pB-CAGGS-dCas9-KRAB | dCas9-KRAB (Synthetic) | CAGGS | Blasticidin |
Church
| An enhanced CRISPR repressor for targeted mammalian gene regulation.
Nat Methods. 2018 Jul 16. pii: 10.1038/s41592-018-0048-5. doi: 10.1038/s41592-018-0048-5.
|
PiggyBac compatible plasmid expressing dCas9-KRAB
pB-CAGGS
|
| pB-CAGGS-dCas9 | dCas9 (Synthetic) | CAGGS | Blasticidin |
Church
| An enhanced CRISPR repressor for targeted mammalian gene regulation.
Nat Methods. 2018 Jul 16. pii: 10.1038/s41592-018-0048-5. doi: 10.1038/s41592-018-0048-5.
|
PiggyBac compatible plasmid expressing dCas9
pB-CAGGS
|
| pB-CAGGS-dCas9-KRAB-MeCP2 | dCas9-KRAB-MeCP2 (Synthetic) | CAGGS | Blasticidin |
Church
| An enhanced CRISPR repressor for targeted mammalian gene regulation.
Nat Methods. 2018 Jul 16. pii: 10.1038/s41592-018-0048-5. doi: 10.1038/s41592-018-0048-5.
|
PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2
pB-CAGGS
|
| pCR1063 | dCas9-KRAB (Homo sapiens) | | |
Corn
| CRISPR-Cas9 genome editing in human cells occurs via the Fanconi anemia pathway.
Nat Genet. 2018 Aug;50(8):1132-1139. doi: 10.1038/s41588-018-0174-0. Epub 2018 Jul 27.
|
Expresses dCas9-KRAB tagged with mCherry: pHR-hEF1a-dCas9-mcherry-KRAB-mPGK-Hyg
pLentiXI
Tag
/ Fusion Protein- mCherry (C terminal on insert)
|
| pLL7 lenti-dcas9-3xflag-blast | dCas9(D10A, N863A)-T2A-Blast (Synthetic) | EF1a | Blasticidin |
Meng
| Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation.
Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090.
|
lenti vector encoding dcas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-dCas9-3Xflag-T2A-Blast-WPRE)
lenti dCAS-VP64_Blast
|
| KRAB-dCas9 | KRAB (Homo sapiens) | CMV | Neomycin (select with G418) |
Segal
| dCas9-based epigenome editing suggests acquisition of histone methylation is not sufficient for target gene repression.
Nucleic Acids Res. 2017 Sep 29;45(17):9901-9916. doi: 10.1093/nar/gkx578.
|
KRAB fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
pcDNA3.3-TOPO
|
| lentiCRISPR v2-dCas9 | dCas9 (Synthetic), Porumycin resistance (Other) | EFS-NS, EFS-NS | Puromycin |
Gilmore
|
lentiviral expression plasmid for gRNA, puromycin, and dCas9
(unpublished)
|
Lentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2
Custom
|
| pXPR_118 | dCas9 (Other) | | Hygromycin |
Doench
|
Doench CRISPR plasmids
(unpublished)
|
Lentiviral vector expressing dCas9
pXPR_118
|
CRISPR Plasmids: Interfere
